Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LG323_RS05435 Genome accession   NZ_CP085242
Coordinates   1141457..1143172 (+) Length   571 a.a.
NCBI ID   WP_404419729.1    Uniprot ID   -
Organism   Marinospirillum sp. strain ABH-597     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1136457..1148172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG323_RS05395 (LG323_05405) - 1136468..1137127 (+) 660 WP_404419714.1 Bax inhibitor-1/YccA family protein -
  LG323_RS05400 (LG323_05410) tusD 1137131..1137526 (+) 396 WP_404419716.1 sulfurtransferase complex subunit TusD -
  LG323_RS05405 (LG323_05415) tusC 1137523..1137891 (+) 369 WP_404419718.1 sulfurtransferase complex subunit TusC -
  LG323_RS05410 (LG323_05420) tusB 1137893..1138171 (+) 279 WP_404419719.1 sulfurtransferase complex subunit TusB -
  LG323_RS05415 (LG323_05425) - 1138155..1138487 (+) 333 WP_404419721.1 TusE/DsrC/DsvC family sulfur relay protein -
  LG323_RS05420 (LG323_05430) - 1138553..1139647 (+) 1095 WP_404419723.1 3-deoxy-7-phosphoheptulonate synthase -
  LG323_RS05425 (LG323_05435) - 1139737..1140423 (-) 687 WP_404419725.1 DUF5610 domain-containing protein -
  LG323_RS05430 (LG323_05440) - 1140695..1141090 (-) 396 WP_404419727.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LG323_RS05435 (LG323_05450) pilB 1141457..1143172 (+) 1716 WP_404419729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LG323_RS05440 (LG323_05455) pilC 1143184..1144404 (+) 1221 WP_404419731.1 type II secretion system F family protein Machinery gene
  LG323_RS05445 (LG323_05460) - 1144404..1145276 (+) 873 WP_404419733.1 prepilin peptidase -
  LG323_RS05450 (LG323_05465) - 1145266..1146588 (-) 1323 WP_404419734.1 replication-associated recombination protein A -
  LG323_RS05455 (LG323_05470) lolA 1146590..1147210 (-) 621 WP_404419736.1 outer membrane lipoprotein chaperone LolA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62621.77 Da        Isoelectric Point: 5.3237

>NTDB_id=616299 LG323_RS05435 WP_404419729.1 1141457..1143172(+) (pilB) [Marinospirillum sp. strain ABH-597]
MVDVSNPHVLSSLSKRLIRDGLLDEGEALAAQVKAKENGLSFSHYLNSTGHVDSDQLANLIAEEFGLPYLDLEAIDPKQF
PKKLVEENLLRKHQALPILLRDNQLSLALSTPTNLAAIDEIQFQTGLIINPIIVSETKLHQILEASLETDIGSLGDLDDS
ELEGLEAGSTQVEEELSGDAVGGKDDAPIVKFVNKVLLDAIRRGASDIHFEPYEHSYRVRCRIDGILHEMTRPPLSLRGR
LAARLKVMAKLNISERRIPQDGGIKLRLSKTRAIDFRVNTLPTLWGEKIVLRILDANAAKMGIEQLGFEAEQQKLYEQAL
ANPQGMILVTGPTGSGKTVTLYTGLNLLNSPERNIASAEDPVEINMDGINQVNINTKVGLDFASALRAFLRQDPDVVMVG
EIRDIETAETAVKAAQTGHLVLSTLHTNSAAATLNRLMNMGVPAYNLAASVQLIIAQRLARRLCPKCKKPSELPQDTLLE
LGFSEQELAENQIYDAVGCSQCTKGYKGRLGIYEVVKITPELADLVMHSASAMEISRKAREQGFPDLRQSGRRKVLAGLT
SVDELTRVTME

