Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LOY43_RS15635 Genome accession   NZ_CP087175
Coordinates   3519441..3520550 (-) Length   369 a.a.
NCBI ID   WP_095047807.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-041     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3514441..3525550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY43_RS15630 (LOY43_15595) - 3515545..3519312 (-) 3768 WP_258708329.1 hypothetical protein -
  LOY43_RS15635 (LOY43_15600) pilU 3519441..3520550 (-) 1110 WP_095047807.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LOY43_RS15640 (LOY43_15605) - 3520664..3523201 (+) 2538 WP_220556345.1 PAS domain S-box protein -
  LOY43_RS15645 (LOY43_15610) - 3523253..3523528 (+) 276 WP_007912322.1 peptidylprolyl isomerase -
  LOY43_RS15650 (LOY43_15615) - 3523659..3524594 (-) 936 WP_258708330.1 sugar kinase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40871.68 Da        Isoelectric Point: 6.5829

>NTDB_id=627326 LOY43_RS15635 WP_095047807.1 3519441..3520550(-) (pilU) [Pseudomonas sp. B21-041]
MEIDALLSILSNKNGSDLFLSTGAPPSARIDGVLTVLSERPFKNGETAAIGASLMDAEQRREFDRDLEMNLAISRAGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFADLKLPAVLLDTVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHAHNANQALDRI
INMFPEERRPQLLHTLGNNLKAFISQRLVRTVDGQRRAAVEVLLGTPTIADLVRRGQFEELKPIMEKSTELGMQTFDAAL
YALVAEGAISAQEALKNADSVNNLKLRMKLSEEANAGNDTTSEEWGLID

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=627326 LOY43_RS15635 WP_095047807.1 3519441..3520550(-) (pilU) [Pseudomonas sp. B21-041]
ATGGAAATCGATGCGCTGTTGTCAATTTTGTCCAATAAAAACGGTTCGGACCTGTTCCTCTCCACGGGAGCACCGCCCAG
TGCGCGCATTGACGGCGTGCTCACAGTGTTGAGCGAACGCCCGTTCAAAAACGGTGAAACCGCGGCCATCGGCGCCTCCC
TGATGGACGCCGAGCAACGACGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCGCGAGCGGGCATCGGGCGT
TTTCGCGTCAATATCTTTAAGCAGCGCAATGATGTATCGATCGTCATTCGCAATGTCAAACTCGACATCCCGCGTTTTGC
CGATCTGAAATTGCCGGCAGTACTGCTTGACACCGTCATGCTCAAGCAGGGCTTGATCCTCTTTGTCGGGGCCACCGATT
CGGGGAAGTCGACCTCGCTGGCCGCGCTGATCGACCACCGCAACCGCCATAGCAGCGGGCACATCATCACCATCGAGGAC
CCGATCGAGTACATCCATCGCCACCAGCGCTCGATCATCAATCAACGGGAAGTCGGGGTCGATACACGCAGCTTCCACGC
GGCCCTGAAAAACACCCTGCGCCAGGCACCGGACGTGGTGCTGATCGGCGAAATCCGTGACCGCGAAACCATGGAACATG
CCCTGGCGTTCGCCGATACCGGGCATCTGGTGCTCTCGACGTTGCACGCGCACAACGCCAATCAGGCACTGGACCGAATT
ATCAATATGTTCCCGGAAGAACGGCGACCGCAGTTGTTGCATACGCTGGGAAACAACCTGAAAGCATTTATCTCGCAACG
CTTAGTGCGCACCGTCGATGGGCAACGCAGGGCAGCTGTCGAGGTGCTGTTGGGCACGCCAACCATCGCAGATCTGGTTC
GGCGCGGACAGTTCGAGGAGCTGAAACCGATAATGGAAAAATCCACCGAACTGGGTATGCAGACATTTGATGCGGCGCTG
TACGCATTAGTCGCTGAAGGTGCAATCAGTGCGCAGGAAGCTTTAAAGAATGCTGATTCGGTGAATAATTTGAAGTTGCG
GATGAAACTTTCTGAAGAGGCAAATGCTGGTAACGATACAACGTCCGAAGAATGGGGGTTGATCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(69-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.707

99.729

0.556
  pilU Acinetobacter baylyi ADP1

54.062

96.748

0.523
  pilU Vibrio cholerae strain A1552

50.139

97.29

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.385

97.019

0.382
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90.244

0.366
  pilT Vibrio cholerae strain A1552

40.541

90.244

0.366