Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   CSB63_RS08965 Genome accession   NZ_CP089060
Coordinates   1692319..1692756 (-) Length   145 a.a.
NCBI ID   WP_011681686.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain CH8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1687319..1697756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB63_RS08930 (CSB63_1879) - 1687384..1688046 (-) 663 WP_014727719.1 CPBP family intramembrane glutamic endopeptidase -
  CSB63_RS08935 (CSB63_1880) - 1688143..1688586 (-) 444 WP_041829335.1 hypothetical protein -
  CSB63_RS08940 (CSB63_1881) - 1688660..1689079 (-) 420 WP_014727721.1 CPBP family intramembrane glutamic endopeptidase -
  CSB63_RS08945 (CSB63_1883) - 1689328..1689525 (-) 198 WP_014727723.1 helix-turn-helix transcriptional regulator -
  CSB63_RS08950 (CSB63_1884) - 1689774..1690967 (-) 1194 WP_014608740.1 acetate kinase -
  CSB63_RS08955 (CSB63_1885) comYH 1691023..1691979 (-) 957 WP_011681684.1 class I SAM-dependent methyltransferase Machinery gene
  CSB63_RS08960 (CSB63_1886) comGG 1692024..1692341 (-) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  CSB63_RS08965 (CSB63_1887) comYF 1692319..1692756 (-) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  CSB63_RS08970 comGE 1692740..1693033 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  CSB63_RS08975 (CSB63_1889) comYD 1693005..1693433 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  CSB63_RS08980 (CSB63_1890) comYC 1693393..1693719 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  CSB63_RS08985 (CSB63_1891) comYB 1693716..1694816 (-) 1101 WP_134973504.1 competence type IV pilus assembly protein ComGB Machinery gene
  CSB63_RS08990 (CSB63_1892) - 1694698..1695390 (-) 693 Protein_1709 ATPase, T2SS/T4P/T4SS family -
  CSB63_RS08995 (CSB63_1893) - 1695448..1696800 (-) 1353 WP_134973005.1 IS66-like element short variant transposase -
  CSB63_RS09000 (CSB63_1894) - 1696754..1696960 (-) 207 WP_134973004.1 transposase -
  CSB63_RS09005 (CSB63_1895) tnpB 1697011..1697361 (-) 351 WP_014295152.1 IS66 family insertion sequence element accessory protein TnpB -
  CSB63_RS09010 (CSB63_1896) - 1697342..1697527 (-) 186 WP_134973003.1 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16375.85 Da        Isoelectric Point: 8.4500

>NTDB_id=634893 CSB63_RS08965 WP_011681686.1 1692319..1692756(-) (comYF) [Streptococcus thermophilus strain CH8]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVRQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNELQRSFVYAFEKTG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=634893 CSB63_RS08965 WP_011681686.1 1692319..1692756(-) (comYF) [Streptococcus thermophilus strain CH8]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCAGGTAAGACAACAAGAAAACCAAGATTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAAGTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGAACTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(39-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

58.156

97.241

0.566
  comYF Streptococcus mutans UA159

57.447

97.241

0.559
  comGF/cglF Streptococcus pneumoniae D39

49.286

96.552

0.476
  comGF/cglF Streptococcus pneumoniae Rx1

49.286

96.552

0.476
  comGF/cglF Streptococcus pneumoniae R6

49.286

96.552

0.476
  comGF/cglF Streptococcus pneumoniae TIGR4

49.286

96.552

0.476
  comGF/cglF Streptococcus mitis NCTC 12261

47.518

97.241

0.462
  comGF/cglF Streptococcus mitis SK321

46.809

97.241

0.455
  comGF Lactococcus lactis subsp. cremoris KW2

45.324

95.862

0.434