Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LPW13_RS03245 Genome accession   NZ_CP087715
Coordinates   821173..822885 (-) Length   570 a.a.
NCBI ID   WP_230438016.1    Uniprot ID   -
Organism   Microbulbifer celer strain KCTC 12973     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 816173..827885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPW13_RS03215 (LPW13_03215) - 816473..817408 (+) 936 WP_230438010.1 hypothetical protein -
  LPW13_RS03220 (LPW13_03220) - 817453..817854 (+) 402 WP_230438011.1 hypothetical protein -
  LPW13_RS03225 (LPW13_03225) yacG 818037..818243 (-) 207 WP_230438012.1 DNA gyrase inhibitor YacG -
  LPW13_RS03230 (LPW13_03230) coaE 818255..818875 (-) 621 WP_230438013.1 dephospho-CoA kinase -
  LPW13_RS03235 (LPW13_03235) - 818933..819817 (-) 885 WP_230438014.1 A24 family peptidase -
  LPW13_RS03240 (LPW13_03240) pilC 819831..821045 (-) 1215 WP_230438015.1 type II secretion system F family protein Machinery gene
  LPW13_RS03245 (LPW13_03245) pilB 821173..822885 (-) 1713 WP_230438016.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LPW13_RS18135 - 823391..823855 (+) 465 WP_268932668.1 pilin -
  LPW13_RS03260 (LPW13_03260) - 823927..825408 (+) 1482 WP_230438017.1 O-antigen ligase -
  LPW13_RS03265 (LPW13_03265) - 825490..826911 (-) 1422 WP_230438018.1 undecaprenyl-phosphate glucose phosphotransferase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62198.98 Da        Isoelectric Point: 5.1628

>NTDB_id=631169 LPW13_RS03245 WP_230438016.1 821173..822885(-) (pilB) [Microbulbifer celer strain KCTC 12973]
MSSTPLSGLAKRLVSDNAIDEATAQSAIKAAKREGQSFAQHAVETKLIKSRELANIAANAFGTPLFDLSSYNFDLLPKDI
VAEKLISKHFALPLYKRGNRLFVAVADPTNLAGLDEFNFNTGLNTDPILVEADKLAKAIESYLANSGDIGGGLEGLDDED
LDSLDVEGGDSRPEEDGEPGGDEAPVVRFVNKVLLDAIRTGASDVHFEPYEKIYRVRMRTDGVLHEVARPPIQLATRISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKMGIDALGYEEEQKKVYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSNKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCGECKQPAQLPDEVLAEEGF
DTVTIPKSEWKIYQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQAKKEGFNNLRVSALRKVVMGVTS
LEEANRVTND

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=631169 LPW13_RS03245 WP_230438016.1 821173..822885(-) (pilB) [Microbulbifer celer strain KCTC 12973]
ATGAGCAGCACTCCACTGAGCGGCCTGGCCAAGCGGCTGGTTTCTGACAATGCCATTGACGAGGCAACGGCCCAATCTGC
CATCAAAGCGGCCAAGCGTGAGGGGCAGAGCTTTGCCCAGCATGCGGTTGAAACCAAGCTAATCAAAAGCCGAGAGCTCG
CCAACATAGCTGCCAATGCGTTTGGTACTCCGCTGTTTGACCTATCCAGCTATAACTTCGACCTGCTCCCTAAGGACATT
GTTGCTGAGAAACTGATCAGCAAGCATTTTGCCCTCCCCTTGTATAAGCGCGGGAATCGGCTGTTTGTTGCGGTCGCAGA
CCCGACCAATCTCGCGGGCCTTGATGAATTCAACTTCAATACCGGGCTGAACACTGACCCGATTCTCGTTGAAGCGGACA
AGTTAGCCAAGGCAATCGAGAGTTATCTGGCCAATAGTGGAGACATTGGCGGCGGTCTGGAAGGACTGGACGACGAAGAT
CTGGACAGCCTCGATGTAGAAGGCGGTGATAGTCGGCCTGAGGAAGACGGTGAGCCCGGTGGCGATGAAGCACCGGTAGT
GCGTTTTGTAAACAAGGTATTACTGGATGCGATCCGTACCGGCGCTTCAGATGTCCATTTCGAACCCTACGAAAAGATCT
ATCGGGTACGGATGCGCACCGATGGCGTGCTGCATGAAGTAGCGCGCCCTCCCATTCAGCTGGCCACCCGAATTTCCGCC
CGCCTCAAGGTAATGTCCAAAATGGATATTTCCGAAAGGCGTGTGCCCCAAGACGGCCGTATCAAAATGAAGCTGTCCAA
GACCAAGGCCATTGATTTTCGGGTTAACAGTCTACCTACTCTATGGGGAGAAAAGATTGTACTTCGTATTCTTGACCCTT
CTTCAGCCAAAATGGGGATCGATGCCCTGGGCTATGAAGAAGAGCAAAAGAAGGTTTATATGGATGCTCTGGCTCAACCC
CAGGGTATGATCTTGGTTACCGGACCTACCGGGTCTGGTAAAACTGTATCTCTATATACAGGCCTGAATATTCTCAATAC
TCCAGAACGCAATATCTCAACCGCGGAAGATCCAGTAGAGATCAACTTGGAGGGGATCAATCAGGTAAACGTCTCCAACA
AGGTGGGGTTGAACTTTGCCGAAGCATTGCGCTCCTTTCTCCGTCAGGATCCAGATATCGTAATGGTGGGGGAGATCCGG
GACCTGGAGACCGCAGAGATTGCGATCAAAGCTGCACAAACCGGCCACTTGGTACTGTCTACACTGCACACCAACTCAGC
ACCGGAAACCCTAACCCGATTAATGAATATGGGGGTTCCCACGTTCAATATTGCCACCTCGGTTAGTGTAATTATTGCAC
AGCGCTTGGCACGACGTCTATGTGGTGAATGTAAACAACCTGCTCAGCTTCCGGATGAAGTGTTGGCAGAAGAAGGCTTT
GATACCGTCACCATTCCCAAAAGTGAATGGAAAATCTACCAACCTGTAGGCTGTGAGCACTGCTCTAAAGGTTACAAGGG
GCGCGTGGGTGTGTATGAAGTGGTTCGCATCACTGATGGGATTTCGCGCATTATAATGGAGGGAGGCAACTCTATTCAGA
TTGCAGACCAAGCGAAAAAGGAAGGATTCAATAACCTGCGGGTGTCGGCGCTTCGCAAGGTAGTGATGGGGGTTACCAGC
CTGGAGGAGGCAAACCGGGTGACCAATGACTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.351

100

0.604
  pilB Acinetobacter baylyi ADP1

59.019

100

0.591
  pilB Legionella pneumophila strain ERS1305867

54.145

99.474

0.539
  pilB Vibrio cholerae strain A1552

50.435

100

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

52.747

95.789

0.505
  pilB Vibrio campbellii strain DS40M4

50.796

99.123

0.504
  pilF Neisseria gonorrhoeae MS11

47.619

99.474

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

37.886

100

0.409