Detailed information NTG0634134

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: LRR79_RS07955
• Genome accession: NZ_CP088953
• Coordinates: 1931478..1933190 (-)
• Length: 570 a.a.
• NCBI ID: WP_231759821.1
• Uniprot ID: -
• Organism: Microbulbifer elongatus strain DSM 6810
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1934081..1945925	1931478..1933190	flank	891

Gene organization within MGE regions

Location: 1931478..1945925

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRR79_RS07955	pilB	1931478..1933190 (-)	1713	WP_231759821.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LRR79_RS07960	pilE	1933607..1934020 (+)	414	WP_231759822.1	pilin	Machinery gene
LRR79_RS07965	-	1934081..1936057 (+)	1977	WP_231759823.1	sulfatase-like hydrolase/transferase	-
LRR79_RS07970	-	1936054..1937163 (+)	1110	WP_231759824.1	hypothetical protein	-
LRR79_RS07975	-	1937214..1937573 (+)	360	WP_231759825.1	hypothetical protein	-
LRR79_RS07980	-	1937628..1938836 (+)	1209	WP_231759826.1	glycosyltransferase family 2 protein	-
LRR79_RS07985	-	1938881..1939717 (+)	837	WP_231759827.1	glycosyltransferase family 2 protein	-
LRR79_RS07990	-	1939849..1941045 (+)	1197	WP_231759828.1	lipopolysaccharide biosynthesis protein	-
LRR79_RS07995	-	1941067..1942092 (+)	1026	WP_231759829.1	Stealth CR1 domain-containing protein	-
LRR79_RS17375	tnpA	1942625..1942849 (+)	225	WP_407665243.1	IS66 family insertion sequence element accessory protein TnpA	-
LRR79_RS08000	tnpB	1942882..1943061 (+)	180	WP_269455115.1	IS66 family insertion sequence element accessory protein TnpB	-
LRR79_RS08005	-	1943154..1944092 (+)	939	WP_231760000.1	helix-turn-helix domain-containing protein	-
LRR79_RS08010	-	1944208..1944639 (+)	432	WP_231759831.1	nucleotidyltransferase substrate binding protein	-
LRR79_RS08015	-	1944623..1944952 (+)	330	WP_231759832.1	nucleotidyltransferase domain-containing protein	-
LRR79_RS08020	hepT	1945099..1945518 (-)	420	WP_231759833.1	type VII toxin-antitoxin system HepT family RNase toxin	-
LRR79_RS08025	mntA	1945518..1945925 (-)	408	WP_231759834.1	type VII toxin-antitoxin system MntA family adenylyltransferase antitoxin	-

Sequence

Protein

• Length: 570 a.a.
• Molecular weight: 62324.02 Da
• Isoelectric Point: 5.0916

>NTDB_id=634134 LRR79_RS07955 WP_231759821.1 1931478..1933190(-) (pilB) [Microbulbifer elongatus strain DSM 6810]
MSSTSLSGLAKRLVIDKALDEPTAQSAVKAAKREGHSFAQHAVEAKLIKSRELANIASQAFGSPLFDLSCYNFDLLPKDV
VDEKLITKHFALPLYKRGSRLFIAVADPTNLAGLDEINFNTGLNTEAVLVEADKLSKAIESFLSNSGDISGGLEGLDDDD
LDSLDVDASDQTRDDEGDVGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKAYRVRLRTDGVLHEVARPPIQLATRISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEEEQKKIYLDALGQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSNKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCGECKKPTELPEEVLQEEGF
EAVTIPKTEWQIYQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQAQKEGFNNLRTSALRKVVMGVTS
LEEANRVTND

