Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LA321_RS09260 Genome accession   NZ_CP083625
Coordinates   1969063..1970172 (+) Length   369 a.a.
NCBI ID   WP_042070431.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain FDAARGOS_1510     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1964063..1975172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA321_RS09230 (LA321_09230) - 1965066..1965485 (-) 420 WP_026457681.1 DUF4426 domain-containing protein -
  LA321_RS09235 (LA321_09235) yggU 1965522..1965815 (-) 294 WP_042070436.1 DUF167 family protein YggU -
  LA321_RS09240 (LA321_09240) - 1965815..1966366 (-) 552 WP_026457844.1 YggT family protein -
  LA321_RS09245 (LA321_09245) proC 1966378..1967202 (-) 825 WP_042070434.1 pyrroline-5-carboxylate reductase -
  LA321_RS09250 (LA321_09250) - 1967259..1967960 (-) 702 WP_042070432.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA321_RS09255 (LA321_09255) pilT 1968000..1969034 (+) 1035 WP_026457847.1 type IVa pilus ATPase TapT Machinery gene
  LA321_RS09260 (LA321_09260) pilU 1969063..1970172 (+) 1110 WP_042070431.1 type IVa pilus ATPase TapU Machinery gene
  LA321_RS09265 (LA321_09265) yaaA 1970216..1970989 (+) 774 WP_042070430.1 peroxide stress protein YaaA -
  LA321_RS09270 (LA321_09270) - 1971179..1971604 (+) 426 WP_042026650.1 hypothetical protein -
  LA321_RS09275 (LA321_09275) - 1971959..1972450 (-) 492 WP_042070429.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LA321_RS09280 (LA321_09280) arfB 1972481..1972897 (-) 417 WP_026456194.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  LA321_RS09285 (LA321_09285) glnK 1973099..1973437 (+) 339 WP_026456195.1 P-II family nitrogen regulator -
  LA321_RS09290 (LA321_09290) - 1973568..1974047 (-) 480 WP_042070428.1 MarR family winged helix-turn-helix transcriptional regulator -
  LA321_RS09295 (LA321_09295) - 1974207..1974734 (+) 528 WP_042070426.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41199.19 Da        Isoelectric Point: 6.2710

>NTDB_id=606746 LA321_RS09260 WP_042070431.1 1969063..1970172(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1510]
MNLDQMLTELVSRKGSDLFVTVGSPPTLKVNGHLVALGNEPLDKKEALTLVRETLSSDHFERYIRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDDLSLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRGIVAQQLVPSIDGRRRFAAFEILLNTPLITDIIRKGEMHRLKEVMTKSSELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGHEQLGSGMLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=606746 LA321_RS09260 WP_042070431.1 1969063..1970172(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1510]
ATGAATCTGGATCAGATGCTGACCGAACTGGTCAGCCGCAAGGGCTCGGATCTGTTTGTGACCGTGGGCTCGCCCCCCAC
CCTCAAGGTCAATGGTCACCTGGTGGCACTGGGCAACGAGCCCCTCGACAAGAAGGAGGCGCTGACCCTGGTCAGGGAGA
CCCTCAGCTCGGACCATTTTGAACGCTACATCCGAACCAAGGAGGCCAACTACGCCATCCATCGCGAGGAGCTGGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAAGAGCTGCCTGGCATGGTGCTGCGCCGCATCGAGACTCGCATTCCCACCTTCGA
TGATCTGAGCCTGCCTCCCATATTGCAGGACGTGGCGATGGCCAAGCGGGGACTGGTGTTGTTCGTCGGGGCCACGGGGG
CGGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGCGGATGGGCACATACTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCACCAACACGGCCGCAGCCTGGTGACTCAGCGCGAGGTGGGGATAGACACCGAATCGTTCGACGT
GGCGCTGAAGAGCTCTCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACTGGTCACCTCTGTCTGGCAACTCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAACACCGCCAGTTTCTGTTCGATCTCTCGTTCAACCTGCGTGGCATCGTTGCCCAGCA
ACTGGTGCCCAGTATCGACGGACGGCGACGTTTTGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCACCGGCTCAAGGAAGTGATGACCAAATCCTCAGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTGCCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCATGCCGACTCGGCCAATGACCTGCGACT
GCTGATCAAGCTTTCGGGTCATGAACAGCTGGGGTCCGGCATGCTGGACAATGTGACGCTAGACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.689

95.122

0.558
  pilU Acinetobacter baylyi ADP1

56.091

95.664

0.537
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374