Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LQF05_RS16050 Genome accession   NZ_CP088004
Coordinates   3451056..3452759 (-) Length   567 a.a.
NCBI ID   WP_270066808.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain SB-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3446056..3457759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQF05_RS16020 (LQF05_15995) - 3446239..3447279 (+) 1041 WP_270066805.1 NADP(H)-dependent aldo-keto reductase -
  LQF05_RS16025 (LQF05_16000) - 3447337..3448017 (+) 681 WP_270066806.1 energy-coupling factor ABC transporter permease -
  LQF05_RS16030 (LQF05_16005) yacG 3448030..3448227 (-) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  LQF05_RS16035 (LQF05_16010) coaE 3448224..3448832 (-) 609 WP_080940987.1 dephospho-CoA kinase -
  LQF05_RS16040 (LQF05_16015) pilD 3448964..3449833 (-) 870 WP_270066807.1 prepilin peptidase Machinery gene
  LQF05_RS16045 (LQF05_16020) pilC 3449836..3451053 (-) 1218 WP_265661083.1 type II secretion system F family protein Machinery gene
  LQF05_RS16050 (LQF05_16025) pilB 3451056..3452759 (-) 1704 WP_270066808.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LQF05_RS16055 (LQF05_16035) - 3453069..3453515 (+) 447 WP_270066809.1 pilin -
  LQF05_RS16060 (LQF05_16045) cysN 3454455..3456353 (-) 1899 WP_270066810.1 sulfate adenylyltransferase subunit CysN -
  LQF05_RS16065 (LQF05_16050) cysD 3456365..3457282 (-) 918 WP_011912238.1 sulfate adenylyltransferase subunit CysD -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62106.01 Da        Isoelectric Point: 5.4768

>NTDB_id=632304 LQF05_RS16050 WP_270066808.1 3451056..3452759(-) (pilB) [Stutzerimonas stutzeri strain SB-1]
MSENTSLSGLARQMVAAGVLDERTAQQAQAQAIRGKTPFVTYVVQNRLAKGRVIAELASEQFGVALLDLAALDKESLPRD
LVSEKLAKQHRVLPLSKRGTKLYIAVSDPANHQSVADIQFSTGLSIEPVLVEDDKLGEVIDKLYDSGATGLEDLSEVDLD
GVDVESVSDDGRTDDAGEVADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEVDVSRDVLLREGFP
EQKLGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPSLQRIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=632304 LQF05_RS16050 WP_270066808.1 3451056..3452759(-) (pilB) [Stutzerimonas stutzeri strain SB-1]
ATGAGCGAGAATACTTCCCTTTCCGGACTGGCGCGGCAGATGGTGGCAGCTGGTGTGCTGGACGAGAGGACGGCGCAGCA
GGCCCAGGCTCAGGCCATTCGCGGCAAGACACCCTTTGTCACCTACGTCGTCCAGAACCGGTTGGCCAAGGGCCGTGTCA
TTGCAGAACTCGCCTCCGAGCAGTTCGGTGTTGCGCTGCTGGATCTTGCTGCGCTGGATAAGGAGTCGCTGCCCAGGGAC
TTGGTGAGCGAGAAGCTGGCCAAGCAGCACCGGGTATTACCGCTCTCCAAGCGCGGCACCAAGCTGTATATCGCAGTTTC
CGACCCCGCCAACCATCAATCGGTTGCCGATATCCAGTTCAGCACCGGGCTTTCGATCGAGCCAGTACTGGTAGAAGACG
ATAAGCTCGGTGAGGTAATCGACAAGCTTTACGACAGTGGGGCTACTGGGCTGGAGGATCTGAGTGAGGTCGACTTAGAT
GGCGTAGATGTCGAGTCGGTTAGTGATGACGGTCGGACGGATGATGCAGGGGAGGTCGCGGACGATGCGCCCGTTGTGCG
CTTCGTCAACAAGATGTTGCTCGATGCCATTCGTGGAGGCTCATCTGATCTCCACTTTGAGCCTTATGAGAAAGCCTACC
GGGTACGGTTACGTACTGATGGCATTTTGCATGAGGTGGCGCGTCCTCCAATACAGTTGGCGTCCAGGATTTCTGCGCGC
CTCAAGGTGATGGCTGGGCTGGATATTTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAGCTCTCCAAGAG
CAAGGCCATCGATTTCCGGGTCAACACGCTACCGACCTTGTGGGGCGAAAAGATCGTGATGCGGATTCTTGATCCTTCGA
GCGCCCAAATGGGCATTGATGCGCTGGGGTATGAGGAGGATCAAAAAGAGCTGTATATGAATGCTCTGCGGCAACCGCAG
GGTATGATTCTCGTGACTGGTCCGACGGGCTCCGGTAAGACGGTATCCCTTTACACCGGGTTGAACATCCTCAACACCGT
CGATGTGAACATTTCGACGGCGGAGGATCCGGTAGAGATCAACCTCGAGGGTATCAACCAGGTCAACGTCAATCCTAAAC
AGGGCATGGACTTTTCTCAGGCCCTCCGTGCCTTTCTACGTCAGGACCCGGACATCATCATGGTAGGGGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCATATGGTGATGTCGACACTTCATACCAATAGCGCTGC
GGAAACCCTGACGCGTCTGCGCAACATGGGGGTGCCTGCGTTCAACATCGCGACCTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCACGAAAGCTCTGCAATAGCTGCAAGAAAGAGGTTGATGTCTCCCGTGATGTTTTGCTGCGCGAAGGGTTCCCT
GAGCAGAAGCTTGGAACCTTCAAGATTTATGGTCCGGTAGGTTGCGACAACTGCAAGGGCGGCTACAAGGGGCGCGTCGG
TATTTATGAAGTGGTTAAAAACACGCCATCGTTGCAGCGGATTATCATGGAAGACGGAAACTCCATCGATATTTCAACCC
AGATGCGCAAAGACGGCTTCAACGACCTGCGGACCTCGGCTTTGCTGAAAGCCATGCAGGGCGTGACCAGCCTCGAAGAA
GTCAATCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(14-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.284

99.471

0.6
  pilB Acinetobacter baylyi ADP1

58.97

99.295

0.586
  pilB Legionella pneumophila strain ERS1305867

55.965

100

0.563
  pilB Vibrio campbellii strain DS40M4

48.014

100

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

52.783

91.887

0.485
  pilF Neisseria gonorrhoeae MS11

48.845

99.295

0.485
  pilB Vibrio cholerae strain A1552

52.816

90.829

0.48