Detailed information NTG0628039

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY63_RS15550
• Genome accession: NZ_CP087202
• Coordinates: 3586861..3588027 (-)
• Length: 388 a.a.
• NCBI ID: WP_010451351.1
• Uniprot ID: A0A1H1XWA0
• Organism: Pseudomonas asplenii strain B21-058
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3581861..3593027

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY63_RS15535 (LOY63_15525)	-	3582677..3584155 (+)	1479	WP_265084109.1	mannitol dehydrogenase family protein	-
LOY63_RS15540 (LOY63_15530)	xylB	3584335..3585825 (+)	1491	WP_265084110.1	xylulokinase	-
LOY63_RS15545 (LOY63_15535)	-	3585832..3586770 (+)	939	WP_010451352.1	carbohydrate kinase	-
LOY63_RS15550 (LOY63_15540)	pilU	3586861..3588027 (-)	1167	WP_010451351.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY63_RS15555 (LOY63_15545)	-	3588163..3590697 (+)	2535	WP_265084111.1	PAS domain-containing protein	-
LOY63_RS15560 (LOY63_15550)	-	3590756..3591031 (+)	276	WP_007932676.1	peptidylprolyl isomerase	-
LOY63_RS15565 (LOY63_15555)	-	3591019..3591951 (-)	933	WP_265084112.1	sugar kinase	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 42995.19 Da
• Isoelectric Point: 6.1925

>NTDB_id=628039 LOY63_RS15550 WP_010451351.1 3586861..3588027(-) (pilU) [Pseudomonas asplenii strain B21-058]
MEIHALLKLLAAQDGSDLYLSTGAPPCAKFNGVLKPLGTDALKAGEVALIADGIMDAEQRQQFERELEMNLALSLPGVGR
FRVNIFKQRNEVSIVARNIKLEIPRFEDLKLPPILLDTIMEKRGLVLFVGATGSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLQAFVSQRLVRTTDGKRRAAVEVMLGTPTVRDFIKRNEFSELKGIMEKSEVSGMLTFDTAL
FNLVVEGAIDEDEALKNADSVNNLRLRLKLHQDGAAIQQKTPPAPPVAAQELDTKDWGLVDDIDTPAQ

Nucleotide

• Length: 1167 bp

>NTDB_id=628039 LOY63_RS15550 WP_010451351.1 3586861..3588027(-) (pilU) [Pseudomonas asplenii strain B21-058]
ATGGAAATCCACGCCTTATTGAAACTCCTTGCCGCCCAGGACGGCTCGGATCTTTACCTGTCGACCGGCGCACCGCCCTG
TGCCAAGTTCAATGGCGTGCTCAAGCCGCTGGGCACCGACGCACTCAAGGCCGGCGAGGTGGCGCTGATTGCCGACGGTA
TCATGGACGCCGAACAGCGCCAGCAGTTCGAACGCGAGCTGGAAATGAACCTGGCGTTGTCATTGCCGGGCGTCGGGCGT
TTTCGGGTGAATATTTTCAAGCAACGCAACGAAGTCTCCATCGTTGCCCGCAATATCAAGCTGGAGATCCCTCGTTTCGA
AGACCTCAAGCTGCCGCCGATCCTGCTCGACACCATCATGGAAAAGCGCGGCCTGGTGCTCTTCGTCGGTGCTACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGGCACAGCAGTGGCCATATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAAGTGGGGGTCGACACCCGCAGTTTCCATGC
GGCGCTGAAAAATACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTTTTCCCGGAAGACCGCCGCCCGCAGTTGCTCAACGACCTGGGCAACAACCTGCAGGCCTTCGTCTCCCAACG
GCTGGTCCGTACCACCGATGGCAAGCGCCGGGCGGCGGTCGAGGTGATGCTGGGGACGCCGACCGTTCGCGACTTCATCA
AGCGCAACGAGTTCTCTGAACTCAAGGGCATCATGGAGAAGTCCGAAGTCAGCGGCATGCTGACGTTCGATACGGCGCTG
TTCAACCTGGTGGTGGAGGGCGCGATCGACGAGGACGAGGCACTGAAGAATGCCGACTCGGTGAACAACCTGCGTCTGCG
GCTGAAATTGCACCAGGACGGCGCGGCGATCCAGCAGAAAACCCCACCGGCCCCTCCCGTGGCGGCACAGGAGCTGGATA
CCAAGGATTGGGGCCTGGTCGACGACATCGATACACCGGCTCAGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-271)

3D structure

• AlphaFold DB: A0A1H1XWA0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.98	90.464	0.515
pilU	Acinetobacter baylyi ADP1	52.809	91.753	0.485
pilU	Vibrio cholerae strain A1552	50.571	90.206	0.456
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40	95.361	0.381
pilT	Pseudomonas stutzeri DSM 10701	41.159	88.918	0.366
pilT	Pseudomonas aeruginosa PAK	40.58	88.918	0.361