Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CENROD_RS04745 Genome accession   NC_022576
Coordinates   1140268..1142016 (+) Length   582 a.a.
NCBI ID   WP_022771969.1    Uniprot ID   -
Organism   Candidatus Symbiobacter mobilis CR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1135268..1147016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CENROD_RS04720 (Cenrod_1052) rho 1135468..1136730 (-) 1263 WP_022771964.1 transcription termination factor Rho -
  CENROD_RS04730 (Cenrod_1054) trxA 1137580..1137912 (-) 333 WP_022771966.1 thioredoxin -
  CENROD_RS04735 (Cenrod_1055) - 1138144..1138791 (+) 648 WP_022771967.1 glycine zipper 2TM domain-containing protein -
  CENROD_RS04740 (Cenrod_1056) - 1138822..1140159 (-) 1338 WP_022771968.1 multifunctional CCA addition/repair protein -
  CENROD_RS04745 (Cenrod_1057) pilF 1140268..1142016 (+) 1749 WP_022771969.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CENROD_RS04750 (Cenrod_1058) pilC 1142163..1143389 (+) 1227 WP_022771970.1 type II secretion system F family protein Machinery gene
  CENROD_RS04755 (Cenrod_1059) - 1143389..1144297 (+) 909 WP_022771971.1 prepilin peptidase -
  CENROD_RS04760 (Cenrod_1060) coaE 1144353..1145015 (+) 663 WP_022771972.1 dephospho-CoA kinase -
  CENROD_RS04765 (Cenrod_1061) zapD 1145050..1145829 (+) 780 WP_022771973.1 cell division protein ZapD -
  CENROD_RS13030 (Cenrod_1062) - 1145884..1146123 (+) 240 WP_022771974.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63582.12 Da        Isoelectric Point: 6.5775

>NTDB_id=62857 CENROD_RS04745 WP_022771969.1 1140268..1142016(+) (pilF) [Candidatus Symbiobacter mobilis CR]
MVASESPTADAPHAASFGELPGVANALIRAGRIQPSRAQEIHRKALARKVPFTAELLGSGVLTPSELAHFLCVAFSLPMV
DLDAVDPTRIIRDALDTRLCQEQRIVVLGRRHNRLVIATADPTDKQMLEKVKFATRQNADWVIAECDKLHKFIAANLAST
VDTIESLVGDEIEFEDMVSEAATDDASSTTSTEVDEAPVVRFLQKMLFDAFSMRASDLHFEPYEHTYRVRFRIDGELREI
ANPPIAIKDKLASRIKVISRLDISEKRVPQDGKVRLRLSADKVVDFRVSTLPTLFGEKIVIRILDPSSARLGVDALGYGA
IEKQRLLSAIQRPHGMILVTGPTGAGKTVSLYTCLNLLNQPGVNIATAEDPAEIQLPGINQVSINDKAGLTFSVALKAFL
RQDPDIIMVGEIRDRDTADIAIKAAQTGHLVLSTLHTNDAPATLVRMRNMGVEPFNIASSVILITAQRLARRLCPRCKQP
AEVPSSALLSAGFKAEDLDGSWKPYRSVGCNACHNGYKGRVGLYQVMPITEEMQRIILYDGSALDIAAQAQYDGVATLRE
AGLVKVREGLTSLEEVLSVTNP