Nucleotide

• Length: 1713 bp

>NTDB_id=634134 LRR79_RS07955 WP_231759821.1 1931478..1933190(-) (pilB) [Microbulbifer elongatus strain DSM 6810]
ATGAGCAGTACCTCCCTCAGTGGACTTGCGAAGCGATTAGTTATCGACAAAGCCTTGGACGAACCTACAGCCCAGTCAGC
TGTAAAAGCAGCCAAGCGGGAAGGGCACAGCTTTGCTCAGCACGCTGTGGAGGCCAAGCTGATCAAGAGTCGAGAGCTTG
CTAATATTGCCAGCCAAGCGTTTGGCTCTCCGCTATTTGACCTCTCCTGCTACAATTTTGACCTTTTACCCAAAGATGTG
GTTGATGAAAAATTAATCACCAAGCACTTTGCACTGCCACTGTATAAGCGGGGAAGCAGGCTCTTTATCGCGGTGGCAGA
TCCTACTAATCTCGCGGGCCTTGATGAGATCAACTTTAATACCGGACTCAATACCGAGGCTGTCCTTGTAGAAGCAGATA
AGCTCTCCAAAGCTATCGAGAGTTTCCTCTCAAATAGCGGCGACATCAGTGGAGGACTGGAAGGGCTTGATGACGATGAC
TTGGACAGTCTAGATGTAGATGCGAGCGATCAGACACGTGACGACGAGGGAGATGTCGGGGGTGATGAAGCTCCGGTTGT
TCGATTCGTTAACAAGGTACTGCTGGATGCGATCCGTACGGGTGCATCAGACATTCATTTTGAGCCCTATGAAAAGGCTT
ACCGAGTACGACTGCGTACCGACGGTGTGCTGCACGAAGTGGCCCGTCCACCGATTCAACTCGCCACAAGAATTTCAGCG
CGCCTCAAAGTAATGTCCAAGATGGATATTTCTGAACGCCGTGTACCCCAGGATGGCCGTATCAAGATGAAGCTGTCCAA
AACCAAGGCCATCGACTTCCGGGTCAACAGCCTTCCTACCCTGTGGGGAGAAAAGATCGTACTGCGGATTCTCGACCCTT
CCTCGGCCAAGCTGGGTATTGACGCGCTGGGCTACGAGGAAGAGCAGAAGAAAATTTACTTAGACGCACTAGGACAACCC
CAAGGGATGATATTGGTTACCGGCCCAACCGGTTCGGGTAAAACCGTATCGCTGTATACCGGCCTAAACATCCTAAATAC
ACCAGAGCGCAACATCTCCACGGCAGAAGACCCTGTTGAGATCAACCTGGAGGGGATCAACCAGGTTAACGTCTCCAACA
AGGTAGGATTAAACTTTGCCGAGGCCTTGCGCTCTTTCCTTCGCCAAGACCCGGATATCGTAATGGTGGGCGAGATCCGA
GATCTTGAAACCGCAGAAATAGCGATCAAGGCTGCTCAGACCGGTCACTTGGTGCTTTCTACCTTACACACTAATTCCGC
CCCGGAAACGCTAACGCGATTAATGAATATGGGGGTCCCAACCTTCAATATTGCTACATCAGTGAGTGTCATTATCGCCC
AGCGTTTAGCACGGCGACTATGCGGCGAATGCAAAAAACCGACCGAGCTCCCGGAAGAAGTTTTACAAGAAGAAGGGTTC
GAAGCCGTTACCATCCCTAAAACCGAGTGGCAGATATACCAGCCCGTAGGCTGCGAACACTGCTCCAAAGGCTACAAAGG
GCGCGTAGGTGTGTATGAAGTAGTTCGCATCACCGACGGAATTTCGCGCATTATAATGGAAGGTGGCAATTCCATTCAGA
TTGCTGACCAAGCGCAAAAAGAAGGCTTCAACAACCTCAGAACCTCGGCTCTGCGCAAAGTGGTGATGGGAGTCACCAGC
CTGGAAGAAGCCAACCGGGTTACCAACGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II/IV secretion system protein (T2SSE) (192-459)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	60.457	99.825	0.604
pilB	Acinetobacter baylyi ADP1	60.354	99.123	0.598
pilB	Legionella pneumophila strain ERS1305867	56.063	99.825	0.56
pilB	Vibrio parahaemolyticus RIMD 2210633	54.579	95.789	0.523
pilB	Vibrio campbellii strain DS40M4	54.029	95.789	0.518
pilB	Vibrio cholerae strain A1552	53.48	95.789	0.512
pilF	Neisseria gonorrhoeae MS11	50	92.632	0.463
pilB/pilB1	Synechocystis sp. PCC 6803	38.25	100	0.414