Detailed information NTG0622355

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: LL047_RS11540
• Genome accession: NZ_CP086072
• Coordinates: 2246536..2247609 (-)
• Length: 357 a.a.
• NCBI ID: WP_010906319.1
• Uniprot ID: A0A552YT77
• Organism: Lactococcus lactis subsp. lactis strain EIP33
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2241536..2252609

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL047_RS11495 (LL047_11460)	-	2241675..2242484 (-)	810	WP_010906310.1	metal ABC transporter permease	-
LL047_RS11500 (LL047_11465)	-	2242477..2243214 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
LL047_RS11505 (LL047_11470)	-	2243391..2244233 (-)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
LL047_RS11510 (LL047_11475)	-	2244230..2244667 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LL047_RS11515 (LL047_11480)	comGG	2244748..2245032 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
LL047_RS11520 (LL047_11485)	comGF	2245071..2245517 (-)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
LL047_RS11525 (LL047_11490)	comGE	2245480..2245776 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LL047_RS11530 (LL047_11495)	comGD	2245748..2246146 (-)	399	WP_021214886.1	competence type IV pilus minor pilin ComGD	Machinery gene
LL047_RS11535 (LL047_11500)	comGC	2246139..2246522 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
LL047_RS11540 (LL047_11505)	comGB	2246536..2247609 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL047_RS11545 (LL047_11510)	comGA	2247503..2248441 (-)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40330.51 Da
• Isoelectric Point: 10.3000

>NTDB_id=622355 LL047_RS11540 WP_010906319.1 2246536..2247609(-) (comGB) [Lactococcus lactis subsp. lactis strain EIP33]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=622355 LL047_RS11540 WP_010906319.1 2246536..2247609(-) (comGB) [Lactococcus lactis subsp. lactis strain EIP33]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

3D structure

• AlphaFold DB: A0A552YT77

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	83.578	95.518	0.798
comYB	Streptococcus gordonii str. Challis substr. CH1	53.959	95.518	0.515
comYB	Streptococcus mutans UA140	53.372	95.518	0.51
comYB	Streptococcus mutans UA159	53.079	95.518	0.507
comGB/cglB	Streptococcus mitis SK321	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae TIGR4	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae Rx1	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae D39	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae R6	51.045	93.838	0.479
comGB/cglB	Streptococcus mitis NCTC 12261	50.448	93.838	0.473