Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LG312_RS00015 Genome accession   NZ_CP085221
Coordinates   2511..4307 (-) Length   598 a.a.
NCBI ID   WP_404297355.1    Uniprot ID   -
Organism   Halomonas sp. strain DBC-59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..9307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG312_RS00005 (LG312_00005) - 12..1130 (+) 1119 WP_404297303.1 ISAs1 family transposase -
  LG312_RS00010 (LG312_00010) pilC 1232..2467 (-) 1236 WP_404297353.1 type II secretion system F family protein Machinery gene
  LG312_RS00015 (LG312_00015) pilB 2511..4307 (-) 1797 WP_404297355.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LG312_RS00020 (LG312_00020) pilA 4649..5092 (+) 444 WP_404299546.1 pilin Machinery gene
  LG312_RS00025 (LG312_00025) - 5205..6932 (-) 1728 WP_404297357.1 Wzy polymerase domain-containing protein -
  LG312_RS00030 (LG312_00030) - 7170..7592 (+) 423 WP_379761379.1 phasin family protein -
  LG312_RS00035 (LG312_00035) - 7734..8255 (+) 522 WP_404297359.1 hypothetical protein -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 65324.01 Da        Isoelectric Point: 6.2964

>NTDB_id=615975 LG312_RS00015 WP_404297355.1 2511..4307(-) (pilB) [Halomonas sp. strain DBC-59]
MNHYHTSPPPPGAPHDSVLESTRNIDTTGLRGLARRLVVDGRINHAAALRAEEEAREGEVSLMEHVIDTGLVSARQATLS
AAWEYGLPMLDLDAIRPETLPKSDAFPEKILRKLKVLPLMQHGHRLTVAVPYPSTLALLDELQFATGLTPEGVLAPVDQL
GPVLESYLASSEKSMLDELKGVNDAIGELGFSGDDEAGGEVDIQAAANAASEDAPIIKFVNKILLDAIHRGASDIHFEPY
ETSYRVRFRIDGMLHDVAHPPFAMRTRIAARLKVMSRLDISERRLPQDGAIKLKLSKTRSIDFRVNSLPTVFGEKIVLRI
LDPASAQLGIEQLGFTPEQRKMYEIVLDQPQGMILVTGPTGSGKTVTLYTGINLLNEIERNICTAEDPVEIKVPGVNQVN
VLPKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGLAPFNIASSVSL
IIAQRLARKLCKHCKQPADLPHEALAAQGFSEEEINQATFFEPVGCKHCTKGFKGRVGIYEVVPISHAMSQLIMKNGNSM
DFHELARQEGHPDLRRSGLLKAMQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=615975 LG312_RS00015 WP_404297355.1 2511..4307(-) (pilB) [Halomonas sp. strain DBC-59]
ATGAACCACTACCACACTTCTCCGCCCCCGCCAGGCGCTCCTCATGATTCCGTTCTGGAGAGCACCCGCAACATCGACAC
CACCGGGCTGCGCGGCTTGGCGCGTCGCCTGGTGGTGGACGGGCGCATCAATCATGCCGCTGCGCTGCGTGCCGAAGAGG
AGGCGCGCGAGGGTGAAGTGTCGTTGATGGAGCACGTGATCGACACCGGGCTGGTCTCCGCTCGCCAGGCCACGCTCTCT
GCGGCCTGGGAGTATGGCCTGCCGATGCTGGACCTGGATGCCATCCGCCCGGAAACGCTGCCAAAGTCGGATGCCTTCCC
CGAAAAGATCCTGCGCAAGCTCAAGGTACTGCCGCTGATGCAGCACGGCCACCGGCTGACCGTGGCCGTGCCCTACCCCT
CCACCCTGGCGCTGCTCGATGAACTGCAGTTCGCGACCGGCTTGACCCCGGAAGGCGTGCTGGCCCCGGTGGATCAGCTC
GGCCCGGTGCTGGAGAGCTATCTCGCCTCCAGCGAGAAGAGCATGCTAGACGAGCTCAAGGGCGTGAATGACGCCATCGG
CGAACTGGGCTTCAGCGGCGATGACGAAGCCGGCGGCGAGGTGGATATTCAGGCGGCGGCCAACGCCGCCAGCGAAGACG
CACCGATCATCAAGTTCGTCAACAAGATCCTGCTGGACGCCATCCATCGCGGCGCCTCGGATATCCACTTCGAGCCCTAC
GAGACCAGCTACCGGGTGCGCTTTCGCATCGACGGCATGCTCCACGATGTAGCCCACCCGCCGTTTGCCATGCGCACGCG
CATCGCCGCGCGCCTCAAGGTCATGTCGCGACTTGATATCTCAGAGCGCCGCCTGCCCCAGGATGGCGCCATCAAGCTCA
AGCTCTCCAAGACCCGCTCCATCGATTTTCGCGTCAACTCGCTGCCCACGGTGTTCGGTGAGAAGATCGTGCTGCGTATT
CTCGACCCCGCCTCGGCTCAGCTGGGCATCGAGCAGCTCGGTTTTACGCCCGAGCAGCGCAAGATGTACGAAATCGTGCT
GGACCAGCCCCAGGGCATGATCCTGGTTACCGGCCCCACCGGCAGCGGCAAGACGGTGACCCTCTATACCGGCATCAACC
TGCTCAACGAGATCGAGCGCAACATCTGTACCGCCGAAGACCCGGTAGAGATCAAGGTGCCCGGGGTCAACCAGGTGAAC
GTGCTCCCCAAGATCGGCCTGGACTTCGCCAGCGCCCTGCGTGCCTTCCTGCGCCAGGACCCGGACGTGGTGATGGTGGG
CGAGATCCGCGACCTGGAAACCGCCGAGATCGCGGTCAAGGCCTCCCAGACCGGTCACCTGGTGCTCTCCACGGTGCACA
CCAACTCCGCCGCGGAAACCCTGACCCGCCTGGCCAACATGGGCCTGGCGCCCTTCAATATCGCCAGCTCCGTGAGCCTG
ATCATCGCCCAGCGCCTGGCGCGCAAGCTGTGCAAGCATTGCAAGCAGCCGGCGGATCTCCCCCACGAGGCGCTGGCCGC
CCAGGGGTTCTCCGAAGAGGAGATCAATCAGGCCACCTTCTTCGAGCCGGTGGGGTGCAAGCACTGCACCAAGGGTTTCA
AGGGCCGGGTGGGCATCTACGAGGTGGTGCCGATCAGCCACGCCATGAGCCAGCTGATCATGAAGAACGGCAACTCCATG
GATTTCCACGAGCTGGCCCGCCAGGAAGGCCACCCGGACCTGCGCCGCAGCGGCCTGCTGAAGGCCATGCAAGGGGTGAC
CAGCCTGGCCGAGATCAACCGCGTAACGAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(222-489)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.632

95.318

0.502
  pilB Acinetobacter baylyi ADP1

51.724

96.99

0.502
  pilB Acinetobacter baumannii D1279779

51.494

95.151

0.49
  pilB Vibrio campbellii strain DS40M4

49.177

91.472

0.45
  pilB Vibrio cholerae strain A1552

49.722

90.134

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

47.985

91.304

0.438
  pilF Neisseria gonorrhoeae MS11

45.009

95.485

0.43