Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVB87_RS00615 Genome accession   NZ_CP089925
Coordinates   126207..127337 (-) Length   376 a.a.
NCBI ID   WP_232898993.1    Uniprot ID   -
Organism   Lysobacter sp. KIS68-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 121207..132337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVB87_RS00595 (LVB87_00595) mgtE 122706..124067 (+) 1362 WP_232898989.1 magnesium transporter -
  LVB87_RS00600 (LVB87_00600) - 124078..125025 (-) 948 WP_232898990.1 aspartate carbamoyltransferase catalytic subunit -
  LVB87_RS00605 (LVB87_00605) ruvX 125022..125534 (-) 513 WP_232898991.1 Holliday junction resolvase RuvX -
  LVB87_RS00610 (LVB87_00610) - 125549..126115 (-) 567 WP_232898992.1 YqgE/AlgH family protein -
  LVB87_RS00615 (LVB87_00615) pilU 126207..127337 (-) 1131 WP_232898993.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVB87_RS00620 (LVB87_00620) pilT 127356..128393 (-) 1038 WP_232898994.1 type IV pilus twitching motility protein PilT Machinery gene
  LVB87_RS00625 (LVB87_00625) - 128551..129216 (+) 666 WP_232900581.1 YggS family pyridoxal phosphate-dependent enzyme -
  LVB87_RS00630 (LVB87_00630) proC 129227..130054 (+) 828 WP_232898995.1 pyrroline-5-carboxylate reductase -
  LVB87_RS00635 (LVB87_00635) - 130041..130505 (+) 465 WP_232898996.1 DUF4426 domain-containing protein -
  LVB87_RS00640 (LVB87_00640) - 130510..131301 (-) 792 WP_232898997.1 ABC transporter substrate-binding protein -
  LVB87_RS00645 (LVB87_00645) pdxH 131305..131904 (-) 600 WP_232898998.1 pyridoxamine 5'-phosphate oxidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41801.09 Da        Isoelectric Point: 6.8286

>NTDB_id=639122 LVB87_RS00615 WP_232898993.1 126207..127337(-) (pilU) [Lysobacter sp. KIS68-7]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMNPQQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPSVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKARRVSMEILLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLAQGLDGVEVAEAR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=639122 LVB87_RS00615 WP_232898993.1 126207..127337(-) (pilU) [Lysobacter sp. KIS68-7]
ATGAGCAGCATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACGGCCGGCAT
GCCGCCCTCGATGAAGGTCCACGGCAAGATTTCGCCGATCACGCAGAACCCGCTCACGCCGCAGCAGAGCCGCGACCTGG
TTCTCAACGTGATGAACCCGCAGCAGCGCGAGGAGTTCGAAAAGACGCACGAGTGCAACTTCGCGATCGGCGTGGCCGGC
GTGGGTCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTCCTGCGCCGCATCGAGACCAAGATCCC
GAGCGTGGAAGAGCTGAACCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTGGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTCGCGGCGATGATCGGCTACCGCAACCAGAACTCGACGGGCCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGACGCGGCCCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCGGCCAGTTGCTGATGGACCTCTCGCTCAACCTCAAGGGCGTGGT
GGCGCAGCAGCTGATCCCGACGCCCGACGGCAAGGCGCGCCGCGTGTCGATGGAAATCCTGCTGGGCACGCCGCTCGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAATCCACCAACCTCGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCTCCTACGAGGACGCGCTGCGCTACGCCGACTCGGCGAACGA
AGTGCGCCTGCGCATCAAGCTGGCGCAGGGCGGCGACGCACGGACGCTGGCGCAGGGCCTCGACGGCGTGGAAGTCGCCG
AAGCCCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

97.606

0.662
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.815

90.691

0.388
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362