Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M5T31_RS18470 Genome accession   NZ_CP103694
Coordinates   3757368..3758753 (+) Length   461 a.a.
NCBI ID   WP_032162574.1    Uniprot ID   -
Organism   Escherichia coli strain ST12468     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3752368..3763753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T31_RS18445 (M5T31_18445) aroP 3752916..3754289 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M5T31_RS18450 (M5T31_18450) ampE 3754332..3755186 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  M5T31_RS18455 (M5T31_18455) ampD 3755183..3755734 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5T31_RS18460 (M5T31_18460) nadC 3755822..3756715 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5T31_RS18465 (M5T31_18465) pilA 3756918..3757358 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  M5T31_RS18470 (M5T31_18470) pilB 3757368..3758753 (+) 1386 WP_032162574.1 type II secretion system protein GspE Machinery gene
  M5T31_RS18475 (M5T31_18475) hofC 3758743..3759945 (+) 1203 WP_000157266.1 protein transport protein HofC -
  M5T31_RS18480 (M5T31_18480) guaC 3759980..3761023 (-) 1044 WP_001217338.1 GMP reductase -
  M5T31_RS18485 (M5T31_18485) - 3761179..3761223 (-) 45 WP_120795372.1 protein YacM -
  M5T31_RS18490 (M5T31_18490) coaE 3761248..3761868 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  M5T31_RS18495 (M5T31_18495) zapD 3761868..3762611 (+) 744 WP_001194734.1 cell division protein ZapD -
  M5T31_RS18500 (M5T31_18500) yacG 3762621..3762818 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  M5T31_RS18505 (M5T31_18505) mutT 3763034..3763423 (-) 390 WP_000736007.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50596.28 Da        Isoelectric Point: 6.6143

>NTDB_id=624318 M5T31_RS18470 WP_032162574.1 3757368..3758753(+) (pilB) [Escherichia coli strain ST12468]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLHIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=624318 M5T31_RS18470 WP_032162574.1 3757368..3758753(+) (pilB) [Escherichia coli strain ST12468]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCATATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.236

100

0.443
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.476

82.863

0.41
  pilB Acinetobacter baylyi ADP1

39.744

100

0.403
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

45.897

84.599

0.388
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.401

85.466

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369