Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY67_RS23425 Genome accession   NZ_CP087203
Coordinates   5386888..5388588 (+) Length   566 a.a.
NCBI ID   WP_265064643.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-056     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5381888..5393588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY67_RS23405 (LOY67_23380) - 5382011..5384944 (+) 2934 WP_265064639.1 DUF748 domain-containing protein -
  LOY67_RS23410 (LOY67_23385) - 5384946..5385242 (+) 297 WP_265064640.1 DUF2845 domain-containing protein -
  LOY67_RS23415 (LOY67_23390) - 5385545..5385898 (-) 354 WP_265064641.1 BON domain-containing protein -
  LOY67_RS23420 (LOY67_23395) - 5386254..5386664 (-) 411 WP_265064642.1 pilin -
  LOY67_RS23425 (LOY67_23400) pilB 5386888..5388588 (+) 1701 WP_265064643.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY67_RS23430 (LOY67_23405) pilC 5388591..5389808 (+) 1218 WP_265064644.1 type II secretion system F family protein Machinery gene
  LOY67_RS23435 (LOY67_23410) pilD 5389810..5390679 (+) 870 WP_265064645.1 prepilin peptidase Machinery gene
  LOY67_RS23440 (LOY67_23415) coaE 5390690..5391313 (+) 624 WP_265064646.1 dephospho-CoA kinase -
  LOY67_RS23445 (LOY67_23420) yacG 5391310..5391516 (+) 207 WP_265064647.1 DNA gyrase inhibitor YacG -
  LOY67_RS23450 (LOY67_23425) - 5391564..5392250 (-) 687 WP_265064648.1 energy-coupling factor ABC transporter permease -
  LOY67_RS23455 (LOY67_23430) - 5392579..5393205 (+) 627 WP_024779575.1 DUF1780 domain-containing protein -
  LOY67_RS23460 (LOY67_23435) - 5393307..5393480 (+) 174 WP_003185543.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62716.61 Da        Isoelectric Point: 5.2273

>NTDB_id=628067 LOY67_RS23425 WP_265064643.1 5386888..5388588(+) (pilB) [Pseudomonas sp. B21-056]
MNDITLSGLTKQLVLAELITEQSAQQAQQQAQRNRMPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKESQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSTTGLEGMGDVDLDG
LDIESDDDRKQDAITGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRVRTDGMLREVARPPTQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYLAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNQCKKATEIPEETLLKEGFPK
ERIGSFTIYEPVGCEQCNSGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=628067 LOY67_RS23425 WP_265064643.1 5386888..5388588(+) (pilB) [Pseudomonas sp. B21-056]
ATGAATGACATCACCCTCAGCGGTTTGACCAAGCAACTGGTGCTGGCCGAGCTGATCACCGAGCAAAGCGCGCAGCAGGC
GCAGCAGCAAGCCCAACGCAATCGCATGCCCCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCCAGAGCCGCCAGGTTG
CGGAAATCGCCTCGGAGCATTTTGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAAGCCAGCCCACTGGCCTT
GTCAGTGAGAAACTGGTCCGGCAACACCATGCCCTGCCGCTGTGGCGGCGCGGTAACAAGCTGTTCGTGGGGATCTCCGA
CCCCACCAACCACCAGGCCATCAACGACATCCAGTTCAGTACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGATGACA
AACTCAGCGATGCCATCGAGAAGTTTTTCGAATCCAGCACCACGGGCCTGGAAGGCATGGGTGATGTCGACCTCGATGGC
CTGGACATCGAGTCGGACGACGATCGCAAGCAGGACGCCATCACCGGCCAGGACACCGATGACGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCGGACCTGCACTTCGAACCCTATGAAAAATCCTATCGGG
TGCGGGTGCGTACCGACGGCATGCTGCGGGAAGTGGCCAGGCCGCCGACCCAACTGGCCACCCGCATCGCCGCGCGCCTG
AAGGTCATGGCCAGCCTGGATATTTCCGAGCGGCGTAAACCGCAGGACGGCCGGCTGAAGATGCGCCTGTCGAAAACCAA
ATCCATCGACTTCCGGGTCAATACCCTGCCCACGCTCTGGGGCGAAAAGGTGGTGATCCGGATCCTCGACCCTTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAGCCGGATCAGAAAGACCTCTATCTCGCGGCCCTCAAGCAACCACAAGGG
CTGATCCTGGTGACCGGACCGACCGGCTCGGGCAAGACTGTCTCGCTCTACACCGGCTTGAATATCCTCAATACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAATCAGGTCAACGTGAACCCACGTCAGG
GAATGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGATGTGATCATGGTGGGTGAGATCCGCGACCTG
GAAACGGCTGAAATTGCCATCAAGGCAGCCCAGACCGGGCACATGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTAATCCGCTTGCAGAACATGGGCATCCCCGGCTTCAACATCGCCACGGCGGTCCACCTGATCATCGCCCAGCGTC
TGGCGCGAAAACTGTGCAACCAATGCAAGAAAGCCACGGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGCTTCCCCAAG
GAACGCATCGGCTCATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAGCGGCTACAAGGGACGCGTGGGGGT
TTATGAAGTGGTGAAAAATACGCCTGAGCTGCAACGGCTGATCATGGCCGAAGGCAATTCCCTGGAAATCGATCTGCAGA
TGCGCAAGGACGGCTTCAATGACCTGCGCACTTCGGGGCTGCTCAAAGTGATGCAAGGCGTGACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.979

99.647

0.578
  pilB Acinetobacter baumannii D1279779

57.672

100

0.578
  pilB Legionella pneumophila strain ERS1305867

55.026

100

0.551
  pilB Vibrio cholerae strain A1552

50.353

100

0.504
  pilB Vibrio campbellii strain DS40M4

48.319

99.823

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.002

97.35

0.477
  pilF Neisseria gonorrhoeae MS11

47.957

99.47

0.477