Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VIBNI_RS00885 Genome accession   NC_022528
Coordinates   176203..176661 (-) Length   152 a.a.
NCBI ID   WP_022601072.1    Uniprot ID   -
Organism   Vibrio nigripulchritudo     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 171203..181661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBNI_RS00865 (VIBNI_A0172) coaE 171708..172313 (-) 606 WP_022549574.1 dephospho-CoA kinase -
  VIBNI_RS00870 (VIBNI_A0173) pilD 172363..173232 (-) 870 WP_022549575.1 A24 family peptidase Machinery gene
  VIBNI_RS00875 (VIBNI_A0174) pilC 173273..174505 (-) 1233 WP_022549576.1 type II secretion system F family protein Machinery gene
  VIBNI_RS00880 (VIBNI_A0175) pilB 174515..176203 (-) 1689 WP_022549577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VIBNI_RS00885 (VIBNI_A0176) pilA 176203..176661 (-) 459 WP_022601072.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VIBNI_RS00890 (VIBNI_A0177) nadC 176939..177826 (-) 888 WP_022549579.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VIBNI_RS00895 (VIBNI_A0178) ampD 177955..178512 (+) 558 WP_022549580.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VIBNI_RS00900 (VIBNI_A0180) pdhR 178825..179595 (+) 771 WP_004397664.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15773.16 Da        Isoelectric Point: 7.6647

>NTDB_id=62565 VIBNI_RS00885 WP_022601072.1 176203..176661(-) (pilA) [Vibrio nigripulchritudo]
MKNKKQQGFTLIELMIVVAIVAILSAFAVPAYQNYTKKATLAEFPKVAAAMKLAVELCAHENASNATTFKSNCTSAGSHV
PAKITNLNDISIEAVGATSGGINIIAKAVAVKGPIAVNEEYIMNASYAAAGLTWTASCKDGSGAAQTDYCPD

Nucleotide


Download         Length: 459 bp        

>NTDB_id=62565 VIBNI_RS00885 WP_022601072.1 176203..176661(-) (pilA) [Vibrio nigripulchritudo]
ATGAAAAATAAAAAACAACAGGGCTTTACACTGATAGAACTAATGATTGTTGTGGCGATCGTTGCAATTCTTTCAGCTTT
TGCCGTTCCTGCTTACCAAAACTATACAAAGAAAGCGACATTGGCAGAGTTTCCAAAAGTTGCTGCTGCAATGAAACTCG
CTGTTGAACTGTGTGCTCATGAAAATGCTTCAAACGCAACTACCTTTAAGTCCAACTGTACATCTGCCGGTTCTCACGTC
CCAGCGAAAATCACTAACTTAAACGACATTTCAATTGAAGCAGTTGGTGCAACAAGTGGAGGTATTAACATTATTGCCAA
AGCTGTGGCTGTAAAAGGACCTATCGCTGTCAATGAAGAATACATAATGAATGCATCTTATGCTGCTGCAGGTTTGACTT
GGACAGCAAGCTGTAAAGATGGATCTGGTGCAGCACAAACCGACTATTGCCCTGATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.94

99.342

0.586
  pilA Haemophilus influenzae 86-028NP

44.118

89.474

0.395
  pilA Haemophilus influenzae Rd KW20

44.444

88.816

0.395
  pilA2 Legionella pneumophila strain ERS1305867

41.727

91.447

0.382
  pilA2 Legionella pneumophila str. Paris

41.007

91.447

0.375

Multiple sequence alignment