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=62857 CENROD_RS04745 WP_022771969.1 1140268..1142016(+) (pilF) [Candidatus Symbiobacter mobilis CR]
ATGGTTGCCTCTGAGTCCCCCACTGCCGACGCTCCCCATGCTGCTTCTTTCGGGGAACTTCCCGGCGTAGCCAATGCCTT
GATTCGTGCAGGGCGCATCCAACCATCGCGAGCGCAGGAAATACACCGCAAAGCCTTGGCACGCAAAGTGCCGTTCACTG
CGGAACTTCTCGGAAGCGGGGTGTTGACCCCTTCCGAACTGGCGCATTTCCTGTGTGTGGCGTTCTCTCTGCCTATGGTC
GACCTCGATGCCGTCGACCCCACCCGCATCATCCGTGACGCCCTTGATACCCGACTGTGCCAGGAACAGCGTATCGTCGT
CCTCGGCAGACGGCATAACCGCCTCGTCATCGCGACTGCCGACCCGACCGACAAACAAATGCTGGAAAAGGTCAAATTTG
CCACCCGGCAAAACGCCGACTGGGTGATTGCCGAATGCGACAAGCTCCACAAGTTCATCGCGGCGAACCTGGCTTCGACG
GTAGACACCATCGAGAGCCTGGTAGGGGACGAGATCGAGTTCGAGGACATGGTGTCCGAGGCCGCCACCGACGATGCATC
CTCCACTACCAGTACCGAGGTCGACGAAGCGCCCGTGGTGCGCTTTTTGCAAAAGATGCTCTTCGACGCGTTTTCGATGC
GCGCTTCCGACCTGCACTTCGAACCCTACGAGCACACCTACCGGGTACGTTTCCGCATTGATGGGGAGTTGCGCGAAATC
GCCAATCCGCCGATCGCTATCAAGGACAAGCTCGCTTCGCGCATCAAAGTCATTTCCCGGCTCGACATTTCCGAAAAGCG
CGTCCCGCAAGACGGCAAGGTGCGCCTGCGCCTCAGTGCAGACAAGGTCGTGGACTTTCGCGTGAGCACCCTGCCAACGT
TGTTCGGCGAAAAAATCGTTATCCGGATTCTCGACCCCAGCAGTGCCAGGTTGGGCGTCGATGCGCTCGGGTACGGGGCG
ATCGAAAAACAGCGTCTGCTCTCGGCCATCCAGCGCCCGCACGGGATGATCCTCGTCACCGGGCCTACCGGCGCGGGCAA
GACCGTCTCGCTCTACACCTGCCTGAACCTGCTCAATCAACCCGGGGTCAACATCGCCACGGCAGAAGACCCTGCGGAAA
TCCAGCTTCCCGGCATCAACCAGGTTTCGATCAACGACAAAGCAGGGTTGACATTCTCCGTCGCGCTCAAGGCTTTCCTG
CGCCAGGATCCAGACATCATCATGGTCGGCGAAATTCGCGACCGTGACACAGCGGACATCGCCATCAAGGCCGCGCAGAC
TGGGCACCTGGTGCTCTCCACGCTGCATACGAACGATGCCCCGGCCACCCTCGTACGGATGCGCAACATGGGCGTAGAGC
CTTTCAACATCGCTTCCAGCGTGATTTTGATTACCGCCCAACGGCTGGCGCGGCGGCTATGCCCCAGATGCAAGCAGCCT
GCAGAGGTTCCCTCCAGCGCATTGTTAAGCGCTGGATTCAAGGCCGAAGATCTGGACGGTAGCTGGAAACCGTACCGCTC
CGTGGGGTGCAACGCCTGTCACAACGGCTACAAAGGCCGCGTTGGCCTGTACCAGGTTATGCCCATCACCGAAGAGATGC
AACGTATCATCCTTTACGACGGTAGTGCGCTGGACATCGCCGCGCAGGCGCAATACGACGGTGTCGCGACGCTGCGAGAG
GCTGGCTTGGTCAAGGTGCGGGAAGGACTGACCTCGTTGGAAGAAGTGCTGTCGGTCACAAACCCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(205-472)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(58-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.442

90.893

0.495
  pilB Acinetobacter baylyi ADP1

49.823

96.907

0.483
  pilB Acinetobacter baumannii D1279779

53.448

89.691

0.479
  pilB Legionella pneumophila strain ERS1305867

48.05

96.907

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

44.991

97.766

0.44
  pilB Vibrio campbellii strain DS40M4

43.972

96.907

0.426
  pilB Vibrio cholerae strain A1552

46.768

90.378

0.423
  pilF Thermus thermophilus HB27

37.696

98.454

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.179

92.096

0.361

Multiple sequence alignment