Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LIS79_RS16990 Genome accession   NZ_CP085389
Coordinates   3247660..3248787 (-) Length   375 a.a.
NCBI ID   WP_074041808.1    Uniprot ID   -
Organism   Bacillus altitudinis strain CMF5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3242660..3253787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS79_RS16955 (LIS79_16965) flgL 3242843..3243754 (-) 912 WP_017368141.1 flagellar hook-associated protein FlgL -
  LIS79_RS16960 (LIS79_16970) flgK 3243763..3245283 (-) 1521 WP_017368142.1 flagellar hook-associated protein FlgK -
  LIS79_RS16965 (LIS79_16975) - 3245299..3245781 (-) 483 WP_017357865.1 flagellar protein FlgN -
  LIS79_RS16970 (LIS79_16980) flgM 3245800..3246063 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  LIS79_RS16975 (LIS79_16985) - 3246143..3246562 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  LIS79_RS16980 (LIS79_16990) comFC 3246619..3247305 (-) 687 WP_169476072.1 ComF family protein Machinery gene
  LIS79_RS16985 (LIS79_16995) - 3247302..3247595 (-) 294 WP_035701582.1 competence protein ComFB -
  LIS79_RS16990 (LIS79_17000) comFA 3247660..3248787 (-) 1128 WP_074041808.1 DEAD/DEAH box helicase Machinery gene
  LIS79_RS16995 (LIS79_17005) - 3249159..3250001 (-) 843 WP_024719242.1 DegV family protein -
  LIS79_RS17000 (LIS79_17010) degU 3250213..3250902 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  LIS79_RS17005 (LIS79_17015) degS 3250967..3252142 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  LIS79_RS17010 (LIS79_17020) - 3252364..3253032 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42949.96 Da        Isoelectric Point: 10.4871

>NTDB_id=617370 LIS79_RS16990 WP_074041808.1 3247660..3248787(-) (comFA) [Bacillus altitudinis strain CMF5]
MLGKVSECGFLYEWTGPQKKETRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVIELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKRLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQVQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
TESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNSAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=617370 LIS79_RS16990 WP_074041808.1 3247660..3248787(-) (comFA) [Bacillus altitudinis strain CMF5]
ATGCTGGGAAAAGTAAGTGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCACGGAATAAAATATGCGTTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTAATTGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCACCGCTTATGATCGCTACAACTCATCAGCTAATGA
GATATAAAAACGCTTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAATCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAGATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGTTCAAGATGTGAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGGTACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAAGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
ACAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATTCAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.405

98.667

0.547
  comFA/cflA Streptococcus pneumoniae Rx1

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae D39

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae R6

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae TIGR4

40.678

94.4

0.384
  comFA/cflA Streptococcus mitis NCTC 12261

40.395

94.4

0.381
  comFA Latilactobacillus sakei subsp. sakei 23K

37.903

99.2

0.376
  comFA/cflA Streptococcus mitis SK321

39.266

94.4

0.371
  comFA Lactococcus lactis subsp. cremoris KW2

37.705

97.6

0.368