Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KT882_RS07280 Genome accession   NZ_CP086370
Coordinates   1648453..1650183 (-) Length   576 a.a.
NCBI ID   WP_161072601.1    Uniprot ID   A0A7X4HBN5
Organism   Pseudoduganella aquatica strain HC52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1643453..1655183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KT882_RS07265 - 1645026..1646417 (+) 1392 WP_161072604.1 GntP family permease -
  KT882_RS07270 - 1646428..1647207 (+) 780 WP_161072603.1 3-hydroxybutyrate dehydrogenase -
  KT882_RS07275 pilC 1647219..1648442 (-) 1224 WP_161072602.1 type II secretion system F family protein Machinery gene
  KT882_RS07280 pilF 1648453..1650183 (-) 1731 WP_161072601.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KT882_RS07285 - 1650309..1651583 (-) 1275 WP_161072600.1 HlyC/CorC family transporter -
  KT882_RS07290 - 1651753..1652985 (+) 1233 WP_228895112.1 right-handed parallel beta-helix repeat-containing protein -
  KT882_RS07295 - 1653002..1654339 (-) 1338 WP_228895113.1 sodium/glutamate symporter -
  KT882_RS07300 - 1654346..1654681 (-) 336 WP_228895115.1 PsiF family protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62352.92 Da        Isoelectric Point: 5.2843

>NTDB_id=624561 KT882_RS07280 WP_161072601.1 1648453..1650183(-) (pilF) [Pseudoduganella aquatica strain HC52]
MAAVQPNTASSAPMAGLARALMQAGRLTPAQADALQKKAQADKLPFIDTLLASNTIQARDLAYFCSETFAYPLMDLAAFS
IDALPPKIIDAKLMQTQRVVALAKRGNKMSVAISDPTNIQALDQIKFQTESSVEPVIVAHDALLAMLASLNRSAEQSLTE
LAGDEQDIQFAEENEAPSAAAEQAANEVEDAPIVRFLNKMLMDAVGMGASDLHFEPFEKFYRIRFRVDGVLLEHAQPPVS
IKDKLVSRIKVLAKLDISEKRVPQDGRMRLIVSPTKTIDLRVSTLPTLFGEKTVMRILDATQAQMGIDSLGYDPDQKEIL
LDAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDRAGLTFPVALKSFLRQDPDI
IMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVVLITAQRLARRLCTCKQPVEISNEL
LLKAGYKPEELDGSWKPYGPVGCERCNGGGYKGRVGIYQIMPITPAIESIILAHGNSMQIAAQSESEGVKSLRQSGLVKV
KNGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=624561 KT882_RS07280 WP_161072601.1 1648453..1650183(-) (pilF) [Pseudoduganella aquatica strain HC52]
ATGGCAGCAGTTCAACCCAATACGGCGTCCAGCGCCCCCATGGCGGGACTGGCGCGGGCCTTGATGCAAGCCGGCCGGCT
CACGCCCGCGCAGGCGGATGCGCTGCAAAAGAAGGCGCAGGCCGACAAGCTGCCGTTTATCGACACGCTGCTGGCCAGCA
ACACCATCCAGGCCCGCGATCTGGCCTACTTCTGTTCGGAAACGTTCGCCTATCCGCTGATGGACCTGGCCGCCTTCAGC
ATCGACGCGCTGCCGCCCAAGATCATCGACGCCAAGCTGATGCAGACGCAGCGCGTGGTGGCGCTGGCCAAGCGCGGCAA
CAAGATGTCGGTGGCCATTTCGGACCCGACCAATATCCAGGCGCTGGACCAGATCAAGTTCCAGACCGAGTCCTCGGTGG
AACCGGTGATCGTGGCGCACGACGCCCTGCTGGCCATGCTGGCCAGCCTGAACCGCAGCGCCGAACAAAGCCTGACCGAG
CTGGCCGGCGACGAGCAGGACATCCAGTTCGCCGAGGAAAACGAAGCGCCGTCGGCCGCCGCCGAGCAGGCCGCCAACGA
GGTGGAAGACGCGCCCATCGTGCGCTTCCTCAACAAGATGCTGATGGACGCGGTGGGCATGGGCGCCTCGGACTTGCATT
TCGAACCCTTCGAGAAGTTCTACCGCATCCGCTTCCGCGTGGACGGCGTGCTGCTGGAGCACGCGCAGCCGCCGGTGTCG
ATCAAGGACAAGCTGGTGTCGCGCATCAAGGTGCTGGCCAAGCTGGATATCTCGGAAAAGCGCGTGCCGCAGGACGGCCG
CATGCGTCTGATCGTCTCGCCCACCAAGACCATCGATTTGCGCGTGTCCACGCTGCCCACGCTGTTCGGCGAAAAAACCG
TGATGCGTATCCTGGACGCGACCCAGGCCCAGATGGGCATCGACTCGCTGGGCTACGACCCGGACCAGAAGGAAATCCTG
CTGGACGCCATCCAGCGCCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CTGCCTGAACATCCTGAACAAGCCCGGCATCAACATCTCCACCGCCGAGGATCCGGCCGAGATCAACCTGCCGGGCGTGA
ACCAGGTGAACGTGAACGACCGCGCCGGGCTGACCTTCCCGGTGGCGCTGAAATCCTTCCTGCGCCAGGATCCGGACATC
ATCATGGTGGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCCATCAAGGCGGCGCAGACCGGCCACATGGTGTTCTC
CACGCTGCACACCAACGACGCGCCGTCCACGCTGACGCGGCTGATGAATATGGGCGTGGCGCCGTTCAACATCGCCTCCT
CGGTGGTGCTGATCACGGCGCAGCGCCTGGCGCGCCGCCTGTGCACCTGCAAGCAGCCGGTGGAGATCTCCAACGAGCTG
CTGCTGAAAGCGGGCTACAAGCCGGAGGAGCTGGACGGCAGCTGGAAGCCCTACGGCCCGGTGGGCTGCGAGCGCTGCAA
CGGCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGATCATGCCGATCACCCCGGCCATCGAGAGCATCATCCTGGCGC
ACGGCAACTCGATGCAGATTGCGGCCCAGTCGGAAAGCGAAGGCGTGAAGTCCTTGCGCCAGTCCGGCCTGGTCAAAGTG
AAAAACGGGCTGACCAGCCTCGAGGAAGTCCTCGGCTGCACCAATGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X4HBN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.9

98.785

0.523
  pilB Acinetobacter baumannii D1279779

52.456

98.958

0.519
  pilB Acinetobacter baylyi ADP1

52.389

98.09

0.514
  pilB Legionella pneumophila strain ERS1305867

47.594

97.396

0.464
  pilB Vibrio cholerae strain A1552

45.359

99.132

0.45
  pilB Vibrio campbellii strain DS40M4

44.504

97.917

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

44.484

97.569

0.434
  pilF Thermus thermophilus HB27

38.272

98.438

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.79

97.396

0.368