Detailed information NTG0600766

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K6I41_RS14840
• Genome accession: NZ_CP082274
• Coordinates: 3144494..3145612 (+)
• Length: 372 a.a.
• NCBI ID: WP_013198760.1
• Uniprot ID: A0AAN0UEB6
• Organism: Acinetobacter sp. AS23
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3139494..3150612

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6I41_RS14825 (K6I41_14710)	-	3141668..3142510 (-)	843	WP_151767746.1	undecaprenyl-diphosphate phosphatase	-
K6I41_RS14830 (K6I41_14715)	-	3142618..3143310 (-)	693	WP_250374702.1	YggS family pyridoxal phosphate-dependent enzyme	-
K6I41_RS14835 (K6I41_14720)	pilT	3143437..3144474 (+)	1038	WP_003653239.1	type IV pilus twitching motility protein PilT	Machinery gene
K6I41_RS14840 (K6I41_14725)	pilU	3144494..3145612 (+)	1119	WP_013198760.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K6I41_RS14845 (K6I41_14730)	fur	3145682..3146119 (-)	438	WP_004794120.1	ferric iron uptake transcriptional regulator	-
K6I41_RS14850 (K6I41_14735)	bamE	3146231..3146629 (+)	399	WP_003653245.1	outer membrane protein assembly factor BamE	-
K6I41_RS14855 (K6I41_14740)	-	3146694..3147008 (-)	315	WP_250374703.1	RnfH family protein	-
K6I41_RS14860 (K6I41_14745)	-	3147008..3148075 (-)	1068	WP_250374704.1	hypothetical protein	-
K6I41_RS14865 (K6I41_14750)	-	3148227..3148682 (-)	456	WP_013198763.1	bacteriohemerythrin	-
K6I41_RS14870 (K6I41_14755)	-	3148932..3149780 (-)	849	WP_013198764.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41887.10 Da
• Isoelectric Point: 6.6401

>NTDB_id=600766 K6I41_RS14840 WP_013198760.1 3144494..3145612(+) (pilU) [Acinetobacter sp. AS23]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=600766 K6I41_RS14840 WP_013198760.1 3144494..3145612(+) (pilU) [Acinetobacter sp. AS23]
ATGGATTTTAATGACTTACTCAACCTTATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAGAATAGTCTTTCAGGCGAAGTAATTGGTCAACTTTTACATTCCA
TCATGAGCGAAAAACAGCGCAAAGAATTTGCCGACACTCGTGAATGTAACTTTGCCATTATGAACCGCGATAAAACTGCC
CGCTTTCGTGTGAGCGCTTTTCAACAGCGTGACATGCCAGGAATGGTATTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCGCCCGTTCTTAAAGATTTATCAATGACCAAACGCGGCATCATTATTTTTGTTGGTGCGACAG
GTACGGGTAAATCGACGTCTTTAGCTTCAATGATTAGCTATCGTAACCACAACACTAAAGGTCATATCATTACCATTGAA
GATCCAATCGAGTTTATTCACGAACATGCAGGCTGCATCATTACCCAACGTGAAGTCGGAATCGATACGGATTCATTTGA
AATTGCCTTAAAAAATACTTTGCGACAAGCACCAGATGTCATCTTGATTGGTGAGATTCGATCTCGTGAAGTTATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTGGCCACAATGCACGCCAACAATGCTAACCAAGCACTCGACCGT
ATCATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACACCTGATGGCAATTCTCGCCGCGCCGCAATTGAAATTTTAATTAACTCACCATTAATTTCAGATT
ACATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGTTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCATTGTTTGATCTCTATAAAGCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCTGACTCACCAAATGATTTACG
TTTGACGATTAAGCTTGCAGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	74.868	100	0.761
pilU	Pseudomonas stutzeri DSM 10701	67.925	99.731	0.677
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	97.849	0.398
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36