Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BAJP3042_RS12540 Genome accession   NZ_CP082243
Coordinates   2605239..2605853 (-) Length   204 a.a.
NCBI ID   WP_222835183.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP3042     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2600239..2610853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAJP3042_RS12510 (BAJP3042_12510) rpsT 2600542..2600808 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  BAJP3042_RS12515 (BAJP3042_12515) holA 2600825..2601866 (-) 1042 Protein_2436 DNA polymerase III subunit delta -
  BAJP3042_RS12520 (BAJP3042_12520) - 2601906..2602057 (-) 152 Protein_2437 hypothetical protein -
  BAJP3042_RS12525 (BAJP3042_12525) - 2602098..2602232 (+) 135 WP_003152870.1 YqzM family protein -
  BAJP3042_RS12530 (BAJP3042_12530) comEC 2602251..2604602 (-) 2352 WP_222835182.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BAJP3042_RS12535 (BAJP3042_12535) - 2604603..2605172 (-) 570 WP_003152868.1 ComE operon protein 2 -
  BAJP3042_RS12540 (BAJP3042_12540) comEA 2605239..2605853 (-) 615 WP_222835183.1 helix-hairpin-helix domain-containing protein Machinery gene
  BAJP3042_RS12545 (BAJP3042_12545) comER 2605912..2606733 (+) 822 WP_012118027.1 late competence protein ComER -
  BAJP3042_RS12550 (BAJP3042_12550) - 2606802..2607539 (-) 738 WP_222835184.1 class I SAM-dependent methyltransferase -
  BAJP3042_RS12555 (BAJP3042_12555) rsfS 2607536..2607892 (-) 357 WP_007408260.1 ribosome silencing factor -
  BAJP3042_RS12560 (BAJP3042_12560) yqeK 2607910..2608470 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BAJP3042_RS12565 (BAJP3042_12565) - 2608460..2609029 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  BAJP3042_RS12570 (BAJP3042_12570) yhbY 2609040..2609330 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  BAJP3042_RS12575 (BAJP3042_12575) aroE 2609324..2610166 (-) 843 WP_222835185.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22122.28 Da        Isoelectric Point: 5.5107

>NTDB_id=600461 BAJP3042_RS12540 WP_222835183.1 2605239..2605853(-) (comEA) [Bacillus amyloliquefaciens strain JP3042]
MTKAMMTERLLRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=600461 BAJP3042_RS12540 WP_222835183.1 2605239..2605853(-) (comEA) [Bacillus amyloliquefaciens strain JP3042]
ATGACGAAAGCGATGATGACGGAGCGGCTGCTGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.377

100

0.368
  comEA/celA/cilE Streptococcus mitis NCTC 12261

35.377

100

0.368
  comEA/celA/cilE Streptococcus pneumoniae Rx1

35.377

100

0.368
  comEA/celA/cilE Streptococcus pneumoniae D39

35.377

100

0.368
  comEA/celA/cilE Streptococcus pneumoniae R6

35.377

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

34.906

100

0.363
  comEA Latilactobacillus sakei subsp. sakei 23K

33.484

100

0.363