Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LIN44_RS03605 Genome accession   NZ_CP085344
Coordinates   763001..764725 (-) Length   574 a.a.
NCBI ID   WP_227313539.1    Uniprot ID   -
Organism   Cupriavidus sp. MP-37     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 758001..769725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN44_RS03580 (LIN44_03580) - 758879..759067 (-) 189 WP_018006248.1 DNA gyrase inhibitor YacG -
  LIN44_RS03585 (LIN44_03585) zapD 759085..759843 (-) 759 WP_012353939.1 cell division protein ZapD -
  LIN44_RS03590 (LIN44_03590) coaE 760052..760678 (-) 627 WP_227313536.1 dephospho-CoA kinase -
  LIN44_RS03595 (LIN44_03595) pilD 760761..761660 (-) 900 WP_227313537.1 A24 family peptidase Machinery gene
  LIN44_RS03600 (LIN44_03600) - 761664..762935 (-) 1272 WP_227313538.1 type II secretion system F family protein -
  LIN44_RS03605 (LIN44_03605) pilB 763001..764725 (-) 1725 WP_227313539.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LIN44_RS03610 (LIN44_03610) - 765047..765385 (+) 339 WP_227313540.1 type II toxin-antitoxin system RelE/ParE family toxin -
  LIN44_RS03615 (LIN44_03615) - 765375..765686 (+) 312 WP_227313541.1 helix-turn-helix domain-containing protein -
  LIN44_RS03625 (LIN44_03625) ispB 765942..766871 (-) 930 WP_227314332.1 octaprenyl diphosphate synthase -
  LIN44_RS03630 (LIN44_03630) rplU 767351..767662 (+) 312 WP_006576525.1 50S ribosomal protein L21 -
  LIN44_RS03635 (LIN44_03635) rpmA 767702..767962 (+) 261 WP_092306246.1 50S ribosomal protein L27 -
  LIN44_RS03640 (LIN44_03640) obgE 768093..769190 (+) 1098 WP_227313542.1 GTPase ObgE -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63274.91 Da        Isoelectric Point: 7.0569

>NTDB_id=617224 LIN44_RS03605 WP_227313539.1 763001..764725(-) (pilB) [Cupriavidus sp. MP-37]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLAQYNLSKVPPALAGNREF
HAHRLLPLGRRDNRLVLALSDPSNQAGLDAIRDKYKLPVEAVVVEHDKLMKHVRAAGEALGTLKNISPVQAERKMIEYDP
VAAAANPRNRTTADNIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDKDAKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAAFNIASSVLMITAQRLARRLCTCKREGEIPREALL
EAGFREQDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQEIILAHGTALQIAEQARKDGVLSLREAGLLKVRQ
GVTSLEEVLATTNT

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=617224 LIN44_RS03605 WP_227313539.1 763001..764725(-) (pilB) [Cupriavidus sp. MP-37]
ATGACCCTCGGACTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCACGTGA
AAAGCAATCGCAGCTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCGCTGTTCGCCGCCGACA
AGTACCAGCTGCCGCTGCTGGACCTGGCCCAGTACAACCTCTCCAAGGTGCCGCCGGCGCTGGCTGGCAACCGTGAATTC
CATGCCCACCGGCTGCTGCCGCTGGGCCGGCGCGACAACCGCCTGGTGCTGGCGCTGTCCGATCCGTCCAACCAGGCCGG
CCTGGATGCGATCCGCGACAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCATGTGC
GCGCCGCCGGCGAGGCCCTGGGGACGCTGAAGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGATCCG
GTCGCCGCCGCCGCCAACCCGCGCAACCGCACCACGGCCGACAACATCGACGACGCCCCGGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAGGCCTTCCACCGCGGCGCGTCGGACCTGCACTTCGAACCGTTCGAAACTTTCTACCGCATCCGCTTCC
GCGTCGATGGCGTGCTGCAGGAAGTCGCGCGCCCGCCGCTGGATATCCGCGACAAGATCGCGACCCGGATCAAGGTGCTG
TCGCGGCTGGATATTTCTGAAAAGCGTGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGACAAGGA
TGCCAAGGAAACCGTCGAGAAGGCGGTGGACTTCCGCGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGC
GGATCCTGGAATCGTCGTCCGACAAGCTCGACATCGACCAGCTCGGCTACGAGCCGGAGCAGAAGGCGCTGCTGCTGGAC
GTGATCAAGCGCCCGTACGGCATGGTGCTGGTGACCGGCCCCACGGGCAGCGGCAAGACCGTGTCGCTGTACACCTTCCT
GAACCTGCTGAACCAGGGCGACATCAATATCTCCACGGCCGAGGACCCAGCCGAAATCCAGCTGCCGGGCATCAACCAGG
TCAACGTCAACGACAAGGCCGGGCTGACCTTTGCCGCCGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGACATTTCCATCAAGGCCGCGCAGACCGGCCACCTGGTGTTGTCGACGCT
GCACACCAACGACGCGCCGACCACGCTGACCCGCCTGATGAACATGGGCGTGGCCGCCTTCAACATTGCCTCGAGCGTGC
TGATGATCACCGCGCAGCGGCTGGCGCGGCGGCTGTGCACCTGCAAGCGCGAGGGCGAGATCCCGCGCGAGGCCTTGCTG
GAAGCGGGCTTCCGCGAGCAGGACCTGGACGGCAGCTGGCAGCCCTACCATCCGGTCGGCTGCGAGCGCTGCAACGGCAG
CGGCTACAAGGGCCGCTGCGGCATCTACCAGGTGATGCCGATCACCGAGGCCATGCAGGAGATCATCCTGGCGCACGGCA
CCGCGCTGCAGATTGCCGAGCAGGCGCGCAAGGACGGCGTGCTATCGTTGCGCGAAGCGGGGCTGCTGAAGGTCAGGCAG
GGCGTCACGTCACTCGAAGAAGTGCTGGCGACCACAAATACGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(187-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.265

100

0.523
  pilF Neisseria gonorrhoeae MS11

51.813

100

0.523
  pilB Acinetobacter baylyi ADP1

52.269

95.993

0.502
  pilB Legionella pneumophila strain ERS1305867

50.091

95.993

0.481
  pilB Vibrio cholerae strain A1552

45.694

99.129

0.453
  pilB Vibrio campbellii strain DS40M4

45.841

94.251

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

45.756

94.425

0.432
  pilF Thermus thermophilus HB27

38.687

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.989

96.516

0.376