Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JF634_RS10985 Genome accession   NZ_CP083930
Coordinates   2209319..2210512 (+) Length   397 a.a.
NCBI ID   WP_002642455.1    Uniprot ID   V9HC56
Organism   Simonsiella muelleri strain DSM 2579     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2204319..2215512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JF634_RS10950 (JF634_10950) - 2204666..2204854 (-) 189 WP_002642462.1 DUF3460 family protein -
  JF634_RS10955 (JF634_10955) pth 2204908..2205480 (-) 573 WP_002642461.1 aminoacyl-tRNA hydrolase -
  JF634_RS10960 (JF634_10960) - 2205488..2205760 (-) 273 WP_002642460.1 RnfH family protein -
  JF634_RS10965 (JF634_10965) - 2205753..2206184 (-) 432 WP_002642459.1 type II toxin-antitoxin system RatA family toxin -
  JF634_RS10970 (JF634_10970) - 2206402..2207211 (-) 810 WP_002642458.1 basic amino acid ABC transporter substrate-binding protein -
  JF634_RS10975 (JF634_10975) folE2 2207325..2208095 (-) 771 WP_002642457.1 GTP cyclohydrolase FolE2 -
  JF634_RS10980 (JF634_10980) - 2208228..2209022 (-) 795 WP_002642456.1 inositol monophosphatase family protein -
  JF634_RS10985 (JF634_10985) pilU 2209319..2210512 (+) 1194 WP_002642455.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JF634_RS10990 (JF634_10990) - 2210591..2210953 (+) 363 WP_002642454.1 DUF2069 domain-containing protein -
  JF634_RS10995 (JF634_10995) rlmB 2211034..2211783 (+) 750 WP_002642453.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  JF634_RS11000 (JF634_11000) - 2212100..2212864 (+) 765 WP_002642452.1 formylglycine-generating enzyme family protein -
  JF634_RS11005 (JF634_11005) ubiB 2212984..2214495 (+) 1512 WP_002642451.1 ubiquinone biosynthesis regulatory protein kinase UbiB -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 44363.90 Da        Isoelectric Point: 6.1895

>NTDB_id=608572 JF634_RS10985 WP_002642455.1 2209319..2210512(+) (pilU) [Simonsiella muelleri strain DSM 2579]
MSKVHIPPVNEEMKKFIHNLLTHMFKNKGSDLFITAGYPPAMKLDGKLTKITDKPLTAEHTEQIARSIMDDKQAEEFFGT
NECNFAISLAGVSRFRVNAMVQRGAAALVCRVITSDIPKFDNMNLPPILKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDY
RNANSYGHIITIEDPIEFVHPHQNCIITQREVGVDTADWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMA
TLHANNSNQALDRIINFFPEERRSQLLNDLSLNLKGFISQRLIPKAEGKGRVAAVEVLLNSPLISELILRGDIHAVKEIM
AKSRDIGMQTFDQSLFDLYEENLITYEEALKNADSVNDLRLQIQLNSKKNKTAGSDGVLDNLFLTDYEEPEDGTKKK

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=608572 JF634_RS10985 WP_002642455.1 2209319..2210512(+) (pilU) [Simonsiella muelleri strain DSM 2579]
ATGTCAAAAGTACACATCCCACCCGTAAATGAGGAAATGAAAAAATTCATTCACAATTTATTAACGCATATGTTCAAAAA
TAAAGGTTCGGATTTATTCATCACGGCAGGTTATCCACCAGCAATGAAATTGGACGGCAAATTGACCAAAATCACCGATA
AGCCCTTGACCGCCGAGCATACTGAACAAATCGCTCGTTCTATTATGGACGACAAACAAGCCGAAGAATTTTTTGGGACA
AACGAATGTAATTTTGCAATTAGTTTGGCGGGTGTATCGCGTTTTCGTGTGAATGCAATGGTGCAACGTGGAGCGGCGGC
GTTGGTGTGTCGGGTGATTACCAGCGATATTCCAAAATTTGACAACATGAATTTGCCACCGATTTTGAAACAGGTTGTGA
TGGAGAAACGTGGTTTGGTGATTTTTGTGGGTGGTACGGGTTCGGGTAAATCTACGTCGTTGGCAGCGATGATTGACTAT
CGCAACGCCAACAGCTACGGACACATCATTACCATTGAAGACCCAATTGAGTTCGTTCATCCGCATCAAAATTGCATCAT
CACACAACGTGAAGTGGGCGTGGATACGGCAGATTGGTTTGCAGCACTGAAAAACACCCTGCGTCAAGCACCTGATGTGA
TTTTGATTGGTGAGATTCGCGACCGCGAAACCATGGATTATGCGTTGGCGTTTGCGGAAACGGGGCATTTGTGTATGGCA
ACGTTGCACGCGAATAATTCTAACCAAGCACTTGATCGCATCATCAACTTTTTCCCAGAAGAACGCCGCTCCCAATTGTT
GAATGATTTATCATTGAATTTGAAAGGTTTTATTTCGCAACGTCTTATTCCCAAAGCCGAAGGCAAGGGGCGCGTGGCGG
CGGTGGAGGTGTTGCTCAATTCACCGTTGATTTCGGAGTTGATTTTGCGTGGCGACATTCATGCTGTGAAAGAAATCATG
GCAAAATCGCGTGATATTGGTATGCAAACATTTGACCAATCGTTGTTTGATTTGTATGAAGAAAATTTGATTACTTATGA
AGAAGCTTTGAAAAATGCGGATTCTGTCAATGACTTGCGCTTGCAAATTCAATTGAACAGTAAGAAAAATAAAACCGCGG
GTTCAGATGGCGTGTTGGACAATTTGTTTTTGACTGATTACGAAGAACCCGAAGATGGAACCAAGAAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(126-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V9HC56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

59.322

89.169

0.529
  pilU Pseudomonas stutzeri DSM 10701

59.829

88.413

0.529
  pilU Vibrio cholerae strain A1552

52.291

93.451

0.489
  pilT Legionella pneumophila strain Lp02

44.444

83.879

0.373
  pilT Legionella pneumophila strain ERS1305867

44.444

83.879

0.373
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.027

84.887

0.365