Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LSP22_RS07495 Genome accession   NZ_CP089064
Coordinates   1594726..1595262 (-) Length   178 a.a.
NCBI ID   WP_277989274.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain UNC_PaerCF35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1589726..1600262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSP22_RS07475 (LSP22_07475) - 1590302..1592557 (-) 2256 WP_023100227.1 DUF1631 domain-containing protein -
  LSP22_RS07480 (LSP22_07480) nadC 1592843..1593691 (+) 849 WP_021264134.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LSP22_RS07490 (LSP22_07490) tfpZ 1593924..1594661 (-) 738 WP_023100229.1 TfpX/TfpZ family type IV pilin accessory protein -
  LSP22_RS07495 (LSP22_07495) pilE 1594726..1595262 (-) 537 WP_277989274.1 pilin Machinery gene
  LSP22_RS07505 (LSP22_07505) pilB 1595489..1597192 (+) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LSP22_RS07510 (LSP22_07510) pilC 1597196..1598413 (+) 1218 WP_023103506.1 type II secretion system F family protein Machinery gene
  LSP22_RS07515 (LSP22_07515) pilD 1598416..1599288 (+) 873 WP_231822116.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  LSP22_RS07520 (LSP22_07520) coaE 1599285..1599896 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  LSP22_RS07525 (LSP22_07525) yacG 1599893..1600093 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18308.81 Da        Isoelectric Point: 4.9984

>NTDB_id=635009 LSP22_RS07495 WP_277989274.1 1594726..1595262(-) (pilE) [Pseudomonas aeruginosa strain UNC_PaerCF35]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLVLAEPAKLMISTDGSASTADLTRATTTWNQQSNNLGA
SSKYVTSVLMDAGNTGVITITYVADQVGLPTAGNTLILSPYINDGNSRTALATAVAAGTRGTIDWACTSASNATATAQGF
TGMAAGSVPQEFAPAQCR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=635009 LSP22_RS07495 WP_277989274.1 1594726..1595262(-) (pilE) [Pseudomonas aeruginosa strain UNC_PaerCF35]
ATGAAAGCTCAAAAAGGCTTCACTTTGATCGAGCTGATGATCGTTGTAGCGATCATCGGTATTCTGGCTGCGATTGCTCT
GCCGGCCTATCAGGACTACACCGTTCGTACCCGTGTCAGTGAAGGTCTGGTTCTGGCTGAGCCCGCCAAACTGATGATCT
CCACGGATGGAAGTGCCTCTACTGCTGATCTGACTCGCGCAACCACTACTTGGAACCAGCAATCCAATAATCTGGGTGCA
TCCAGTAAGTACGTAACATCTGTTTTGATGGATGCTGGTAACACCGGTGTTATCACTATTACGTATGTCGCTGACCAAGT
GGGCTTGCCGACTGCCGGTAACACTCTGATTCTGTCTCCCTATATCAATGATGGTAACAGTCGCACTGCTCTGGCGACTG
CAGTGGCTGCTGGCACTCGTGGCACCATTGATTGGGCTTGCACTTCGGCATCGAACGCGACTGCCACTGCTCAGGGCTTC
ACCGGCATGGCAGCTGGTAGTGTGCCGCAAGAGTTTGCTCCTGCTCAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.719

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

41.209

100

0.421
  pilA2 Legionella pneumophila strain ERS1305867

40.341

98.876

0.399
  pilA2 Legionella pneumophila str. Paris

39.773

98.876

0.393