Detailed information NTG0636412

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LUX28_RS13475
• Genome accession: NZ_CP089310
• Coordinates: 2715718..2716332 (-)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain A4P130
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2710718..2721332

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LUX28_RS13445 (LUX28_13445)	rpsT	2711018..2711284 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LUX28_RS13450 (LUX28_13450)	holA	2711301..2712342 (-)	1042	Protein_2607	DNA polymerase III subunit delta	-
LUX28_RS13455 (LUX28_13455)	-	2712382..2712536 (-)	155	Protein_2608	hypothetical protein	-
LUX28_RS13460 (LUX28_13460)	-	2712577..2712711 (+)	135	WP_003152870.1	YqzM family protein	-
LUX28_RS13465 (LUX28_13465)	comEC	2712730..2715081 (-)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LUX28_RS13470 (LUX28_13470)	-	2715082..2715651 (-)	570	WP_003152868.1	ComE operon protein 2	-
LUX28_RS13475 (LUX28_13475)	comEA	2715718..2716332 (-)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
LUX28_RS13480 (LUX28_13480)	comER	2716391..2717212 (+)	822	WP_015417855.1	late competence protein ComER	-
LUX28_RS13485 (LUX28_13485)	-	2717281..2718018 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
LUX28_RS13490 (LUX28_13490)	rsfS	2718015..2718371 (-)	357	WP_007408260.1	ribosome silencing factor	-
LUX28_RS13495 (LUX28_13495)	yqeK	2718389..2718949 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LUX28_RS13500 (LUX28_13500)	-	2718939..2719508 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
LUX28_RS13505 (LUX28_13505)	yhbY	2719519..2719809 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LUX28_RS13510 (LUX28_13510)	aroE	2719803..2720645 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=636412 LUX28_RS13475 WP_053573180.1 2715718..2716332(-) (comEA) [Bacillus velezensis strain A4P130]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=636412 LUX28_RS13475 WP_053573180.1 2715718..2716332(-) (comEA) [Bacillus velezensis strain A4P130]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363