Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY66_RS02100 Genome accession   NZ_CP087200
Coordinates   475127..477193 (+) Length   688 a.a.
NCBI ID   WP_135843314.1    Uniprot ID   -
Organism   Pseudomonas viciae strain B21-062     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470127..482193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY66_RS02080 (LOY66_02075) comM 472339..473403 (+) 1065 WP_135843310.1 pilus assembly protein PilM Machinery gene
  LOY66_RS02085 (LOY66_02080) - 473403..473969 (+) 567 WP_135843311.1 PilN domain-containing protein -
  LOY66_RS02090 (LOY66_02085) pilO 473966..474589 (+) 624 WP_135843312.1 type 4a pilus biogenesis protein PilO -
  LOY66_RS02095 (LOY66_02090) - 474586..475113 (+) 528 WP_135843313.1 pilus assembly protein PilP -
  LOY66_RS02100 (LOY66_02095) pilQ 475127..477193 (+) 2067 WP_135843314.1 type IV pilus secretin PilQ Machinery gene
  LOY66_RS02105 (LOY66_02100) aroK 477198..477716 (+) 519 WP_123345370.1 shikimate kinase AroK -
  LOY66_RS02110 (LOY66_02105) aroB 477864..478964 (+) 1101 WP_256454108.1 3-dehydroquinate synthase -
  LOY66_RS02115 (LOY66_02110) - 478975..480537 (+) 1563 WP_265072614.1 AAA family ATPase -

Sequence

Protein


Download         Length: 688 a.a.        Molecular weight: 74425.59 Da        Isoelectric Point: 5.2919

>NTDB_id=627972 LOY66_RS02100 WP_135843314.1 475127..477193(+) (pilQ) [Pseudomonas viciae strain B21-062]
MNRIFSVLGISLWIALLSPMVHAASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTDQPARIALDLPGVTNQLASKRRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGVAPKTQAARPAVASAAPARASAPVGKAIRGVDFQ
RGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAIAGGDRATISIEPSGTFDYS
TYQTDNKLTVSVRPMTVEDLQKRNSDRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKADER
GTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWSSSGVTTGDST
TIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEAS
SSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQSK
VVDKVPFLGDVPYLGRLFRRDVVSETKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2067 bp        

>NTDB_id=627972 LOY66_RS02100 WP_135843314.1 475127..477193(+) (pilQ) [Pseudomonas viciae strain B21-062]
ATGAACAGGATTTTTTCAGTCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCATGCGGCCAGCCTGAA
GACGCTGGATGTCGCGGCGTTGCCGGGTGATCGCGTCGAGTTGAAACTCACCTTCGACGGGCCACCGCCGACGCCCCGGG
GCTACACCACCGATCAACCGGCCCGGATCGCGCTGGACCTGCCTGGCGTGACCAATCAACTGGCCAGCAAGCGCCGTGAT
CTGGGCGGTGGCAATGCGCGTAGCGCCACCGTGGTCGAGGCGGGCGACCGGACGCGGCTGATCATCGGCCTTACCCAACT
GGCGCCATATGACACCCGGATCGAAGGCAACAACCTGTTCGTGGTGGTTGGCAAAGGCGTCGCGCCCAAGACCCAGGCTG
CGCGTCCGGCGGTCGCCAGTGCCGCACCTGCCAGGGCCAGTGCGCCGGTGGGCAAGGCTATCCGTGGCGTGGATTTCCAG
CGCGGAACCCAGGGTGAAGGCAATGTGGTGATCGATCTGTCGGACCCTTCCATCGCTCCGGACATCCAGGAGCGTGAAGG
CAAGATCATCCTCAATTTCGCCAGGACCCAGTTGCCCGAGCCGCTGCGGGTGCGCCTGGACGTCAAGGACTTCGCCACCC
CGGTGCAGTTCGTCAATGCCATTGCCGGCGGCGACCGGGCGACCATCAGCATCGAACCCAGCGGCACCTTCGATTATTCC
ACCTACCAGACCGACAACAAGCTGACCGTCAGCGTCCGGCCGATGACGGTCGAGGACCTGCAAAAACGCAATTCCGATCG
CGCCGCCTACAGCGGTGAGAAACTCTCCCTCAACTTTCAGGACATCGAAGTGCGTTCGGTCCTGCAACTGATCGCCGATT
TCACCAACCTGAACCTGGTGGCCAGCGATACGGTGCAGGGCGGTATCACCCTGCGCTTGCAGAATGTGCCTTGGGACCAG
GCGCTGGATCTGGTGCTCAAGACCAAAGGGCTGGACAAGCGCAAGGTCGGTAACGTGTTGCTGGTGGCGCCGGCCGATGA
AATTGCGGCCCGCGAACGCCAGGAGCTGGAGTCCCAGAAGCAGATCGCCGACCTGGCGCCGCTGCGGCGTGAGCTGTTGC
AGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCGAAAGCCGATGAGCGC
GGCACGATCACGGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCGACTCGACGAGCTACGCCGGAT
CGTGGCGCAGCTGGATATTCCGGTGCGTCAAGTGATGATCGAGGCGCGGATCGTCGAGGCCAATGTCGATTACGACAAAA
GCCTGGGTGTGCGTTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAGCTCCTCCGGGGTCACCACCGGCGACTCCACC
ACCATTGGCACGCCGGGCAGCACCAGCACCAACCAGCCGTTTGTCGATCTGGGCGCGTCGGGCAACACCTCCGGAATCGG
CATTGCGTTCATCACCGACAACGTGTTGCTGGATCTGGAACTGACGGCCATGGAGAAAACCGGCAACGGCGAAATCGTCT
CCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCCAAAATCCTCAAGGGCACGGAAATCCCCTATCAGGAAGCCAGC
TCCAGCGGTGCCACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTGACGCCGCAGATCACCCCGGACAACCG
CATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAAGTGCAGGATGTGCCGCCGATCAAGAAAA
ACGAGGTCAACGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACTCAGAGCAAG
GTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGAGACAAA
ATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II and III secretion system protein (Secretin)

(523-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.007

100

0.759
  comQ Acinetobacter baylyi ADP1

41.655

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.347

100

0.411
  pilQ Acinetobacter baumannii D1279779

38.347

100

0.411
  pilQ Legionella pneumophila strain Lp02

38.244

97.674

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.266

97.238

0.372