Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RBAU_RS12415 Genome accession   NC_022075
Coordinates   2591776..2592846 (-) Length   356 a.a.
NCBI ID   WP_007408320.1    Uniprot ID   -
Organism   Bacillus velezensis UCMB5033     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2586776..2597846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBAU_RS12365 (RBAU_2431) - 2586836..2587420 (-) 585 WP_007408328.1 signal peptidase I -
  RBAU_RS12370 (RBAU_2432) tapA 2587392..2588063 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  RBAU_RS12375 (RBAU_2433) - 2588322..2588651 (+) 330 WP_020954231.1 DUF3889 domain-containing protein -
  RBAU_RS12380 (RBAU_2434) - 2588691..2588870 (-) 180 WP_003153093.1 YqzE family protein -
  RBAU_RS12385 (RBAU_2435) comGG 2588927..2589304 (-) 378 WP_007408325.1 competence type IV pilus minor pilin ComGG Machinery gene
  RBAU_RS12390 (RBAU_2436) comGF 2589305..2589805 (-) 501 WP_258566475.1 competence type IV pilus minor pilin ComGF -
  RBAU_RS12395 (RBAU_2437) comGE 2589714..2590028 (-) 315 WP_007408323.1 competence type IV pilus minor pilin ComGE -
  RBAU_RS12400 (RBAU_2438) comGD 2590012..2590449 (-) 438 WP_007408322.1 competence type IV pilus minor pilin ComGD Machinery gene
  RBAU_RS12405 (RBAU_2439) comGC 2590439..2590705 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  RBAU_RS12410 (RBAU_2440) comGB 2590752..2591789 (-) 1038 WP_007408321.1 competence type IV pilus assembly protein ComGB Machinery gene
  RBAU_RS12415 (RBAU_2441) comGA 2591776..2592846 (-) 1071 WP_007408320.1 competence type IV pilus ATPase ComGA Machinery gene
  RBAU_RS12420 (RBAU_2442) - 2593038..2593988 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  RBAU_RS12425 (RBAU_2443) - 2594134..2595435 (+) 1302 WP_007408318.1 hemolysin family protein -
  RBAU_RS12430 (RBAU_2444) - 2595473..2596303 (-) 831 WP_007408317.1 STAS domain-containing protein -
  RBAU_RS12440 (RBAU_2445) - 2596510..2596890 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RBAU_RS12445 (RBAU_2446) - 2597127..2597372 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.79 Da        Isoelectric Point: 9.1996

>NTDB_id=60993 RBAU_RS12415 WP_007408320.1 2591776..2592846(-) (comGA) [Bacillus velezensis UCMB5033]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=60993 RBAU_RS12415 WP_007408320.1 2591776..2592846(-) (comGA) [Bacillus velezensis UCMB5033]
GTGGAAAATATTGAAACATACAGCAGGAACATCATAAATGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTTTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACTACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GACAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment