Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JF568_RS00095 Genome accession   NZ_CP083931
Coordinates   18235..19416 (-) Length   393 a.a.
NCBI ID   WP_097113156.1    Uniprot ID   -
Organism   Alysiella filiformis DSM 16848     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 13235..24416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JF568_RS00075 (JF568_00075) - 13509..14954 (-) 1446 WP_097113160.1 cell wall metabolism sensor histidine kinase WalK -
  JF568_RS00080 (JF568_00080) - 15091..15774 (-) 684 WP_097113159.1 response regulator transcription factor -
  JF568_RS00085 (JF568_00085) - 15927..17795 (-) 1869 WP_097113158.1 PglL family O-oligosaccharyltransferase -
  JF568_RS00090 (JF568_00090) - 17792..18151 (-) 360 WP_097113157.1 DUF2069 domain-containing protein -
  JF568_RS00095 (JF568_00095) pilU 18235..19416 (-) 1182 WP_097113156.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JF568_RS00100 (JF568_00100) - 19563..21083 (-) 1521 WP_097113155.1 acetyl-CoA hydrolase/transferase family protein -
  JF568_RS00105 (JF568_00105) - 21154..21327 (+) 174 WP_179655751.1 hypothetical protein -
  JF568_RS00110 (JF568_00110) - 21346..22530 (+) 1185 WP_097113154.1 acetylornithine/succinyldiaminopimelate transaminase -
  JF568_RS00115 (JF568_00115) - 22590..23240 (-) 651 WP_097113153.1 YitT family protein -
  JF568_RS00120 (JF568_00120) - 23439..23681 (-) 243 WP_034290658.1 hypothetical protein -
  JF568_RS00125 (JF568_00125) - 23749..24021 (-) 273 WP_143269078.1 hypothetical protein -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43809.18 Da        Isoelectric Point: 5.4709

>NTDB_id=608574 JF568_RS00095 WP_097113156.1 18235..19416(-) (pilU) [Alysiella filiformis DSM 16848]
MSAVNIPPINEEMKKFIHGLLTHMVKNKGSDLFITAGYPPAMKLDGKLTKITDKPLTAEHTQQIARSIMDDKQAEEFFST
NECNFAISLAGVSRFRINAMVQRGATALVCRVITSDIPKFENMNLPPVLKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDY
RNANSYGHIITIEDPIEFVHPHQNCIITQREVGVDTQDWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMA
TLHANNSNQALDRIINFFPEERRNQLLNDLSLNLKGFISQRLIPKKEGKGRCAAVEILLNSPLISELILRGDIHGVKEIM
AKSRDIGMQTFDQSLFDLYESDLISYDEALKNADSVNDLRLQIQLNSKKNTTAGSGNVLDSLALTEFEEPEEE

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=608574 JF568_RS00095 WP_097113156.1 18235..19416(-) (pilU) [Alysiella filiformis DSM 16848]
ATGTCCGCAGTAAACATTCCACCGATTAACGAAGAAATGAAAAAATTCATTCATGGCTTGCTCACGCACATGGTGAAAAA
CAAGGGTTCGGACTTGTTCATTACCGCAGGCTATCCGCCCGCCATGAAATTGGACGGCAAACTGACCAAAATTACCGACA
AACCGCTTACCGCCGAACACACCCAGCAAATCGCCCGTTCCATTATGGACGACAAACAAGCGGAAGAATTTTTCAGCACC
AATGAATGCAATTTTGCGATTAGTTTGGCAGGCGTATCGCGTTTCCGCATTAATGCGATGGTACAGCGTGGCGCAACCGC
ACTCGTTTGCCGCGTGATTACCAGCGACATTCCCAAATTTGAAAACATGAATTTGCCACCTGTGTTGAAACAAGTGGTTA
TGGAAAAACGCGGTTTGGTGATTTTCGTGGGCGGTACGGGTTCGGGTAAATCCACGTCTTTGGCGGCGATGATTGACTAC
CGCAATGCCAACAGCTACGGACACATCATCACGATTGAAGACCCGATTGAATTTGTTCACCCACACCAAAATTGCATCAT
CACGCAACGCGAAGTGGGCGTGGACACGCAAGACTGGTTTGCCGCGCTGAAAAACACTTTGCGCCAAGCCCCTGATGTGA
TTTTGATTGGCGAAATCCGCGACCGCGAAACCATGGATTATGCCCTTGCCTTTGCCGAAACAGGGCATTTGTGTATGGCA
ACGCTGCACGCGAATAACTCCAATCAAGCACTTGACCGCATTATTAACTTTTTCCCCGAAGAACGCCGCAATCAGTTGTT
GAATGATTTGTCTTTGAATTTAAAGGGTTTCATTTCTCAACGTCTGATTCCCAAAAAAGAAGGCAAGGGACGCTGTGCGG
CGGTGGAAATTTTGCTCAATTCGCCCCTGATTTCCGAATTGATTTTGCGTGGCGACATTCATGGCGTGAAAGAAATCATG
GCAAAATCGCGCGATATTGGTATGCAAACGTTTGACCAATCGCTGTTTGATTTGTATGAATCCGATTTGATTAGCTACGA
TGAGGCTTTGAAAAATGCTGATTCGGTCAATGACTTGCGTTTGCAAATCCAACTGAACAGCAAGAAAAACACCACCGCAG
GTTCGGGTAATGTGCTGGACAGTTTGGCTTTGACCGAATTTGAAGAGCCAGAAGAAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.544

89.313

0.532
  pilU Acinetobacter baylyi ADP1

58.523

89.567

0.524
  pilU Vibrio cholerae strain A1552

53.406

93.384

0.499
  pilT Legionella pneumophila strain Lp02

43.62

85.751

0.374
  pilT Legionella pneumophila strain ERS1305867

43.62

85.751

0.374
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.072

84.478

0.364