Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NLS69_RS27265 Genome accession   NZ_CP100653
Coordinates   5847033..5847656 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain F13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5842033..5852656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLS69_RS27245 (NLS69_27245) aroB 5842628..5843734 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  NLS69_RS27250 (NLS69_27250) aroK 5843784..5844302 (-) 519 WP_003095833.1 shikimate kinase AroK -
  NLS69_RS27255 (NLS69_27255) pilQ 5844314..5846458 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  NLS69_RS27260 (NLS69_27260) comL/pilP 5846512..5847036 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  NLS69_RS27265 (NLS69_27265) pilO 5847033..5847656 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  NLS69_RS27270 (NLS69_27270) pilN 5847653..5848249 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  NLS69_RS27275 (NLS69_27275) pilM 5848249..5849313 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  NLS69_RS27280 (NLS69_27280) - 5849498..5851969 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=605987 NLS69_RS27265 WP_003103268.1 5847033..5847656(-) (pilO) [Pseudomonas aeruginosa strain F13]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=605987 NLS69_RS27265 WP_003103268.1 5847033..5847656(-) (pilO) [Pseudomonas aeruginosa strain F13]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401