Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LIN95_RS00515 Genome accession   NZ_CP085094
Coordinates   99757..101166 (+) Length   469 a.a.
NCBI ID   WP_005567970.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 5S     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 94757..106166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN95_RS00495 pnp 95235..97364 (-) 2130 WP_233119032.1 polyribonucleotide nucleotidyltransferase -
  LIN95_RS00500 nanQ 97658..98128 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  LIN95_RS00505 ampD 98602..99156 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LIN95_RS00510 pilA 99278..99730 (+) 453 WP_005586498.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LIN95_RS00515 pilB 99757..101166 (+) 1410 WP_005567970.1 GspE/PulE family protein Machinery gene
  LIN95_RS00520 pilC 101159..102382 (+) 1224 WP_005567968.1 type II secretion system F family protein Machinery gene
  LIN95_RS00525 - 102379..103068 (+) 690 WP_005547547.1 A24 family peptidase -
  LIN95_RS00530 coaE 103118..103741 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  LIN95_RS00535 yacG 103731..103943 (+) 213 WP_005567966.1 DNA gyrase inhibitor YacG -
  LIN95_RS00540 - 103943..104215 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -
  LIN95_RS00545 - 104612..105985 (+) 1374 WP_005547554.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52539.81 Da        Isoelectric Point: 6.2867

>NTDB_id=615469 LIN95_RS00515 WP_005567970.1 99757..101166(+) (pilB) [Aggregatibacter actinomycetemcomitans strain 5S]
MQNPMAQGYLIHSQNHGDFTIAPELWLKNQQQQTVLLRYLALPLAENGQTLWLGLDSLTNLSACEAFSFLTGKTIEPVLI
ESQTLKTALQDLSPRVEKVEENQPLFYSALSQEQPTQPYDEPIIRLLNDIFESATAQKASDIHLEPQADCLQTRFRIDGV
LQVQNTVPLSIASRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDTLDFRLSTLATQFGEKAVLRLQQNRPVQLAFGEL
GMTESQQQRFRRALSQPQGLILVTGPTGSGKSISLYTALQYLNTSEKHIMTAEDPIEIQLPGIIQSQVNHSIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQRHEIDGSLLLVIAQRLVRKRCQK
CGGNATRFCDCHQGYKGRVGVYQFLQPNLQHPQGYETDFADLGQSALEKLKDGTTDLTEIQRVLGQTHD

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=615469 LIN95_RS00515 WP_005567970.1 99757..101166(+) (pilB) [Aggregatibacter actinomycetemcomitans strain 5S]
ATGCAAAATCCGATGGCGCAAGGTTATTTGATTCACAGCCAAAATCACGGGGACTTTACCATTGCGCCCGAGCTATGGCT
TAAAAATCAGCAACAACAAACCGTCCTGTTGCGTTATCTCGCCCTGCCCTTAGCAGAAAACGGGCAGACCTTGTGGCTAG
GGTTAGACTCGTTAACTAACCTGAGCGCCTGTGAAGCCTTTTCTTTTCTCACCGGCAAAACCATTGAGCCGGTATTAATT
GAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTA
TTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCG
CGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTG
CTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATAT
TAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCA
CCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAACTGGCATTCGGTGAACTG
GGCATGACGGAATCACAGCAACAGCGTTTTCGTCGTGCCTTGAGCCAACCACAAGGCTTGATTTTAGTCACCGGTCCGAC
AGGCAGCGGCAAAAGTATTTCTTTATATACGGCGTTGCAGTACTTAAATACGTCTGAAAAACACATTATGACGGCGGAAG
ATCCCATTGAAATTCAGCTCCCAGGCATTATTCAAAGCCAAGTGAATCACTCTATCGGCTTGGATTTCAGCCGATTGCTT
CGCACCTTTTTACGCCAAGATCCGGACATTATTATGTTAGGTGAAATTCGTGATGAAGAAAGTGCCGCCATGGCATTACG
CGCCGCGCAAACGGGGCATTTGGTGCTTTCGACGCTACATACCAACGACGCCCCTTCCGCCATTTCCCGCCTGCAACAAC
TCGGCATTCAACGCCATGAAATCGACGGCAGTTTGTTGCTGGTGATCGCCCAACGTTTGGTGCGCAAACGCTGCCAAAAG
TGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACC
GAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCA
CAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-93)

Type II/IV secretion system protein (T2SSE)

(130-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

69.427

100

0.697
  pilB Haemophilus influenzae 86-028NP

70.87

98.081

0.695
  pilB Glaesserella parasuis strain SC1401

57.543

98.934

0.569
  pilB Vibrio cholerae strain A1552

40.53

100

0.424
  pilB Acinetobacter baylyi ADP1

39.078

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

40.041

100

0.412
  pilB Vibrio campbellii strain DS40M4

39.752

100

0.409
  pilB Acinetobacter baumannii D1279779

38.855

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.54

100

0.403
  pilF Neisseria gonorrhoeae MS11

36

100

0.365