Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY43_RS24180 Genome accession   NZ_CP087175
Coordinates   5444872..5446572 (+) Length   566 a.a.
NCBI ID   WP_095048681.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-041     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5439872..5451572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY43_RS24160 (LOY43_24125) - 5440031..5442979 (+) 2949 WP_258708830.1 DUF748 domain-containing protein -
  LOY43_RS24165 (LOY43_24130) - 5442979..5443275 (+) 297 WP_258708831.1 DUF2845 domain-containing protein -
  LOY43_RS24170 (LOY43_24135) - 5443563..5443919 (-) 357 WP_047600285.1 BON domain-containing protein -
  LOY43_RS24175 (LOY43_24140) - 5444214..5444645 (-) 432 WP_095048680.1 pilin -
  LOY43_RS24180 (LOY43_24145) pilB 5444872..5446572 (+) 1701 WP_095048681.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY43_RS24185 (LOY43_24150) pilC 5446575..5447792 (+) 1218 WP_093434875.1 type II secretion system F family protein Machinery gene
  LOY43_RS24190 (LOY43_24155) pilD 5447794..5448666 (+) 873 WP_095048682.1 A24 family peptidase Machinery gene
  LOY43_RS24195 (LOY43_24160) coaE 5448663..5449286 (+) 624 WP_258708832.1 dephospho-CoA kinase -
  LOY43_RS24200 (LOY43_24165) yacG 5449283..5449483 (+) 201 WP_095048684.1 DNA gyrase inhibitor YacG -
  LOY43_RS24205 (LOY43_24170) - 5449490..5449705 (-) 216 WP_007909901.1 hypothetical protein -
  LOY43_RS24210 (LOY43_24175) - 5449771..5450460 (-) 690 WP_095048686.1 energy-coupling factor ABC transporter permease -
  LOY43_RS24215 (LOY43_24180) - 5450457..5450924 (-) 468 WP_095048687.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62640.61 Da        Isoelectric Point: 5.9184

>NTDB_id=627331 LOY43_RS24180 WP_095048681.1 5444872..5446572(+) (pilB) [Pseudomonas sp. B21-041]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHAMPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHTSGLEDMADVDLDG
VDVESVDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=627331 LOY43_RS24180 WP_095048681.1 5444872..5446572(+) (pilB) [Pseudomonas sp. B21-041]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
TTGGCAACAGGCCCAACGCAATCGTCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTCACAGGTCG
CCGAGATCGCCTCCGAGCATTTCGGCATGGCCTTGCTTGATCTCAACTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTCGTGCGTCAGCATCACGCCATGCCCTTGTGGCGTCGCGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAAGCAATCAACGACATCCAGTTCAGTACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AACTTACCGATGCCATCGAGAAATTCTTCGACACCCACACCAGCGGTCTGGAGGACATGGCCGATGTCGATCTCGACGGG
GTCGATGTCGAATCCGTCGACGACAGCAAGCAGGACGCCATCGGCGGGCTCGACGCCGACGATGCGCCGGTGGTGCGCTT
CGTGCACAAGATGTTGCTGGATGCGATCAAGAGTGGCTCTTCCGACTTGCACTTCGAGCCTTACGAAAAAAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTTGCCAAACCGCCGATTCAATTGGCCGGGCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCGGAACGGCGCAAACCTCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
GTCGATCGATTTCCGGGTCAACACGCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGTATCGACGCGCTCGGCTATGAGCCAGAGCAGAAAGACCTGTACATGGCCGCACTCAAGCAGCCACAGGGC
ATGATTCTGGTCACCGGGCCGACCGGTTCGGGTAAAACCGTTTCGCTGTATACCGGCCTGAACATCCTCAACACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GGCTCGACTTTGCCCAGGCACTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGTGATCTG
GAAACTGCCGAGATTGCGATCAAGGCTGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCTGCGGA
AACCCTGACGCGCCTGCACAACATGGGTATTCCCGGTTTCAACATCGCTACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCACTGCAAAAAAGCCATCGAGATCCCGCGCGAAACCTTACTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGCTTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACCTGCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCCGGACTGCACAAGGCCATGCAAGGCATCACCAGTCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.47

0.588
  pilB Acinetobacter baumannii D1279779

58.156

99.647

0.58
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilF Neisseria gonorrhoeae MS11

49.911

99.47

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.47

0.496
  pilB Vibrio campbellii strain DS40M4

48.09

100

0.489
  pilF Thermus thermophilus HB27

36.918

98.587

0.364