Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LG951_RS17125 Genome accession   NZ_CP085037
Coordinates   3276486..3277613 (-) Length   375 a.a.
NCBI ID   WP_226804192.1    Uniprot ID   -
Organism   Bacillus pumilus strain BIM B-171     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3271486..3282613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG951_RS17090 (LG951_17090) flgL 3271666..3272577 (-) 912 WP_137054345.1 flagellar hook-associated protein FlgL -
  LG951_RS17095 (LG951_17095) flgK 3272587..3274107 (-) 1521 WP_226804189.1 flagellar hook-associated protein FlgK -
  LG951_RS17100 (LG951_17100) - 3274123..3274605 (-) 483 WP_050944342.1 flagellar protein FlgN -
  LG951_RS17105 (LG951_17105) flgM 3274624..3274887 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  LG951_RS17110 (LG951_17110) - 3274967..3275386 (-) 420 WP_003212865.1 TIGR03826 family flagellar region protein -
  LG951_RS17115 (LG951_17115) comFC 3275443..3276129 (-) 687 WP_226804191.1 ComF family protein Machinery gene
  LG951_RS17120 (LG951_17120) - 3276126..3276419 (-) 294 WP_050944343.1 late competence development ComFB family protein -
  LG951_RS17125 (LG951_17125) comFA 3276486..3277613 (-) 1128 WP_226804192.1 DEAD/DEAH box helicase Machinery gene
  LG951_RS17130 (LG951_17130) - 3277985..3278827 (-) 843 WP_226804196.1 DegV family protein -
  LG951_RS17135 (LG951_17135) degU 3279045..3279734 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  LG951_RS17140 (LG951_17140) degS 3279799..3280974 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  LG951_RS17145 (LG951_17145) - 3281196..3281864 (+) 669 WP_003212936.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42796.14 Da        Isoelectric Point: 10.4499

>NTDB_id=614842 LG951_RS17125 WP_226804192.1 3276486..3277613(-) (comFA) [Bacillus pumilus strain BIM B-171]
MMGKTTECGFLYEWTGPQMEEACHSELTWQGNLSKGQKRASEKLIEAIKNKSDLLIWAVCGAGKTEVLFHGIEYALHQGM
SVCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIAPLVIATTHQLMRYKHAFDVLIIDEVDAFPYSIDERLQF
AVLKAMRKKGVRVYLSATPSKKMISDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNWMQKHITK
KRRVLLFVPSISTMKKVTKVLRKHHLNVEGVSADDPDRKQKVQQFRDYKYDVLVTTTILERGVTISNVQVGVLGAESTIF
TESALVQISGRAGRHPDFFKGDVFFFHFGLTRSMKQAKKHIVKMNDTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=614842 LG951_RS17125 WP_226804192.1 3276486..3277613(-) (comFA) [Bacillus pumilus strain BIM B-171]
ATGATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAGCATGTCACTCGGAATT
AACATGGCAGGGAAACCTGTCTAAAGGACAAAAAAGAGCGTCAGAGAAATTGATCGAAGCGATAAAAAACAAATCTGATC
TACTCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATAGAATATGCCTTACATCAAGGAATG
AGTGTCTGCATTGCGACACCTAGAACTGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCTTTTCAAGGAATGAA
AATAGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGCACCGTTAGTAATCGCTACAACTCACCAGTTGATGA
GATATAAACATGCATTTGATGTGCTCATTATAGATGAAGTCGACGCCTTCCCTTATTCAATCGATGAACGTCTCCAATTT
GCTGTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCGTCTAAGAAAATGATAAGTGATGT
ATCTCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCTTTACCGGTCCCATCCTTTCAATGGA
TTGGACATTGGAAGAAGAAATTAAAAAAGAATCAGCTCCCACCTAAAGTAATGAATTGGATGCAGAAACATATTACAAAA
AAGAGAAGGGTTTTACTTTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACGAAGGTTCTTAGAAAGCACCACTTAAA
TGTAGAAGGGGTGTCTGCTGATGATCCAGATAGGAAACAAAAGGTCCAGCAATTCAGAGATTACAAATACGATGTACTAG
TTACAACCACAATTCTAGAAAGAGGTGTAACCATTTCAAATGTACAAGTGGGGGTACTAGGTGCAGAATCTACTATCTTT
ACAGAAAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCCCGATTTTTTTAAAGGAGATGTTTTCTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATTGTAAAAATGAATGATACAGCTGAAAAAGAGTTCTCCG
AAAAATAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

58.108

98.667

0.573
  comFA Latilactobacillus sakei subsp. sakei 23K

38.978

99.2

0.387
  comFA/cflA Streptococcus mitis SK321

40.395

94.4

0.381
  comFA/cflA Streptococcus pneumoniae Rx1

40.113

94.4

0.379
  comFA/cflA Streptococcus pneumoniae D39

40.113

94.4

0.379
  comFA/cflA Streptococcus pneumoniae R6

40.113

94.4

0.379
  comFA/cflA Streptococcus pneumoniae TIGR4

39.831

94.4

0.376
  comFA/cflA Streptococcus mitis NCTC 12261

39.266

94.4

0.371
  comFA Lactococcus lactis subsp. cremoris KW2

38.162

95.733

0.365