Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LEO79_RS13400 Genome accession   NZ_CP084350
Coordinates   2868602..2869711 (-) Length   369 a.a.
NCBI ID   WP_226043715.1    Uniprot ID   -
Organism   Aeromonas caviae strain 71442     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2863602..2874711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO79_RS13375 (LEO79_13375) - 2863734..2863961 (+) 228 WP_039039956.1 hypothetical protein -
  LEO79_RS13380 (LEO79_13380) brnQ 2864052..2865314 (-) 1263 WP_045523295.1 branched-chain amino acid transport system II carrier protein -
  LEO79_RS13385 (LEO79_13385) - 2865550..2866260 (-) 711 WP_226043714.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  LEO79_RS13390 (LEO79_13390) srmB 2866388..2867608 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  LEO79_RS13395 (LEO79_13395) yaaA 2867796..2868569 (-) 774 WP_045523231.1 peroxide stress protein YaaA -
  LEO79_RS13400 (LEO79_13400) pilU 2868602..2869711 (-) 1110 WP_226043715.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LEO79_RS13405 (LEO79_13405) pilT 2869735..2870769 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  LEO79_RS13410 (LEO79_13410) - 2870809..2871510 (+) 702 WP_049636471.1 YggS family pyridoxal phosphate-dependent enzyme -
  LEO79_RS13415 (LEO79_13415) proC 2871633..2872457 (+) 825 WP_219604084.1 pyrroline-5-carboxylate reductase -
  LEO79_RS13420 (LEO79_13420) - 2872481..2873032 (+) 552 WP_042014922.1 YggT family protein -
  LEO79_RS13425 (LEO79_13425) yggU 2873032..2873331 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  LEO79_RS13430 (LEO79_13430) - 2873350..2873769 (+) 420 WP_041210940.1 DUF4426 domain-containing protein -
  LEO79_RS13435 (LEO79_13435) - 2873845..2874675 (-) 831 WP_226043716.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40931.07 Da        Isoelectric Point: 6.3155

>NTDB_id=610731 LEO79_RS13400 WP_226043715.1 2868602..2869711(-) (pilU) [Aeromonas caviae strain 71442]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMTKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=610731 LEO79_RS13400 WP_226043715.1 2868602..2869711(-) (pilU) [Aeromonas caviae strain 71442]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAAA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGACCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CAGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.56

91.057

0.388
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379