Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   L897_RS00655 Genome accession   NC_021807
Coordinates   104135..105169 (+) Length   344 a.a.
NCBI ID   WP_236608388.1    Uniprot ID   -
Organism   Streptococcus pyogenes HSC5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99135..110169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L897_RS00645 (L897_00670) - 102803..103168 (+) 366 WP_002986560.1 DUF1033 family protein -
  L897_RS00650 (L897_00675) comYA 103261..104199 (+) 939 WP_002993471.1 competence type IV pilus ATPase ComGA Machinery gene
  L897_RS00655 (L897_00680) comYB 104135..105169 (+) 1035 WP_236608388.1 competence type IV pilus assembly protein ComGB Machinery gene
  L897_RS00660 (L897_00685) comYC 105171..105497 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  L897_RS00665 (L897_00690) comGD 105472..105900 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  L897_RS00670 (L897_00695) comGE 105857..106141 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  L897_RS00675 (L897_00700) comYF 106134..106568 (+) 435 WP_020833199.1 competence type IV pilus minor pilin ComGF Machinery gene
  L897_RS00680 (L897_00705) comGG 106552..106878 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  L897_RS00685 (L897_00710) comYH 106976..107929 (+) 954 WP_042361463.1 class I SAM-dependent methyltransferase Machinery gene
  L897_RS00690 (L897_00715) - 107988..109184 (+) 1197 WP_020833201.1 acetate kinase -
  L897_RS09080 (L897_00720) - 109371..109679 (+) 309 Protein_92 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39691.21 Da        Isoelectric Point: 9.7691

>NTDB_id=59904 L897_RS00655 WP_236608388.1 104135..105169(+) (comYB) [Streptococcus pyogenes HSC5]
MISFLRQDISIPSKLSLKKLSSKRQYKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMAILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=59904 L897_RS00655 WP_236608388.1 104135..105169(+) (comYB) [Streptococcus pyogenes HSC5]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACGTCAGTATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGGCTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment