Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LSO59_RS15385 Genome accession   NZ_CP089038
Coordinates   3288623..3290335 (+) Length   570 a.a.
NCBI ID   WP_262546817.1    Uniprot ID   -
Organism   Acinetobacter ursingii strain RIVM_C011601     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3283623..3295335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO59_RS15350 (LSO59_15325) nusA 3284476..3285960 (-) 1485 WP_004994250.1 transcription termination factor NusA -
  LSO59_RS15355 (LSO59_15330) rimP 3285996..3286520 (-) 525 WP_004991666.1 ribosome maturation factor RimP -
  LSO59_RS15375 (LSO59_15350) secG 3287206..3287535 (-) 330 WP_004991669.1 preprotein translocase subunit SecG -
  LSO59_RS15380 (LSO59_15355) tpiA 3287545..3288342 (-) 798 WP_005001192.1 triose-phosphate isomerase -
  LSO59_RS15385 (LSO59_15360) pilB 3288623..3290335 (+) 1713 WP_262546817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LSO59_RS15390 (LSO59_15365) pilC 3290365..3291591 (+) 1227 WP_151832276.1 type II secretion system F family protein Machinery gene
  LSO59_RS15395 pilD 3291591..3292451 (+) 861 WP_005001186.1 prepilin peptidase Machinery gene
  LSO59_RS15400 (LSO59_15380) coaE 3292454..3293047 (+) 594 WP_005001184.1 dephospho-CoA kinase -
  LSO59_RS15405 (LSO59_15385) - 3293029..3293952 (-) 924 WP_005001182.1 DMT family transporter -
  LSO59_RS15410 (LSO59_15390) rlmB 3293993..3294742 (-) 750 WP_004994270.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LSO59_RS15415 (LSO59_15395) - 3294862..3295188 (-) 327 WP_004991681.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63272.72 Da        Isoelectric Point: 5.1511

>NTDB_id=634747 LSO59_RS15385 WP_262546817.1 3288623..3290335(+) (pilB) [Acinetobacter ursingii strain RIVM_C011601]
MSVLQASPKFSGFIRRIVEDGRVSAATMQTAIDTAKKSKQDIVAYMIEHLGISALTLAETISIEFGEPLFDLAVYDAALL
PRDLVDEKLIQKHHVVPLMHRGHVLYVATSNPTNIEALDAIRFNSKLNIEPIIVEHDKLTRLLEKEFAESDNFAFDGEDF
DIDVHVEEVNSDETDNEPPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLATRIAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMDALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTENSNISTAEDPVEINLEGVNQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCGQCKQAVDIPRNSLLEMGF
TEQDLVHPDFQIYQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=634747 LSO59_RS15385 WP_262546817.1 3288623..3290335(+) (pilB) [Acinetobacter ursingii strain RIVM_C011601]
ATGTCAGTATTACAAGCATCTCCAAAATTTTCGGGGTTCATTCGGCGAATAGTCGAAGACGGGCGTGTAAGCGCAGCAAC
CATGCAAACTGCGATCGATACGGCAAAAAAATCGAAGCAGGATATCGTTGCCTACATGATTGAACATTTGGGGATCTCAG
CCCTAACTCTTGCTGAAACCATTTCTATTGAATTTGGGGAACCCCTTTTTGATTTGGCTGTTTACGATGCTGCATTATTA
CCACGCGACCTAGTAGATGAAAAACTGATTCAAAAGCATCATGTTGTTCCTCTTATGCATCGAGGTCATGTACTTTACGT
CGCAACCAGCAATCCAACTAATATTGAAGCACTCGATGCGATTCGTTTTAACAGTAAGCTCAATATTGAGCCGATTATTG
TTGAACATGACAAATTAACACGCTTATTGGAAAAGGAATTTGCCGAAAGCGATAATTTTGCATTCGATGGTGAAGACTTC
GATATTGATGTCCATGTAGAAGAAGTCAATTCAGACGAAACAGATAATGAGCCCCCGCAGGGTGATGAAGCCCCAATTGT
AAAATATATTAATAAGTTGCTCATTGATGCGATTCGTATGGGTGCATCAGATTTACATTTTGAACCGTATGAAAAAATGT
ATCGCGTGCGTTACCGTGTAGATGGAGTATTACGTCAGATCGCCAATCCTCCCCTCCAGTTAGCCACCCGAATTGCATCG
CGCTTAAAAGTCATGTCACAAATGGATATTTCAGAAAAGCGTGTTCCACAAGATGGTCGTATCAAACTTAAACTTTCTAA
GACTAAAGCCATCGATTTCCGTGTGAACTCTTTACCTACCTTGTTTGGTGAAAAGCTAGTACTGCGTATTTTAGATCCGT
CTAGTGCTATGTTGGGTATCGATGCGCTGGGTTATGAAGAAGAACAAAAAGCGCTATTTATGGATGCGCTGGACAAACCA
CAGGGAATGCTGCTGATTACTGGACCAACAGGTTCTGGTAAAACTGTTTCACTTTATACAGGCCTCAATATTCTAAACAC
TGAAAATTCTAATATTTCAACTGCTGAAGACCCTGTGGAAATTAACCTTGAAGGTGTTAATCAGGTGAATGTTAACCCCA
AGGTGGGCTTGACCTTTGCCGCTGCGCTAAAGTCTTTTTTACGTCAGGATCCTGACATTATCATGGTGGGTGAGATTCGT
GATCTGGAAACAGCAGAAATCGCAATCAAGGCCGCACAAACGGGCCACATGGTCATGTCTACGCTGCATACTAACAGTGC
GCCAGAAACATTAACGCGTTTACGTAATATGGGTGTACCCTCATTTAATATTGCAACATCAGTTAATCTAGTGATTGCAC
AACGTCTGGCACGGCGCTTATGTGGTCAATGTAAACAAGCGGTGGACATTCCTAGAAATAGTTTGCTAGAAATGGGATTT
ACCGAACAAGATTTAGTACATCCTGACTTTCAGATTTATCAACCTGTCGGTTGCTCTGAATGTCGCGAAGGTTATAAAGG
TCGTGTCGGGATTTACGAAGTCATGAAAGTCACACCTGAAATTTCGAAAATTATTATGGAAGATGGCAATGCACTTGAAA
TTGCAGCGGCATCGGAAAAACTGGGGTTTAATAATCTTCGTCGTTCAGGTCTTAAAAAGGTGATGCAAGGGGTCACTTCT
TTACAGGAAGTCAACCGCGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

84.035

100

0.84
  pilB Acinetobacter baylyi ADP1

80.351

100

0.804
  pilB Legionella pneumophila strain ERS1305867

53.789

94.912

0.511
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio cholerae strain A1552

49.365

96.667

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

97.544

0.477
  pilB Vibrio campbellii strain DS40M4

51.064

90.702

0.463