Detailed information NTG0618627

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HQN37_RS12175
• Genome accession: NZ_CP085505
• Coordinates: 2506301..2506915 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain BT2.4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2501301..2511915

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HQN37_RS12145 (HQN37_012145)	rpsT	2501604..2501870 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HQN37_RS12150 (HQN37_012150)	holA	2501887..2502928 (-)	1042	Protein_2345	DNA polymerase III subunit delta	-
HQN37_RS12155 (HQN37_012155)	-	2502968..2503119 (-)	152	Protein_2346	hypothetical protein	-
HQN37_RS12160 (HQN37_012160)	-	2503160..2503294 (+)	135	WP_003152870.1	YqzM family protein	-
HQN37_RS12165 (HQN37_012165)	comEC	2503313..2505664 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HQN37_RS12170 (HQN37_012170)	-	2505665..2506234 (-)	570	WP_003152868.1	ComE operon protein 2	-
HQN37_RS12175 (HQN37_012175)	comEA	2506301..2506915 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
HQN37_RS12180 (HQN37_012180)	comER	2506974..2507795 (+)	822	WP_012118027.1	late competence protein ComER	-
HQN37_RS12185 (HQN37_012185)	-	2507864..2508601 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
HQN37_RS12190 (HQN37_012190)	rsfS	2508598..2508954 (-)	357	WP_007408260.1	ribosome silencing factor	-
HQN37_RS12195 (HQN37_012195)	yqeK	2508971..2509531 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HQN37_RS12200 (HQN37_012200)	-	2509521..2510090 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
HQN37_RS12205 (HQN37_012205)	yhbY	2510101..2510391 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
HQN37_RS12210 (HQN37_012210)	aroE	2510385..2511227 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=618627 HQN37_RS12175 WP_012118026.1 2506301..2506915(-) (comEA) [Bacillus velezensis strain BT2.4]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=618627 HQN37_RS12175 WP_012118026.1 2506301..2506915(-) (comEA) [Bacillus velezensis strain BT2.4]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363