Detailed information NTG0618553

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HQN47_RS12180
• Genome accession: NZ_CP085504
• Coordinates: 2506685..2507299 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain BP1.2A
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2501685..2512299

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HQN47_RS12150 (HQN47_012150)	rpsT	2501988..2502254 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HQN47_RS12155 (HQN47_012155)	holA	2502271..2503312 (-)	1042	Protein_2346	DNA polymerase III subunit delta	-
HQN47_RS12160 (HQN47_012160)	-	2503352..2503503 (-)	152	Protein_2347	hypothetical protein	-
HQN47_RS12165 (HQN47_012165)	-	2503544..2503678 (+)	135	WP_003152870.1	YqzM family protein	-
HQN47_RS12170 (HQN47_012170)	comEC	2503697..2506048 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HQN47_RS12175 (HQN47_012175)	-	2506049..2506618 (-)	570	WP_003152868.1	ComE operon protein 2	-
HQN47_RS12180 (HQN47_012180)	comEA	2506685..2507299 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
HQN47_RS12185 (HQN47_012185)	comER	2507358..2508179 (+)	822	WP_012118027.1	late competence protein ComER	-
HQN47_RS12190 (HQN47_012190)	-	2508248..2508985 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
HQN47_RS12195 (HQN47_012195)	rsfS	2508982..2509338 (-)	357	WP_007408260.1	ribosome silencing factor	-
HQN47_RS12200 (HQN47_012200)	yqeK	2509355..2509915 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HQN47_RS12205 (HQN47_012205)	-	2509905..2510474 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
HQN47_RS12210 (HQN47_012210)	yhbY	2510485..2510775 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
HQN47_RS12215 (HQN47_012215)	aroE	2510769..2511611 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=618553 HQN47_RS12180 WP_012118026.1 2506685..2507299(-) (comEA) [Bacillus velezensis strain BP1.2A]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=618553 HQN47_RS12180 WP_012118026.1 2506685..2507299(-) (comEA) [Bacillus velezensis strain BP1.2A]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363