Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5877_RS12750 Genome accession   NZ_CP104586
Coordinates   2747784..2749136 (-) Length   450 a.a.
NCBI ID   WP_003123870.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain WTJH6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2742784..2754136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5877_RS12715 (N5877_12715) thiS 2743018..2743218 (-) 201 WP_003084514.1 sulfur carrier protein ThiS -
  N5877_RS12720 (N5877_12720) - 2743321..2743698 (-) 378 WP_003084513.1 DUF423 domain-containing protein -
  N5877_RS12725 (N5877_12725) mtgA 2743735..2744466 (+) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N5877_RS12730 (N5877_12730) - 2744471..2745076 (-) 606 WP_003084511.1 hypothetical protein -
  N5877_RS12735 (N5877_12735) rpoH 2745141..2745995 (-) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  N5877_RS12740 (N5877_12740) ftsX 2746109..2747116 (-) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  N5877_RS12745 (N5877_12745) ftsE 2747116..2747787 (-) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  N5877_RS12750 (N5877_12750) pilA 2747784..2749136 (-) 1353 WP_003123870.1 signal recognition particle-docking protein FtsY Machinery gene
  N5877_RS12755 (N5877_12755) - 2749257..2750654 (+) 1398 WP_003084498.1 M16 family metallopeptidase -
  N5877_RS12760 (N5877_12760) - 2750647..2752134 (+) 1488 WP_003102862.1 M16 family metallopeptidase -
  N5877_RS12765 (N5877_12765) rsmD 2752134..2752730 (+) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  N5877_RS12770 (N5877_12770) - 2752733..2753299 (+) 567 WP_003117889.1 hypothetical protein -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 47872.27 Da        Isoelectric Point: 4.5795

>NTDB_id=631168 N5877_RS12750 WP_003123870.1 2747784..2749136(-) (pilA) [Pseudomonas aeruginosa strain WTJH6]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIG
EGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDV
AREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQ
AAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLD
GTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=631168 N5877_RS12750 WP_003123870.1 2747784..2749136(-) (pilA) [Pseudomonas aeruginosa strain WTJH6]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCGCCGG
CAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGCGCCAGCATCGGC
GAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGAC
CGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACA
GCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTC
GCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAA
GCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCT
GGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAG
GCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGA
GCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCA
CCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAACTGGAC
GGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGG
GATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(245-444)

SRP54-type protein, helical bundle domain (SRP54_N)

(160-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.454

96

0.456