Detailed information NTG0061912

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SIR_RS18210
• Genome accession: NC_022246
• Coordinates: 1758855..1759292 (-)
• Length: 145 a.a.
• NCBI ID: WP_003073990.1
• Uniprot ID: -
• Organism: Streptococcus intermedius B196
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1753855..1764292

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SIR_RS18190 (SIR_1660)	glmS	1754054..1755868 (-)	1815	WP_021003253.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
SIR_RS18195 (SIR_1661)	-	1756258..1757457 (-)	1200	WP_021003254.1	acetate kinase	-
SIR_RS18200 (SIR_1662)	comYH	1757504..1758457 (-)	954	WP_021003255.1	class I SAM-dependent methyltransferase	Machinery gene
SIR_RS18205 (SIR_1663)	comGG	1758548..1758874 (-)	327	WP_021003256.1	competence type IV pilus minor pilin ComGG	-
SIR_RS18210 (SIR_1664)	comGF/cglF	1758855..1759292 (-)	438	WP_003073990.1	competence type IV pilus minor pilin ComGF	Machinery gene
SIR_RS18215 (SIR_1665)	comGE/cglE	1759276..1759569 (-)	294	WP_003073987.1	competence type IV pilus minor pilin ComGE	Machinery gene
SIR_RS18220 (SIR_1666)	comYD	1759541..1759969 (-)	429	WP_021003257.1	competence type IV pilus minor pilin ComGD	Machinery gene
SIR_RS18225 (SIR_1667)	comYC	1759929..1760246 (-)	318	WP_003075998.1	competence type IV pilus major pilin ComGC	Machinery gene
SIR_RS18230 (SIR_1668)	comYB	1760243..1761265 (-)	1023	WP_021003258.1	competence type IV pilus assembly protein ComGB	Machinery gene
SIR_RS18235 (SIR_1669)	comYA	1761207..1762148 (-)	942	WP_003073980.1	competence type IV pilus ATPase ComGA	Machinery gene
SIR_RS18240 (SIR_1670)	-	1762217..1762582 (-)	366	WP_021003259.1	DUF1033 family protein	-
SIR_RS18245 (SIR_1671)	glnA	1762804..1764150 (-)	1347	WP_021003260.1	type I glutamate--ammonia ligase	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16858.51 Da
• Isoelectric Point: 10.2149

>NTDB_id=61912 SIR_RS18210 WP_003073990.1 1758855..1759292(-) (comGF/cglF) [Streptococcus intermedius B196]
MFKANKVKAFTLLEALIALFVLSGSVLLFQSMTRLLASEVQTRQHSEQREWLLFADQLEVELTRSSFEKVENNRLYMKQD
GKVIAFGKASGQDFRKTNANGKGYQPMVYDVKKAEISQENELVRIRLLFKRGLEREFIYRVETKS

Nucleotide

• Length: 438 bp

>NTDB_id=61912 SIR_RS18210 WP_003073990.1 1758855..1759292(-) (comGF/cglF) [Streptococcus intermedius B196]
GTGTTCAAAGCAAATAAAGTAAAAGCTTTTACACTTTTAGAGGCTCTGATTGCCCTATTCGTACTCAGTGGAAGTGTGTT
GCTATTTCAATCTATGACGCGTCTTCTGGCTTCTGAAGTCCAAACTCGACAACACAGTGAACAGCGAGAATGGCTTTTGT
TTGCCGATCAACTAGAAGTGGAGTTGACGCGTTCTTCATTTGAAAAAGTGGAGAATAATCGTTTATACATGAAGCAAGAT
GGTAAGGTAATTGCATTTGGAAAAGCAAGTGGACAAGATTTCAGGAAAACCAATGCGAATGGGAAAGGTTATCAGCCCAT
GGTTTATGATGTGAAAAAAGCAGAAATTTCACAGGAAAATGAGCTAGTTCGTATTCGATTACTGTTTAAGAGAGGATTGG
AAAGGGAATTCATCTATCGTGTGGAAACAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	68.571	96.552	0.662
comGF/cglF	Streptococcus pneumoniae TIGR4	68.571	96.552	0.662
comGF/cglF	Streptococcus pneumoniae Rx1	68.571	96.552	0.662
comGF/cglF	Streptococcus pneumoniae D39	68.571	96.552	0.662
comGF/cglF	Streptococcus mitis NCTC 12261	67.857	96.552	0.655
comGF/cglF	Streptococcus mitis SK321	67.857	96.552	0.655
comYF	Streptococcus mutans UA159	41.135	97.241	0.4
comYF	Streptococcus mutans UA140	41.135	97.241	0.4
comGF	Lactococcus lactis subsp. cremoris KW2	42.963	93.103	0.4