Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NMD30_RS03675 Genome accession   NZ_CP100994
Coordinates   752910..754403 (+) Length   497 a.a.
NCBI ID   WP_000249355.1    Uniprot ID   -
Organism   Escherichia coli strain ET201     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 747910..759403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD30_RS03655 (NMD30_03645) pilD 748558..749367 (+) 810 WP_000895884.1 prepilin peptidase PppA Machinery gene
  NMD30_RS03660 (NMD30_03650) gspS2 749433..749843 (+) 411 WP_001298744.1 type II secretion system pilot lipoprotein GspS-beta -
  NMD30_RS03665 (NMD30_03655) gspC 749861..750820 (+) 960 WP_001307390.1 type II secretion system protein GspC -
  NMD30_RS03670 (NMD30_03660) gspD 750850..752910 (+) 2061 WP_000498836.1 type II secretion system secretin GspD -
  NMD30_RS03675 (NMD30_03665) pilB/pilB1 752910..754403 (+) 1494 WP_000249355.1 type II secretion system ATPase GspE Machinery gene
  NMD30_RS03680 (NMD30_03670) gspF 754403..755626 (+) 1224 WP_085668592.1 type II secretion system inner membrane protein GspF -
  NMD30_RS03685 (NMD30_03675) gspG 755643..756098 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  NMD30_RS03690 (NMD30_03680) gspH 756102..756665 (+) 564 WP_001115138.1 type II secretion system minor pseudopilin GspH -
  NMD30_RS03695 (NMD30_03685) gspI 756662..757033 (+) 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
  NMD30_RS03700 (NMD30_03690) gspJ 757030..757635 (+) 606 WP_033805213.1 type II secretion system minor pseudopilin GspJ -
  NMD30_RS03705 (NMD30_03695) gspK 757632..758609 (+) 978 WP_000633193.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55206.37 Da        Isoelectric Point: 6.0390

>NTDB_id=608326 NMD30_RS03675 WP_000249355.1 752910..754403(+) (pilB/pilB1) [Escherichia coli strain ET201]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=608326 NMD30_RS03675 WP_000249355.1 752910..754403(+) (pilB/pilB1) [Escherichia coli strain ET201]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGTTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGATGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACA
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGACCGACGGGTTCCGGCAAAAGCACTAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGAGACTGGTCCGCACGCTGTGTCCCGACTGCCGCCAGGCTGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilF Thermus thermophilus HB27

45

80.483

0.362
  pilB Acinetobacter baylyi ADP1

45.897

78.471

0.36