Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8O95_RS05360 Genome accession   NZ_CP082887
Coordinates   1118334..1119443 (+) Length   369 a.a.
NCBI ID   WP_223379875.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain FDAARGOS_1459     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1113334..1124443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O95_RS05330 (K8O95_05335) - 1114337..1114756 (-) 420 WP_026457681.1 DUF4426 domain-containing protein -
  K8O95_RS05335 (K8O95_05340) yggU 1114793..1115086 (-) 294 WP_042026655.1 DUF167 family protein YggU -
  K8O95_RS05340 (K8O95_05345) - 1115086..1115637 (-) 552 WP_026457844.1 YggT family protein -
  K8O95_RS05345 (K8O95_05350) proC 1115649..1116473 (-) 825 WP_223379874.1 pyrroline-5-carboxylate reductase -
  K8O95_RS05350 (K8O95_05355) - 1116530..1117231 (-) 702 WP_042026653.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8O95_RS05355 (K8O95_05360) pilT 1117271..1118305 (+) 1035 WP_026457847.1 type IVa pilus ATPase TapT Machinery gene
  K8O95_RS05360 (K8O95_05365) pilU 1118334..1119443 (+) 1110 WP_223379875.1 type IVa pilus ATPase TapU Machinery gene
  K8O95_RS05365 (K8O95_05370) yaaA 1119487..1120260 (+) 774 WP_223379876.1 peroxide stress protein YaaA -
  K8O95_RS05370 (K8O95_05375) - 1120450..1120875 (+) 426 WP_042026650.1 hypothetical protein -
  K8O95_RS05375 (K8O95_05380) - 1121229..1121720 (-) 492 WP_223379877.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  K8O95_RS05380 (K8O95_05385) arfB 1121751..1122167 (-) 417 WP_026456194.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  K8O95_RS05385 (K8O95_05390) glnK 1122369..1122707 (+) 339 WP_026456195.1 P-II family nitrogen regulator -
  K8O95_RS05390 (K8O95_05395) - 1122847..1123317 (-) 471 WP_026456196.1 MarR family winged helix-turn-helix transcriptional regulator -
  K8O95_RS05395 (K8O95_05400) - 1123477..1124004 (+) 528 WP_042070426.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41139.14 Da        Isoelectric Point: 6.2710

>NTDB_id=603365 K8O95_RS05360 WP_223379875.1 1118334..1119443(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1459]
MNLDQMLAELVSRKGSDLFVTVGSPPTLKVNGHLVALGNEPLDKKEALALVRETLSSDHFERYIRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDDLSLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRGIVAQQLVPSIDGRRRFAAFEILLNTPLITDIIRKGEMHRLKEVMTKSSELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGHEQLGSGMLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=603365 K8O95_RS05360 WP_223379875.1 1118334..1119443(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1459]
ATGAATCTGGATCAGATGCTGGCCGAACTGGTCAGCCGCAAGGGCTCGGATCTGTTTGTGACCGTGGGCTCGCCCCCCAC
CCTCAAGGTCAATGGTCACCTGGTGGCACTGGGCAACGAGCCTCTCGACAAGAAGGAGGCGCTGGCCCTGGTCAGGGAGA
CCCTCAGTTCGGACCATTTTGAACGCTACATCCGCACCAAGGAGGCCAACTACGCCATCCATCGCGAGGAGCTGGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGTTGCCTGGCATGGTGCTGCGCCGCATCGAGACCCGTATTCCCACCTTCGA
TGATCTGAGCCTGCCTCCCATATTGCAGGATGTAGCGATGGCCAAGCGGGGACTGGTGCTGTTCGTCGGGGCCACGGGGG
CGGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGCGGATGGCCACATACTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCACCAGCACGGCCGCAGCTTGGTGACCCAGCGCGAGGTGGGGATAGACACCGAATCGTTCGACGT
GGCGCTGAAGAGCTCTCTGCGCCAGGCACCGGATGTGATCCTGATCGGCGAAATCCGTAGCCAGGAGACTATGGAGTTTG
CCCTGCAGTTTGCCGAGACTGGCCACCTCTGTCTGGCAACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTTCTGTTCGATCTCTCGTTCAACCTGCGTGGCATCGTTGCCCAGCA
ACTGGTGCCCAGCATCGACGGACGGCGACGTTTTGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCACCGGCTCAAGGAAGTGATGACCAAATCCTCAGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTGCCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCTCATGCCGACTCGGCCAATGACCTGCGACT
GCTGATCAAGCTTTCGGGTCATGAACAGCTGGGATCCGGCATGCTGGACAATGTGACGCTAGACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

58.689

95.122

0.558
  pilU Acinetobacter baylyi ADP1

56.091

95.664

0.537
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377