Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LA358_RS05185 Genome accession   NZ_CP083612
Coordinates   1077749..1078858 (+) Length   369 a.a.
NCBI ID   WP_224210982.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain FDAARGOS_1536     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072749..1083858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA358_RS05155 (LA358_05155) - 1073752..1074171 (-) 420 WP_026457681.1 DUF4426 domain-containing protein -
  LA358_RS05160 (LA358_05160) yggU 1074208..1074501 (-) 294 WP_224210979.1 DUF167 family protein YggU -
  LA358_RS05165 (LA358_05165) - 1074501..1075052 (-) 552 WP_026457844.1 YggT family protein -
  LA358_RS05170 (LA358_05170) proC 1075064..1075888 (-) 825 WP_224210980.1 pyrroline-5-carboxylate reductase -
  LA358_RS05175 (LA358_05175) - 1075945..1076646 (-) 702 WP_224210981.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA358_RS05180 (LA358_05180) pilT 1076686..1077720 (+) 1035 WP_026457847.1 type IVa pilus ATPase TapT Machinery gene
  LA358_RS05185 (LA358_05185) pilU 1077749..1078858 (+) 1110 WP_224210982.1 type IVa pilus ATPase TapU Machinery gene
  LA358_RS05190 (LA358_05190) yaaA 1078902..1079675 (+) 774 WP_224210983.1 peroxide stress protein YaaA -
  LA358_RS05195 (LA358_05195) - 1079865..1080290 (+) 426 WP_042026650.1 hypothetical protein -
  LA358_RS05200 (LA358_05200) - 1080641..1081132 (-) 492 WP_224210984.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LA358_RS05205 (LA358_05205) arfB 1081162..1081578 (-) 417 WP_224210985.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  LA358_RS05210 (LA358_05210) glnK 1081780..1082118 (+) 339 WP_224210986.1 P-II family nitrogen regulator -
  LA358_RS05215 (LA358_05215) - 1082249..1082728 (-) 480 WP_224210987.1 MarR family winged helix-turn-helix transcriptional regulator -
  LA358_RS05220 (LA358_05220) - 1082888..1083415 (+) 528 WP_042070426.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41142.14 Da        Isoelectric Point: 6.2710

>NTDB_id=606628 LA358_RS05185 WP_224210982.1 1077749..1078858(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1536]
MNLDQMLAELVSRKGSDLFVTVGSPPTLKVNGHLVALGNEPLDKKEALTLVRETLSSDHFERYIRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDDLSLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSTETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRGIVAQQLVPSIDGRRRFAAFEILLNTPLITDIIRKGEMHRLKEVMTKSSELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGHEQLGSGMLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=606628 LA358_RS05185 WP_224210982.1 1077749..1078858(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1536]
ATGAATCTGGATCAGATGCTGGCCGAACTGGTCAGCCGCAAGGGCTCGGATCTGTTTGTAACCGTGGGCTCGCCCCCCAC
CCTCAAGGTCAATGGTCACCTGGTGGCACTGGGCAACGAGCCCCTCGACAAGAAGGAGGCGCTGACCCTGGTCAGGGAGA
CCCTCAGCTCGGACCATTTTGAACGCTACATCCGCACCAAGGAGGCCAACTACGCCATCCATCGCGAGGAGCTGGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGTTGCCTGGCATGGTGCTGCGCCGCATCGAGACTCGCATTCCCACCTTTGA
TGATCTGAGCCTGCCCCCCATATTGCAGGACGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTCGTCGGGGCCACGGGGG
CGGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGCGGATGGTCACATTCTGACAGTGGAAGAT
CCGGTGGAGTTCGTGCACCAGCACGGCCGCAGCCTGGTGACCCAGCGCGAGGTGGGGATTGACACCGAATCGTTTGACGT
GGCGCTGAAGAGCTCTCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAGATCCGAAGTACAGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGTCTGGCAACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTTCCCCAGGAGAAGCATCGCCAGTTCCTGTTCGATCTCTCGTTCAACCTGCGTGGCATCGTTGCCCAGCA
ACTGGTGCCCAGCATCGACGGGCGGCGACGTTTTGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGATATCA
TCCGCAAGGGAGAGATGCACCGGCTCAAGGAAGTGATGACCAAATCCTCAGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTGCCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCATGCCGACTCGGCCAATGATCTGCGACT
GCTTATCAAGCTCTCCGGTCATGAACAGCTGGGATCCGGCATGCTGGACAATGTGACGCTAGACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.689

95.122

0.558
  pilU Acinetobacter baylyi ADP1

56.091

95.664

0.537
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377