Detailed information NTG0626876

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LOK79_RS12260
• Genome accession: NZ_CP087137
• Coordinates: 2524742..2525356 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JIN4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2519742..2530356

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOK79_RS12230 (LOK79_12230)	rpsT	2520042..2520308 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LOK79_RS12235 (LOK79_12235)	holA	2520325..2521366 (-)	1042	Protein_2366	DNA polymerase III subunit delta	-
LOK79_RS12240 (LOK79_12240)	-	2521406..2521560 (-)	155	Protein_2367	hypothetical protein	-
LOK79_RS12245 (LOK79_12245)	-	2521601..2521735 (+)	135	WP_003152870.1	YqzM family protein	-
LOK79_RS12250 (LOK79_12250)	comEC	2521754..2524105 (-)	2352	WP_058906194.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LOK79_RS12255 (LOK79_12255)	-	2524106..2524675 (-)	570	WP_003152868.1	ComE operon protein 2	-
LOK79_RS12260 (LOK79_12260)	comEA	2524742..2525356 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
LOK79_RS12265 (LOK79_12265)	comER	2525415..2526236 (+)	822	WP_014305449.1	late competence protein ComER	-
LOK79_RS12270 (LOK79_12270)	-	2526305..2527042 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
LOK79_RS12275 (LOK79_12275)	rsfS	2527039..2527395 (-)	357	WP_003152864.1	ribosome silencing factor	-
LOK79_RS12280 (LOK79_12280)	yqeK	2527392..2527973 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LOK79_RS12285 (LOK79_12285)	-	2527963..2528532 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
LOK79_RS12290 (LOK79_12290)	yhbY	2528543..2528833 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LOK79_RS12295 (LOK79_12295)	aroE	2528827..2529669 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=626876 LOK79_RS12260 WP_003152867.1 2524742..2525356(-) (comEA) [Bacillus velezensis strain JIN4]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=626876 LOK79_RS12260 WP_003152867.1 2524742..2525356(-) (comEA) [Bacillus velezensis strain JIN4]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363