Detailed information NTG0615420

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: LIN94_RS00655
• Genome accession: NZ_CP085092
• Coordinates: 128472..129881 (+)
• Length: 469 a.a.
• NCBI ID: WP_019518272.1
• Uniprot ID: Q9JRM9
• Organism: Aggregatibacter actinomycetemcomitans strain 30R
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 123472..134881

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LIN94_RS00635	pnp	123950..126079 (-)	2130	WP_025298076.1	polyribonucleotide nucleotidyltransferase	-
LIN94_RS00640	nanQ	126373..126843 (+)	471	WP_005547536.1	N-acetylneuraminate anomerase	-
LIN94_RS00645	ampD	127317..127871 (-)	555	WP_005567973.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
LIN94_RS00650	pilA	127993..128445 (+)	453	WP_249852904.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
LIN94_RS00655	pilB	128472..129881 (+)	1410	WP_019518272.1	GspE/PulE family protein	Machinery gene
LIN94_RS00660	pilC	129874..131097 (+)	1224	WP_025298077.1	type II secretion system F family protein	Machinery gene
LIN94_RS00665	-	131094..131783 (+)	690	WP_005547547.1	A24 family peptidase	-
LIN94_RS00670	coaE	131833..132456 (+)	624	WP_005547548.1	dephospho-CoA kinase	-
LIN94_RS00675	yacG	132446..132658 (+)	213	WP_005567966.1	DNA gyrase inhibitor YacG	-
LIN94_RS00680	-	132658..132930 (+)	273	WP_005547552.1	GNAT family N-acetyltransferase	-
LIN94_RS00685	-	133327..134700 (+)	1374	WP_005547554.1	sodium:alanine symporter family protein	-

Sequence

Protein

• Length: 469 a.a.
• Molecular weight: 52549.85 Da
• Isoelectric Point: 6.2867

>NTDB_id=615420 LIN94_RS00655 WP_019518272.1 128472..129881(+) (pilB) [Aggregatibacter actinomycetemcomitans strain 30R]
MQNPMAQGYLIHSQNHGDFTIAPELWLKNQQQQTVLLRYLALPLAENGQTLWLGLDSLTNLSACEAFSFLTGKTIEPVLI
ESQTLKTALQDLSPRVEKVEENQPLFYSALSQEQPTQPYDEPIIRLLNDIFESATAQKASDIHLEPQADCLQTRFRIDGV
LQVQNTVPLSIASRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDTLDFRLSTLATQFGEKAVLRLQQNRPVQLAFGEL
GMTESQQQRFRRALSQPQGLILVTGPTGSGKSISLYTALQYLNTSEKHIMTAEDPIEIQLPGIIQSQVNHPIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQRHEIDGSLLLVIAQRLVRKRCQK
CGGNATRFCDCHQGYKGRVGVYQFLQPNLQHPQGYETDFADLGQSALEKLKDGTTDLTEIQRVLGQTHD

Nucleotide

• Length: 1410 bp

>NTDB_id=615420 LIN94_RS00655 WP_019518272.1 128472..129881(+) (pilB) [Aggregatibacter actinomycetemcomitans strain 30R]
ATGCAAAATCCGATGGCGCAAGGTTATTTGATTCACAGCCAAAATCACGGGGACTTTACCATTGCGCCCGAGCTATGGCT
TAAAAATCAGCAACAACAAACCGTCCTGTTGCGTTATCTCGCCCTGCCCTTAGCAGAAAACGGGCAGACCTTGTGGCTAG
GGTTAGACTCGTTAACTAACCTGAGCGCCTGTGAAGCCTTTTCTTTTCTCACCGGCAAAACCATTGAGCCGGTATTAATT
GAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTA
TTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCG
CGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTG
CTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATAT
TAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCA
CCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAACTGGCATTCGGTGAACTG
GGCATGACGGAATCACAGCAACAGCGTTTTCGTCGTGCCTTGAGCCAACCACAAGGCTTGATTTTAGTCACCGGTCCGAC
AGGCAGCGGCAAAAGTATTTCTTTATATACGGCGTTGCAGTACTTAAATACGTCTGAAAAACACATTATGACGGCGGAAG
ATCCCATTGAAATTCAGCTCCCAGGCATTATTCAAAGCCAAGTGAATCACCCTATCGGCTTGGATTTCAGCCGATTGCTT
CGCACCTTTTTACGCCAAGATCCGGACATTATTATGTTAGGTGAAATTCGTGATGAAGAAAGTGCCGCCATGGCATTACG
CGCCGCGCAAACGGGGCATTTGGTGCTTTCGACGCTACATACCAACGACGCCCCTTCCGCCATTTCCCGCCTGCAACAAC
TCGGCATTCAACGCCATGAAATCGACGGCAGTTTGTTGCTGGTGATCGCCCAACGTTTGGTGCGCAAACGCTGCCAAAAG
TGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACC
GAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCA
CAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (130-396)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (31-93)

3D structure

• AlphaFold DB: Q9JRM9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Haemophilus influenzae Rd KW20	69.427	100	0.697
pilB	Haemophilus influenzae 86-028NP	70.87	98.081	0.695
pilB	Glaesserella parasuis strain SC1401	57.328	98.934	0.567
pilB	Vibrio cholerae strain A1552	40.53	100	0.424
pilB	Acinetobacter baylyi ADP1	39.078	100	0.416
pilB	Legionella pneumophila strain ERS1305867	39.468	100	0.412
pilB	Vibrio parahaemolyticus RIMD 2210633	40.041	100	0.412
pilB	Vibrio campbellii strain DS40M4	39.752	100	0.409
pilB	Acinetobacter baumannii D1279779	38.855	100	0.405
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.331	100	0.401
pilF	Neisseria gonorrhoeae MS11	36	100	0.365