Detailed information NTG0636125

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LT232_RS00450
• Genome accession: NZ_CP089287
• Coordinates: 69530..70144 (+)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GSBZ09
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 64530..75144

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LT232_RS00415 (LT232_00415)	aroE	65217..66059 (+)	843	WP_094031849.1	shikimate dehydrogenase	-
LT232_RS00420 (LT232_00420)	yhbY	66053..66343 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
LT232_RS00425 (LT232_00425)	-	66354..66923 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
LT232_RS00430 (LT232_00430)	yqeK	66913..67473 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LT232_RS00435 (LT232_00435)	rsfS	67491..67847 (+)	357	WP_007408260.1	ribosome silencing factor	-
LT232_RS00440 (LT232_00440)	-	67844..68581 (+)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
LT232_RS00445 (LT232_00445)	comER	68650..69471 (-)	822	WP_012118027.1	late competence protein ComER	-
LT232_RS00450 (LT232_00450)	comEA	69530..70144 (+)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
LT232_RS00455 (LT232_00455)	-	70211..70780 (+)	570	WP_094031848.1	ComE operon protein 2	-
LT232_RS00460 (LT232_00460)	comEC	70781..73132 (+)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LT232_RS00465 (LT232_00465)	-	73151..73285 (-)	135	WP_003152870.1	YqzM family protein	-
LT232_RS00470 (LT232_00470)	-	73326..73477 (+)	152	Protein_95	hypothetical protein	-
LT232_RS00475 (LT232_00475)	holA	73517..74558 (+)	1042	Protein_96	DNA polymerase III subunit delta	-
LT232_RS00480 (LT232_00480)	rpsT	74575..74841 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=636125 LT232_RS00450 WP_043021637.1 69530..70144(+) (comEA) [Bacillus velezensis strain GSBZ09]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=636125 LT232_RS00450 WP_043021637.1 69530..70144(+) (comEA) [Bacillus velezensis strain GSBZ09]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363