Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K7B08_RS07420 Genome accession   NZ_CP082272
Coordinates   1486549..1486983 (-) Length   144 a.a.
NCBI ID   WP_061406215.1    Uniprot ID   Q9LA12
Organism   Pasteurella multocida strain 17BRD-035     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1481549..1491983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7B08_RS07390 - 1482113..1482382 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  K7B08_RS07395 yacG 1482382..1482585 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  K7B08_RS07400 coaE 1482596..1483216 (-) 621 WP_005753357.1 dephospho-CoA kinase -
  K7B08_RS07405 - 1483231..1483953 (-) 723 WP_223131886.1 A24 family peptidase -
  K7B08_RS07410 - 1483953..1485164 (-) 1212 WP_223131887.1 type II secretion system F family protein -
  K7B08_RS07415 pilB 1485164..1486549 (-) 1386 WP_223131888.1 GspE/PulE family protein Machinery gene
  K7B08_RS07420 pilA 1486549..1486983 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  K7B08_RS07425 ampD 1487143..1487709 (+) 567 WP_014668228.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K7B08_RS07430 rppH 1488295..1488762 (+) 468 Protein_1438 RNA pyrophosphohydrolase -
  K7B08_RS07435 - 1488894..1489688 (+) 795 WP_005719128.1 sulfite exporter TauE/SafE family protein -
  K7B08_RS07440 lgt 1489698..1490501 (+) 804 WP_014668230.1 prolipoprotein diacylglyceryl transferase -
  K7B08_RS07445 - 1490507..1491358 (+) 852 WP_223131889.1 thymidylate synthase -
  K7B08_RS07450 tadA 1491385..1491873 (+) 489 WP_223131890.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15213.68 Da        Isoelectric Point: 9.1215

>NTDB_id=600718 K7B08_RS07420 WP_061406215.1 1486549..1486983(-) (pilA) [Pasteurella multocida strain 17BRD-035]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNTPSECSAGQHGIR
ANISSGKKYLSSINVKSGVITVIGQKSLEGVNYTLSATGNAVDGVSWSVKCNGGSEVFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=600718 K7B08_RS07420 WP_061406215.1 1486549..1486983(-) (pilA) [Pasteurella multocida strain 17BRD-035]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCTACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATACGCCCAGTGAATGTAGTGCAGGACAGCATGGTATTAGA
GCAAATATAAGTTCTGGTAAAAAATATCTCAGCTCAATCAATGTCAAGTCTGGCGTGATTACTGTCATTGGTCAAAAAAG
CTTAGAGGGAGTCAATTACACTTTAAGCGCGACAGGAAATGCCGTTGATGGTGTTTCATGGAGTGTAAAATGTAATGGTG
GTAGTGAAGTTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9LA12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

59.028

100

0.59
  pilA Haemophilus influenzae Rd KW20

59.028

100

0.59
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  comP Acinetobacter baylyi ADP1

37.681

95.833

0.361