Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   NW342_RS25795 Genome accession   NZ_CP102944
Coordinates   5500977..5501516 (-) Length   179 a.a.
NCBI ID   WP_258383150.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SCAID TCT1-2022 (325)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5495977..5506516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NW342_RS25775 (NW342_25775) - 5496662..5498917 (-) 2256 WP_033990664.1 DUF1631 domain-containing protein -
  NW342_RS25780 (NW342_25780) nadC 5499203..5500051 (+) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NW342_RS25790 (NW342_25790) - 5500215..5500913 (-) 699 WP_223677410.1 hypothetical protein -
  NW342_RS25795 (NW342_25795) pilE 5500977..5501516 (-) 540 WP_258383150.1 pilin Machinery gene
  NW342_RS25800 (NW342_25800) pilB 5501745..5503448 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NW342_RS25805 (NW342_25805) pilC 5503452..5504669 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  NW342_RS25810 (NW342_25810) pilD 5504672..5505544 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  NW342_RS25815 (NW342_25815) coaE 5505541..5506152 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  NW342_RS25820 (NW342_25820) yacG 5506149..5506349 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18071.48 Da        Isoelectric Point: 6.2547

>NTDB_id=618649 NW342_RS25795 WP_258383150.1 5500977..5501516(-) (pilE) [Pseudomonas aeruginosa strain SCAID TCT1-2022 (325)]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATSVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASQSNAVSSGLDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=618649 NW342_RS25795 WP_258383150.1 5500977..5501516(-) (pilE) [Pseudomonas aeruginosa strain SCAID TCT1-2022 (325)]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAGCGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCAATCCAATGCGGTTTCCAGTGGTCTCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.956

100

0.447
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.889

100

0.391
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363