Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5Y35_RS22925 Genome accession   NZ_CP082236
Coordinates   5138086..5139192 (-) Length   368 a.a.
NCBI ID   WP_128614074.1    Uniprot ID   -
Organism   Pseudomonas sp. SC3(2021)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5133086..5144192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5Y35_RS22910 - 5133843..5135234 (+) 1392 WP_222833035.1 GntP family permease -
  K5Y35_RS22915 hbdH 5135242..5136015 (+) 774 WP_222833036.1 3-hydroxybutyrate dehydrogenase -
  K5Y35_RS22920 - 5136112..5138067 (+) 1956 WP_222833037.1 acetoacetate--CoA ligase -
  K5Y35_RS22925 pilU 5138086..5139192 (-) 1107 WP_128614074.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5Y35_RS22930 - 5139324..5141861 (+) 2538 WP_222833038.1 PAS domain-containing sensor histidine kinase -
  K5Y35_RS22935 - 5141913..5142188 (+) 276 WP_007912322.1 peptidylprolyl isomerase -
  K5Y35_RS22940 - 5142303..5143235 (-) 933 WP_222833039.1 sugar kinase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40908.92 Da        Isoelectric Point: 6.8358

>NTDB_id=600364 K5Y35_RS22925 WP_128614074.1 5138086..5139192(-) (pilU) [Pseudomonas sp. SC3(2021)]
MEIDALLTRLASQHGSDLFLSTGAPPSARFQGVLTPLAEQPFKPGEVAVIANSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNLFKQRNDVSIVIRNVKLDIPRFEDLKLPRVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PIEYIHRHRLSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
INLFAEERRPQLLHALGNNLKAFVSQRLVRTLEGQRRAAVEVMLGTPTIGDLIRRNELGELKGIMEKSTELGMQTFDGAL
HGLVVEGAISEEEALKHADSVNNLRLRLKLQGDVTPAPPTTGEWGLMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=600364 K5Y35_RS22925 WP_128614074.1 5138086..5139192(-) (pilU) [Pseudomonas sp. SC3(2021)]
ATGGAAATCGATGCACTGTTAACACGCTTGGCCAGCCAGCATGGCTCGGACCTGTTCCTGTCGACCGGAGCGCCACCCAG
CGCGCGCTTTCAAGGCGTATTGACGCCGTTGGCCGAGCAGCCGTTCAAGCCGGGTGAAGTCGCGGTCATCGCCAATTCCC
TGATGGACGCCGAACAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCCATCTCGCGCACCGGCATCGGCCGC
TTTCGGGTCAATCTGTTCAAGCAACGCAATGACGTGTCGATCGTGATCCGCAACGTCAAACTGGACATTCCGCGCTTTGA
AGACCTCAAGTTGCCCCGCGTGCTGCTGGAAACCGTGATGCTCAAACAAGGCCTGATTCTGTTTGTCGGCGCCACCGATT
CGGGCAAATCGACTTCGCTGGCGGCGCTGATCGATCATCGCAATCGCCATAGCAGTGGCCACATCATCACGATTGAAGAC
CCAATCGAGTACATCCACCGGCATCGGCTGTCGATCATCAACCAGCGTGAAGTCGGTGTCGACACCCGCAGCTTTCACGC
GGCTTTGAAAAACACTCTGCGCCAGGCACCCGATGTGGTGCTGATCGGCGAAATCCGCGACCGCGAGACCATGGAGCACG
CGCTGGCGTTCGCCGAAACCGGTCATCTGGTTTTATCGACGCTGCACGCCAACAACGCCAATCAGGCGCTCGACCGGATC
ATCAACCTGTTTGCCGAGGAGCGTCGGCCACAGTTGCTGCATGCACTGGGCAACAACCTTAAAGCCTTTGTTTCCCAGCG
CCTGGTGCGCACGCTCGAGGGGCAGCGGCGGGCAGCCGTGGAAGTGATGCTGGGCACGCCGACCATCGGGGACCTGATCC
GGCGTAATGAATTGGGTGAGCTCAAAGGCATCATGGAAAAGTCCACCGAACTGGGCATGCAGACGTTCGACGGGGCGTTG
CACGGCCTGGTGGTGGAGGGTGCGATCAGTGAGGAGGAGGCACTCAAACATGCCGATTCGGTGAACAATCTGCGCCTGCG
CTTGAAGTTGCAGGGCGATGTCACACCGGCCCCACCCACGACGGGGGAGTGGGGCCTGATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(70-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.131

99.728

0.56
  pilU Acinetobacter baylyi ADP1

54.261

95.652

0.519
  pilU Vibrio cholerae strain A1552

51.549

96.467

0.497
  pilT Pseudomonas aeruginosa PAK

39.244

93.478

0.367
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.361

90.217

0.364
  pilT Pseudomonas stutzeri DSM 10701

38.953

93.478

0.364