Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   NMD30_RS03655 Genome accession   NZ_CP100994
Coordinates   748558..749367 (+) Length   269 a.a.
NCBI ID   WP_000895884.1    Uniprot ID   -
Organism   Escherichia coli strain ET201     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 743558..754367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD30_RS03640 (NMD30_03630) gss 744010..745869 (-) 1860 WP_001297309.1 bifunctional glutathionylspermidine amidase/synthase -
  NMD30_RS03645 (NMD30_03635) yghU 746074..746940 (+) 867 WP_001298746.1 glutathione-dependent disulfide-bond oxidoreductase -
  NMD30_RS03650 (NMD30_03640) - 747240..748219 (+) 980 Protein_716 IS110-like element IS621 family transposase -
  NMD30_RS03655 (NMD30_03645) pilD 748558..749367 (+) 810 WP_000895884.1 prepilin peptidase PppA Machinery gene
  NMD30_RS03660 (NMD30_03650) gspS2 749433..749843 (+) 411 WP_001298744.1 type II secretion system pilot lipoprotein GspS-beta -
  NMD30_RS03665 (NMD30_03655) gspC 749861..750820 (+) 960 WP_001307390.1 type II secretion system protein GspC -
  NMD30_RS03670 (NMD30_03660) gspD 750850..752910 (+) 2061 WP_000498836.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29508.22 Da        Isoelectric Point: 8.3829

>NTDB_id=608325 NMD30_RS03655 WP_000895884.1 748558..749367(+) (pilD) [Escherichia coli strain ET201]
MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAI
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=608325 NMD30_RS03655 WP_000895884.1 748558..749367(+) (pilD) [Escherichia coli strain ET201]
ATGCTTTTTGATGTTTTTCAGCAATACCCCGTGGCGATGCCCATACTGGCTACCGTCGGAGGATTAATCATCGGTAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGAGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGGCGCAACAGAGTCCGTTAACGCTACAAGACGCAGTTACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTGGCTGGGTGGGGCCGTTATCACTGCCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCATT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-240)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.693

99.257

0.454
  pilD Vibrio campbellii strain DS40M4

42.066

100

0.424
  pilD Acinetobacter nosocomialis M2

38.868

98.513

0.383
  pilD Acinetobacter baumannii D1279779

38.491

98.513

0.379
  pilD Neisseria gonorrhoeae MS11

39.689

95.539

0.379