Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LP127_RS00975 Genome accession   NZ_CP087771
Coordinates   197478..199187 (+) Length   569 a.a.
NCBI ID   WP_078274627.1    Uniprot ID   A0AAQ2Q8U8
Organism   Moraxella bovis strain SAM109244     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 192478..204187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP127_RS00950 (LP127_00945) - 193227..193535 (+) 309 WP_078274632.1 DUF4212 domain-containing protein -
  LP127_RS00955 (LP127_00950) - 193538..195310 (+) 1773 WP_112741730.1 sodium:solute symporter family protein -
  LP127_RS00960 (LP127_00955) - 195472..195960 (+) 489 WP_078274630.1 hypothetical protein -
  LP127_RS00965 (LP127_00960) secG 196121..196417 (-) 297 WP_078274629.1 preprotein translocase subunit SecG -
  LP127_RS00970 (LP127_00965) tpiA 196428..197177 (-) 750 WP_078274628.1 triose-phosphate isomerase -
  LP127_RS00975 (LP127_00970) pilB 197478..199187 (+) 1710 WP_078274627.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LP127_RS00980 (LP127_00975) - 199285..200154 (-) 870 WP_078274626.1 MetQ/NlpA family lipoprotein -
  LP127_RS00985 (LP127_00980) - 200334..201023 (-) 690 WP_078274625.1 methionine ABC transporter permease -
  LP127_RS00990 (LP127_00985) metN 201013..202059 (-) 1047 WP_078274624.1 methionine ABC transporter ATP-binding protein MetN -
  LP127_RS00995 (LP127_00990) - 202643..203158 (+) 516 WP_078274623.1 hypothetical protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61936.17 Da        Isoelectric Point: 5.0575

>NTDB_id=631361 LP127_RS00975 WP_078274627.1 197478..199187(+) (pilB) [Moraxella bovis strain SAM109244]
MSAPNFGGLAHILVSNGLVAPDAMTKALTEAQQQSMAIVPYLVENKVAPANKIASLLAHEFGDPLFDLNALSPDAVNKEL
VDEKIINQYGALPLFKRGNKLFVGLSDPTRLDAIDAISFNAKLTVETIVVEEDKLKKMIENVYSGGGGLGNLGDVDLSGD
FDGGSGDEDDEGADVNSGGGEDAPVVKFVNKMLLDAIRMGASDLHFEPYEKSYRVRFRVDGVLQIINRPPVQLAGKIAAR
LKVMSQMDISERRAPQDGRIKLKISKTKAIDFRVNSCPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFLEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNTPETNISTAEDPVEINLEGINQVNVNPKVGLTFASALKAFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPTNVPKQSLLEMGFT
EEDLANPDNVIYEPVGCSECREGYKGRVGIYEVMKIYPSIARIIMEDGNAIQIKDEAMKHGFRDLRRSGIMKVLQGTTSI
QEMYRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=631361 LP127_RS00975 WP_078274627.1 197478..199187(+) (pilB) [Moraxella bovis strain SAM109244]
ATGTCCGCTCCGAATTTTGGTGGTTTGGCACACATTTTGGTGTCAAATGGCTTGGTTGCCCCTGATGCCATGACCAAGGC
TCTGACCGAAGCCCAGCAGCAAAGCATGGCGATTGTGCCATATTTGGTAGAAAACAAAGTTGCCCCTGCAAATAAGATTG
CCAGTCTGCTTGCTCATGAATTTGGCGACCCATTATTTGACCTAAATGCACTGTCTCCTGATGCGGTCAATAAAGAGCTG
GTTGATGAGAAAATCATTAATCAGTATGGGGCATTGCCACTCTTTAAACGGGGTAATAAACTTTTTGTGGGTTTGTCCGA
CCCCACCCGTCTAGATGCCATTGATGCCATCTCCTTTAACGCCAAGCTGACTGTTGAGACCATCGTTGTCGAAGAAGACA
AGCTCAAAAAGATGATTGAGAATGTCTACTCAGGCGGTGGCGGACTGGGCAACCTAGGCGATGTTGACCTGTCAGGGGAC
TTTGATGGTGGGTCAGGTGATGAAGATGATGAAGGTGCTGACGTCAATTCAGGCGGTGGTGAAGATGCCCCTGTGGTCAA
GTTCGTTAATAAAATGCTCCTTGATGCCATTCGTATGGGAGCATCGGATTTGCACTTTGAGCCGTATGAGAAGTCTTACC
GTGTGCGTTTTCGTGTGGATGGGGTTTTGCAAATCATTAATCGCCCACCAGTACAACTGGCAGGTAAAATCGCCGCTCGT
CTTAAAGTCATGTCGCAAATGGACATATCCGAACGCCGAGCTCCCCAGGATGGTCGTATTAAGCTAAAAATCTCCAAAAC
AAAAGCCATTGACTTTCGTGTGAACTCCTGCCCTACGCTCTTTGGCGAAAAGCTCGTACTGCGTATTCTTGACCCGTCAT
CAGCGATGCTTGGTATTGATGCACTCGGCTATGAGCCTGACCAAAAAGAGCTGTTCTTAGAAGCTCTTGATAAACCTCAA
GGCATGCTACTTATCACAGGTCCGACAGGTTCGGGTAAAACGGTCTCGCTCTATACTGGTCTTAACATCCTAAATACGCC
CGAGACCAACATCTCTACCGCCGAAGACCCTGTGGAAATTAACTTAGAAGGGATTAACCAAGTAAACGTCAACCCCAAAG
TGGGTCTCACGTTCGCCTCCGCCCTAAAAGCATTCCTACGTCAAGACCCTGACATCGTCATGGTCGGTGAGATTCGTGAT
TTGGAGACTGCCGAGATTGCCATTAAAGCCGCCCAAACGGGACACATGGTATTATCCACCCTGCACACCAACTCCGCTCC
TGAGACCCTAACTCGTCTGCGTAATATGGGCGTGGCATCGTTTAACATTGCCACATCGGTAAACCTAGTGATTGCCCAGC
GTCTGGCTCGCCGTTTGTGCAAAAACTGCAAAAAGCCGACCAACGTACCTAAGCAGAGCCTGCTAGAAATGGGCTTTACC
GAAGAAGATCTTGCCAATCCTGATAACGTCATCTATGAGCCTGTGGGCTGTTCTGAGTGTCGTGAAGGCTACAAGGGGCG
TGTGGGTATCTATGAAGTCATGAAAATCTACCCAAGTATCGCTCGGATTATCATGGAAGACGGTAATGCCATTCAAATCA
AAGACGAAGCGATGAAACACGGCTTTCGTGATTTGAGACGCTCGGGTATCATGAAAGTCTTGCAAGGCACGACCAGTATC
CAAGAGATGTATCGGGTAACTTCTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.135

100

0.671
  pilB Acinetobacter baylyi ADP1

65.554

100

0.656
  pilB Legionella pneumophila strain ERS1305867

52.575

98.946

0.52
  pilF Neisseria gonorrhoeae MS11

51.163

98.243

0.503
  pilB Vibrio cholerae strain A1552

49.554

98.594

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.41

99.473

0.482
  pilB Vibrio campbellii strain DS40M4

47.703

99.473

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

35.877

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.633

99.473

0.374
  pilF Thermus thermophilus HB27

36.442

100

0.371