Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   K6V71_RS14460 Genome accession   NZ_CP083438
Coordinates   1191603..1192961 (+) Length   452 a.a.
NCBI ID   WP_420070544.1    Uniprot ID   -
Organism   Cupriavidus gilardii strain NOV2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1186603..1197961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6V71_RS14430 (K6V71_14365) proB 1186910..1188034 (-) 1125 WP_064577249.1 glutamate 5-kinase -
  K6V71_RS14435 (K6V71_14370) obgE 1188097..1189194 (-) 1098 WP_053822555.1 GTPase ObgE -
  K6V71_RS14440 (K6V71_14375) rpmA 1189332..1189592 (-) 261 WP_053822556.1 50S ribosomal protein L27 -
  K6V71_RS14445 (K6V71_14380) rplU 1189656..1189967 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  K6V71_RS14450 (K6V71_14385) ispB 1190511..1191440 (+) 930 WP_053822557.1 octaprenyl diphosphate synthase -
  K6V71_RS14460 (K6V71_14395) pilF 1191603..1192961 (+) 1359 WP_420070544.1 GspE/PulE family protein Machinery gene
  K6V71_RS14465 (K6V71_14400) - 1192995..1194119 (+) 1125 WP_263092647.1 type II secretion system F family protein -
  K6V71_RS14470 (K6V71_14405) - 1194121..1195062 (+) 942 WP_420070543.1 prepilin peptidase -
  K6V71_RS14475 (K6V71_14410) coaE 1195075..1195707 (+) 633 WP_053822559.1 dephospho-CoA kinase -
  K6V71_RS14480 (K6V71_14415) zapD 1195958..1196716 (+) 759 WP_006578256.1 cell division protein ZapD -
  K6V71_RS14485 (K6V71_14420) - 1196723..1196911 (+) 189 WP_053822560.1 DNA gyrase inhibitor YacG -
  K6V71_RS14490 (K6V71_14425) - 1196939..1197343 (-) 405 WP_053822561.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48762.40 Da        Isoelectric Point: 8.3207

>NTDB_id=605833 K6V71_RS14460 WP_420070544.1 1191603..1192961(+) (pilF) [Cupriavidus gilardii strain NOV2-1]
MLWPSPPFETGRIPVPAGASHTGPLPHCVPSAMIAMTAPSDAPIEYDPAMPSADDAPVVRFVQTLLAEACRRGASDLHFE
PYEGFYRVRFRIDGQLHEVARPPLDIRDRIATRIKVLARLDIAEKRVPQDGRMRFVVPRRDGEATPDGAAGRAVDLRVST
LPTLFGEKIVLRMLAASSVRLDMDSLGLEPTQLAVLLERLRRPHGMVLVTGPTGSGKTVSLYAFLNLLNQHERNISTAED
PAEIQLAGINQVNIHDKIGLNFPTVLRALLRQDPDVIMVGEIRDAETADVAVKAAQTGHLVLSTLHTNDAPGTLTRLAQL
GVAPFHLAASVLVITAQRLARMLCACKRPVPVPLSALREAGFADTLLDGDWQPCQPVGCAACRGSGYRGRCGIHQVMPVS
DAMQNIILSRGSARALAQQARREGVLSLREAGLLKVRAGTTSLAEVLATTNP

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=605833 K6V71_RS14460 WP_420070544.1 1191603..1192961(+) (pilF) [Cupriavidus gilardii strain NOV2-1]
TTGCTTTGGCCGTCCCCGCCATTTGAGACTGGGCGCATTCCCGTTCCCGCCGGCGCATCGCACACTGGGCCGCTCCCGCA
CTGCGTCCCGTCCGCCATGATCGCCATGACCGCCCCGTCCGATGCGCCGATCGAATACGACCCCGCCATGCCATCGGCCG
ACGACGCGCCGGTCGTGCGCTTCGTCCAGACGCTGCTGGCCGAGGCCTGCCGGCGCGGCGCCTCGGACCTGCACTTCGAG
CCGTACGAAGGCTTCTATCGCGTGCGCTTTCGCATCGATGGGCAGCTGCACGAGGTCGCCCGTCCGCCGCTGGACATTCG
CGATCGCATCGCGACGCGGATCAAGGTGCTGGCGCGGCTGGACATCGCCGAGAAGCGTGTACCGCAGGATGGCCGCATGC
GGTTCGTGGTGCCGCGGCGCGACGGCGAGGCGACGCCTGACGGGGCCGCCGGCCGCGCGGTCGATCTGCGCGTATCGACG
CTGCCGACGCTGTTCGGCGAAAAGATCGTGCTGCGCATGCTGGCCGCGTCCAGCGTGCGGCTCGATATGGACAGCCTCGG
CCTCGAGCCGACGCAGCTGGCCGTGCTGCTCGAGCGGCTGCGCCGTCCGCACGGCATGGTGCTGGTCACCGGTCCGACCG
GCAGCGGCAAGACCGTTTCGCTGTACGCCTTCCTGAATCTGCTGAACCAGCACGAGCGCAATATCTCGACGGCCGAGGAC
CCGGCCGAGATCCAGCTGGCCGGCATCAACCAGGTCAATATCCACGACAAGATTGGCCTGAACTTCCCCACCGTGCTGCG
AGCGTTGCTGCGGCAGGACCCCGACGTGATCATGGTCGGCGAGATCCGCGATGCCGAAACCGCCGATGTCGCGGTCAAGG
CGGCGCAGACCGGCCATCTGGTCCTGTCGACGCTGCACACCAACGATGCGCCGGGCACGCTGACACGGCTCGCGCAGCTT
GGCGTCGCGCCCTTTCACCTGGCCGCCAGCGTGCTGGTGATCACCGCGCAGCGGCTGGCCCGCATGCTGTGCGCCTGCAA
ACGTCCGGTCCCGGTGCCGCTGTCCGCGCTGCGCGAGGCCGGTTTCGCGGACACCTTGCTCGACGGCGACTGGCAGCCCT
GCCAGCCGGTCGGCTGCGCCGCGTGCCGCGGCAGCGGTTATCGCGGGCGCTGCGGCATCCATCAGGTGATGCCAGTCTCC
GACGCGATGCAGAACATCATCCTGTCGCGGGGCAGTGCCCGTGCGCTGGCCCAGCAGGCGCGCCGCGAGGGCGTGCTATC
GTTGCGCGAGGCGGGGCTGCTCAAGGTCAGGGCGGGCACGACCTCGCTGGCCGAGGTGCTGGCGACGACCAATCCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(65-342)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57.107

88.717

0.507
  pilB Acinetobacter baumannii D1279779

53.465

89.381

0.478
  pilB Acinetobacter baylyi ADP1

54.198

86.947

0.471
  pilB Vibrio cholerae strain A1552

52.736

88.938

0.469
  pilB Legionella pneumophila strain ERS1305867

49.635

90.929

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

51.01

87.611

0.447
  pilB Vibrio campbellii strain DS40M4

50.758

87.611

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

42.584

92.478

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.68

89.159

0.381
  pilF Thermus thermophilus HB27

41.96

88.053

0.369