Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K7B08_RS07415 Genome accession   NZ_CP082272
Coordinates   1485164..1486549 (-) Length   461 a.a.
NCBI ID   WP_223131888.1    Uniprot ID   A0AAW8V5M2
Organism   Pasteurella multocida strain 17BRD-035     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1480164..1491549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7B08_RS07385 - 1480291..1481664 (-) 1374 WP_195188374.1 sodium:alanine symporter family protein -
  K7B08_RS07390 - 1482113..1482382 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  K7B08_RS07395 yacG 1482382..1482585 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  K7B08_RS07400 coaE 1482596..1483216 (-) 621 WP_005753357.1 dephospho-CoA kinase -
  K7B08_RS07405 - 1483231..1483953 (-) 723 WP_223131886.1 A24 family peptidase -
  K7B08_RS07410 - 1483953..1485164 (-) 1212 WP_223131887.1 type II secretion system F family protein -
  K7B08_RS07415 pilB 1485164..1486549 (-) 1386 WP_223131888.1 GspE/PulE family protein Machinery gene
  K7B08_RS07420 pilA 1486549..1486983 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  K7B08_RS07425 ampD 1487143..1487709 (+) 567 WP_014668228.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K7B08_RS07430 rppH 1488295..1488762 (+) 468 Protein_1438 RNA pyrophosphohydrolase -
  K7B08_RS07435 - 1488894..1489688 (+) 795 WP_005719128.1 sulfite exporter TauE/SafE family protein -
  K7B08_RS07440 lgt 1489698..1490501 (+) 804 WP_014668230.1 prolipoprotein diacylglyceryl transferase -
  K7B08_RS07445 - 1490507..1491358 (+) 852 WP_223131889.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52092.74 Da        Isoelectric Point: 6.3686

>NTDB_id=600717 K7B08_RS07415 WP_223131888.1 1485164..1486549(-) (pilB) [Pasteurella multocida strain 17BRD-035]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=600717 K7B08_RS07415 WP_223131888.1 1485164..1486549(-) (pilB) [Pasteurella multocida strain 17BRD-035]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCGCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.811

98.482

0.579
  pilB Acinetobacter baumannii D1279779

40.162

100

0.429
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.412
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395