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=616299 LG323_RS05435 WP_404419729.1 1141457..1143172(+) (pilB) [Marinospirillum sp. strain ABH-597]
ATGGTGGATGTTTCCAATCCTCATGTTCTCAGCTCTCTCTCCAAGCGCCTGATTCGCGATGGTTTGCTGGATGAAGGAGA
AGCTCTCGCTGCGCAGGTCAAAGCCAAGGAGAATGGTCTGTCTTTCAGCCACTACCTGAACAGCACAGGTCATGTAGACA
GCGATCAACTGGCCAATCTGATTGCTGAGGAATTCGGGCTACCCTATCTGGATCTGGAAGCCATTGATCCCAAGCAATTC
CCCAAAAAGCTGGTGGAAGAAAACCTGCTGCGCAAGCATCAGGCTCTACCGATTCTATTGCGTGATAACCAGTTGTCTCT
GGCTCTTTCCACGCCGACCAACCTGGCTGCCATTGATGAAATCCAATTCCAGACCGGGCTGATCATTAACCCGATTATTG
TTTCCGAAACCAAGCTGCACCAAATTCTGGAAGCATCACTGGAAACCGACATCGGTTCTTTGGGGGATCTGGATGACTCC
GAATTGGAAGGTTTGGAAGCTGGCAGCACCCAGGTTGAAGAAGAGCTGAGCGGCGATGCAGTGGGTGGCAAGGATGATGC
ACCTATTGTAAAGTTTGTAAACAAAGTGTTGCTAGATGCCATACGCCGGGGCGCTTCGGACATTCATTTTGAACCCTATG
AGCACAGTTATCGGGTTCGCTGTCGTATTGATGGCATTCTGCATGAGATGACCCGCCCGCCCTTGTCACTGCGGGGCCGC
CTTGCTGCACGTTTGAAGGTCATGGCCAAGCTGAATATTTCCGAGAGGCGTATTCCCCAGGATGGTGGCATCAAACTAAG
GCTTTCCAAAACCCGGGCCATAGATTTTCGTGTGAATACCCTGCCGACCCTCTGGGGCGAAAAAATCGTTTTGCGGATAC
TGGATGCCAATGCCGCCAAAATGGGTATAGAGCAGTTGGGCTTTGAAGCCGAGCAACAAAAACTCTATGAGCAGGCCTTG
GCCAACCCTCAAGGCATGATTCTGGTCACCGGCCCCACGGGCAGTGGTAAAACGGTTACTCTTTATACCGGCCTGAATCT
GCTGAACTCGCCTGAGCGCAATATTGCCAGTGCTGAAGATCCGGTTGAAATCAATATGGATGGCATCAACCAGGTCAACA
TCAATACCAAGGTGGGGCTGGATTTTGCTTCGGCATTACGGGCTTTTTTGCGCCAGGATCCGGATGTGGTTATGGTGGGC
GAGATTCGTGATATAGAAACAGCAGAAACAGCCGTCAAGGCCGCCCAGACGGGGCACCTGGTGCTTTCCACCCTGCACAC
CAACTCAGCTGCAGCCACTCTGAACCGTTTGATGAACATGGGGGTTCCAGCCTACAACCTGGCCGCTTCTGTACAATTGA
TCATAGCCCAGCGCCTGGCCAGGCGGCTTTGTCCGAAATGCAAAAAACCTTCCGAACTGCCTCAGGATACGCTGCTGGAG
CTGGGCTTCAGCGAACAGGAACTGGCGGAAAACCAGATTTATGATGCAGTAGGCTGCAGCCAGTGCACCAAGGGTTACAA
GGGGCGTTTGGGGATTTATGAGGTGGTCAAAATCACCCCGGAACTGGCAGATCTGGTGATGCATTCCGCCAGTGCCATGG
AGATCAGTCGCAAGGCCCGTGAGCAGGGCTTTCCTGATTTGCGTCAGTCAGGCCGCAGAAAGGTTCTTGCCGGTCTGACC
AGTGTCGATGAACTAACCCGCGTCACCATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

52.696

100

0.531
  pilB Acinetobacter baumannii D1279779

55.009

96.147

0.529
  pilB Legionella pneumophila strain ERS1305867

52.174

100

0.525
  pilB Vibrio cholerae strain A1552

50

99.124

0.496
  pilB Vibrio campbellii strain DS40M4

49.387

100

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

49.385

99.65

0.492
  pilF Neisseria gonorrhoeae MS11

47.458

92.995

0.441
  pilB/pilB1 Synechocystis sp. PCC 6803

35.588

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.387

96.497

0.361