Detailed information NTG0060993

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: EL277_RS06225
• Genome accession: NZ_AP018936
• Coordinates: 1195162..1196010 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705302.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain NU83127
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 1190162..1201010

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL277_RS06210 (SPNU_1185)	-	1191990..1193033 (-)	1044	WP_001812710.1	TIGR00341 family protein	-
EL277_RS06215 (SPNU_1186)	-	1193637..1194590 (+)	954	WP_001155321.1	IS30-like element ISSpn8 family transposase	-
EL277_RS06225 (SPNU_1187)	dprA/cilB/dalA	1195162..1196010 (-)	849	WP_000705302.1	DNA-processing protein DprA	Machinery gene
EL277_RS06230 (SPNU_1188)	-	1196107..1196796 (-)	690	WP_000643970.1	sugar phosphate nucleotidyltransferase	-
EL277_RS06235 (SPNU_1189)	-	1196808..1197686 (-)	879	WP_000837609.1	DMT family transporter	-
EL277_RS06240 (SPNU_1190)	licA	1197676..1198545 (-)	870	WP_000411205.1	choline kinase LicA	-
EL277_RS06245 (SPNU_1191)	-	1198562..1199584 (-)	1023	WP_000609894.1	ribitol-5-phosphate dehydrogenase	-
EL277_RS06250 (SPNU_1192)	-	1199589..1200296 (-)	708	WP_000638501.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31090.78 Da
• Isoelectric Point: 5.4047

>NTDB_id=60993 EL277_RS06225 WP_000705302.1 1195162..1196010(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain NU83127]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPASILDGLSDGCHHLIQEGAKLLTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=60993 EL277_RS06225 WP_000705302.1 1195162..1196010(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain NU83127]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGCTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGCTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterProScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	99.291	100	0.993
dprA/cilB/dalA	Streptococcus pneumoniae D39	99.291	100	0.993
dprA/cilB/dalA	Streptococcus pneumoniae R6	99.291	100	0.993
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	99.291	100	0.993
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	97.872	100	0.979
dprA/cilB/dalA	Streptococcus mitis SK321	97.163	100	0.972
dprA	Streptococcus mutans UA159	62.857	99.291	0.624
dprA	Lactococcus lactis subsp. cremoris KW2	55	99.291	0.546
dprA	Staphylococcus aureus N315	37.102	100	0.372